专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06271381B1 Cocaine derivative, protein conjugate thereof, monoclonal antibody producing cell line, method for preparing the cell line and monoclonal antibody 失效
标题翻译：可卡因衍生物，其蛋白质缀合物，产生单克隆抗体的细胞系，制备细胞系的方法和单克隆抗体
公开(公告)号：US06271381B1
公开(公告)日：2001-08-07
申请号：US09706774
申请日：2000-11-07
申请人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C07D40100
CPC分类号： G01N33/946 , A61K47/60 , A61K47/643 , C07D451/12 , C07K16/16
摘要： A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives.
摘要翻译：通过使用具有甲氧基羰基和苯甲酰基的可卡因衍生物容易地制备可卡因 - 蛋白质结合物的方法。该缀合物可用于检测可卡因或可卡因衍生物。

2. 发明授权

US5378634A Labelling color for detecting methamphetamine 失效
标题翻译：用于检测甲基苯丙胺的标签颜色
公开(公告)号：US5378634A
公开(公告)日：1995-01-03
申请号：US102426
申请日：1993-08-05
申请人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
发明人： Nobuyuki Sigetoh , Jinsei Miyazaki , Hiroshi Nakayama , Keiko Yugawa , Tadayasu Mitsumata
IPC分类号： C09B23/08 , G01N33/58 , G01N33/00
CPC分类号： C09B23/083 , G01N33/582 , Y10S435/968 , Y10S436/816 , Y10S436/901 , Y10T436/14
摘要： A labelling color which includes a pentamethine of the following formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.
摘要翻译：包括下式（1）的五甲氧基的标记颜色：其中R 1，R 2，R 3和R 4各自为C 1-6烷基，X为阴离子。

3. 发明授权

US5571727A Labelling colors for detecting cocaine or methamphetamine, method of preparing the same and detector for cocaine or methamphetamine 失效
标题翻译：用于检测可卡因或甲基苯丙胺的标签颜色，其制备方法和可卡因或甲基苯丙胺检测器
公开(公告)号：US5571727A
公开(公告)日：1996-11-05
申请号：US319976
申请日：1994-10-07
申请人： Nobuyuki Shigetoh , Hiroshi Nakayama , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Nobuyuki Shigetoh , Hiroshi Nakayama , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C07D209/14 , C07D471/08 , G01N33/532 , G01N33/542 , G01N33/94 , C07D401/06 , G01N33/533
CPC分类号： C07D471/08 , C07D209/14 , G01N33/532 , G01N33/542 , G01N33/946 , Y10S436/816
摘要： A labelling color for detecting cocaine of Formula (I) ##STR1## wherein X represents a halogen, and a method of detecting methamphetamine, including the steps of adding a labelling color of Formula (II) ##STR2## wherein X represents an anion to a first solution of methamphetamine antibody to form a labelling color-methamphetamine antibody complex, measuring the fluorescence of the first solution, adding a sample containing an unknown amount of methamphetamine to the first solution to form a second solution, measuring the fluorescence of the second solution, and correlating any change in the fluorescence between the first and second solutions to the concentration of methamphetamine in the sample.
摘要翻译：用于检测式（I）的可卡因的标记颜色其中X表示卤素的式（I）和检测甲基苯丙胺的方法，包括以下步骤：添加式（II）的标记颜色），其中X表示甲基苯丙胺抗体的第一溶液的阴离子，以形成标记颜色 - 甲基苯丙胺抗体复合物，测量第一溶液的荧光，向第一溶液中加入含有未知量的甲基苯丙胺的样品以形成第二溶液，测量第二溶液的荧光，并将第一和第二溶液之间的荧光的任何变化与样品中甲基苯丙胺的浓度相关联。

4. 发明授权

US06174723B1 Cocaine derivative, protein conjugate thereof, monoclonal antibody producing cell line, method for preparing the cell line and monoclonal antibody 失效
标题翻译：可卡因衍生物，其蛋白质缀合物，产生单克隆抗体的细胞系，制备细胞系的方法和单克隆抗体
公开(公告)号：US06174723B1
公开(公告)日：2001-01-16
申请号：US08982963
申请日：1997-12-02
申请人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
发明人： Keiko Yugawa , Nobuyuki Sigetoh , Jinsei Miyazaki , Tadayasu Mitsumata
IPC分类号： C12N506
CPC分类号： G01N33/946 , A61K47/60 , A61K47/643 , C07D451/12 , C07K16/16
摘要： A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives. A monoclonal antibody, a monoclonal antibody producing cell line, and a method for producing the monoclonal antibody producing cell line by using the above cocaine-protein conjugate as an immunogen is also described. The method of the invention comprises preparing a cocaine-protein conjugate with a pyridyl dithiococaine derivative of Formula (I): where R is either H or R′; and where R′ is: wherein n=1 to 4 and Ph is a benzene ring, and wherein said monoclonal antibody detects 10−9 M cocaine; and which derivative binds to a protein by a disulfide bond; immunizing A/J mouse with the conjugate; fusing its spleen cell and a myeloma cell line; cloning the fusion to produce a monoclonal antibody producing cell line; culturing the cell line, and purifying a supernatant of the culture to form a monoclonal antibody to specifically bind to cocaine or cocaine derivatives.
摘要翻译：通过使用具有甲氧基羰基和苯甲酰基的可卡因衍生物容易地制备可卡因 - 蛋白质结合物的方法。该缀合物可用于检测可卡因或可卡因衍生物。还描述了单克隆抗体，产生单克隆抗体的细胞系，以及通过使用上述可卡因蛋白质缀合物作为免疫原来制备产生单克隆抗体的细胞系的方法。本发明的方法包括用式（I）的吡啶基二硫代可卡因衍生物制备可卡因蛋白质结合物：其中R是H或R'; 其中R'为：其中n = 1-4，Ph为苯环，其中所述单克隆抗体检测10-9M可卡因; 哪个衍生物通过二硫键与蛋白质结合; 用缀合物免疫A / J小鼠; 融合其脾细胞和骨髓瘤细胞系; 克隆融合物以产生产生单克隆抗体的细胞系; 培养细胞系，并纯化培养物的上清液以形成特异性结合可卡因或可卡因衍生物的单克隆抗体。

5. 发明授权

US5243074A Immunoreagents 失效
标题翻译：免责声明
公开(公告)号：US5243074A
公开(公告)日：1993-09-07
申请号：US616655
申请日：1990-11-21
申请人： Tadayasu Mitsumata , Jinsei Miyazaki
发明人： Tadayasu Mitsumata , Jinsei Miyazaki
IPC分类号： G01N33/533 , G01N33/58
CPC分类号： G01N33/533 , G01N33/582 , Y10S436/815 , Y10S436/816 , Y10S436/901
摘要： An immunofluorescent assay method, characterized in using an immunodetective reagent, which is an antigen-like substance comprising a fluorescent substance and an antigen chemically bonded thereto with or without intervention of an additional chemical bond, said fluorescent substance being changed in the wavelength or intensity of the fluorescence when an antibody comes close thereto, and said antigen being specific to said antibody.

6. 发明授权

US5233025A Amphetamine protein complex as immunogen for obtaining antibodies specific to methamphetamine 失效
标题翻译：安非他明蛋白复合物作为获得甲基苯丙胺特异性抗体的免疫原
公开(公告)号：US5233025A
公开(公告)日：1993-08-03
申请号：US651809
申请日：1991-02-07
申请人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： A61K47/48 , C07K16/44 , G01N33/531
CPC分类号： G01N33/531 , A61K47/4833 , C07K16/44
摘要： A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a higher affinity to methamphetamine that to the hapten N-(4-aminobutyl)amphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepeared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.
摘要翻译：已经合成了N-（4-氨基丁基）苯丙胺 - 蛋白质复合物作为免疫原，以制备对半抗原N-（4-氨基丁基）苯丙胺具有较高的对甲基苯丙胺的亲和力的抗体。通过使用该复合物制备的抗体与通过使用N-（4-氨基丁基）甲基苯丙胺 - 蛋白复合物作为免疫原而展示的抗体的甲基苯丙胺的亲和力为1,000倍。

7. 发明授权

US5229302A Fluorescence immunoassay method utilizing pseudo-antigens combined with fluorescent quenchers 失效
标题翻译：荧光免疫测定法利用假抗原与荧光猝灭剂结合
公开(公告)号：US5229302A
公开(公告)日：1993-07-20
申请号：US830442
申请日：1992-02-03
申请人： Jinsei Miyazaki , Noboru Motoyama , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Noboru Motoyama , Tadayasu Mitsumata
IPC分类号： G01N33/532 , G01N33/542 , G01N33/94
CPC分类号： G01N33/542 , G01N33/532 , G01N33/946 , Y10S436/816
摘要： A fluorescence immunoassay method is described in which the presence or absence of antigen is measured within one minute by measuring a variation in fluorescence emitted from an antibody. The method comprises reaction between a pseudo-antigen obtained by chemical combination of an antigen and a fluorescent quencher and an antibody by which fluorescence to be emitted from the antibody is quenched by the action of the quencher combined. When an analyte is added to a solution of the combination, the pseudo-antigen is replaced by an antigen if the analyte contains the antigen, so that the fluorescence increases in intensity. This intensity is measured and compared with the initially measured intensity to detect the presence of the antigen. The method does not make use of the action of antigen on antibody with respect to the fluorescence of the antibody and is applicable to all antigens.
摘要翻译：描述了荧光免疫测定方法，其中通过测量从抗体发射的荧光的变化，在一分钟内测量抗原的存在或不存在。该方法包括通过抗原和荧光猝灭剂的化学组合获得的假抗原与通过猝灭剂组合的作用淬灭从抗体发射的荧光的抗体之间的反应。当将分析物加入到组合的溶液中时，如果分析物含有抗原，假抗原被抗原替代，使得荧光强度增加。测量该强度并与初始测量的强度进行比较以检测抗原的存在。该方法没有利用抗体对抗体对抗体的荧光的作用，适用于所有抗原。

8. 发明授权

US5026827A Amphetamine-protein complex as immunogen for obtaining antibodies specific to methamphetamine 失效
标题翻译：安非他明 - 蛋白复合物作为获得甲基苯丙胺特异性抗体的免疫原
公开(公告)号：US5026827A
公开(公告)日：1991-06-25
申请号：US401247
申请日：1989-08-31
申请人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： A61K47/48 , C07K16/44 , G01N33/531
CPC分类号： G01N33/531 , A61K47/4833 , C07K16/44
摘要： A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a high affinity to methamphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.

9. 发明授权

US5810725A Planar electrode 失效
标题翻译：平面电极
公开(公告)号：US5810725A
公开(公告)日：1998-09-22
申请号：US481149
申请日：1995-06-07
申请人： Hirokazu Sugihara , Makoto Taketani , Tadayasu Mitsumata
发明人： Hirokazu Sugihara , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： A61N1/04 , A61N1/05 , G01N33/483 , G01N33/487 , A61B5/04
CPC分类号： G01N33/48728 , A61N1/0456 , A61N1/0476 , A61N1/0496 , A61N1/0551 , A61N1/0464 , A61N1/0488 , A61N1/0553
摘要： A planar electrode which enables multi-point simultaneous stimulation and recording of nerve cells over a long time is provided, which also has excellent response property. An ITO film is evaporated on the whole surface of an insulating substrate of the hard glass, and the ITO film is etched so that the central portion of each electrode is located on each intersection of 8.times.8 lattices, the center-to-center distances of nearest electrodes of each electrode are equal, and a lead wire is stretched radially. Then, for an insulating layer, negative photo-sensitive polyimide is spin-coated, and an insulating layer pattern is exposure-formed so that a 50 .mu.ms square hole is produced at the center of each electrode. Furthermore, to the exposed portion of each electrode (that is, the inside of the 50 .mu.ms square), Ni is evaporated in a film thickness of 500 nm, which is followed by evaporating gold (50 nm) and platinum black (about 1 .mu.m ). The contact with the external circuit of the section near the end opposite to the electrode of the lead wire was coated with gold and nickel.
摘要翻译：提供了能够长时间多点同时刺激和记录神经细胞的平面电极，其也具有优异的响应特性。在硬质玻璃的绝缘基板的整个表面上蒸发ITO膜，蚀刻ITO膜，使得每个电极的中心部分位于8×8晶格的每个交点上，中心距离最近每个电极的电极相等，引线径向拉伸。然后，对于绝缘层，旋转涂覆负光敏聚酰亚胺，并且将曝光形成绝缘层图案，使得在每个电极的中心处产生50微米的方孔。此外，对于每个电极的曝光部分（即，50μs均方的内部），以500nm的膜厚度蒸发Ni，然后蒸发金（50nm）和铂黑（约1 亩）。与导线的电极相对的端部附近的部分的外部电路的接触涂覆有金和镍。

10. 再颁专利

USRE40209E1 Cell potential measurement apparatus having a plurality of microelectrodes 有权
标题翻译：具有多个微电极的电池电位测量装置
公开(公告)号：USRE40209E1
公开(公告)日：2008-04-01
申请号：US09688077
申请日：2000-10-13
申请人： Hirokazu Sugihara , Akihito Kamei , Yasushi Kobayashi , Makoto Taketani , Tadayasu Mitsumata
发明人： Hirokazu Sugihara , Akihito Kamei , Yasushi Kobayashi , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： C12M1/34
CPC分类号： C12M41/46 , G01N33/48728
摘要： A cell potential measurement apparatus, which uses a planar electrode enabling a multi-point simultaneous measurement of potential change arising from cell activities, is provided which can conduct measurements accurately and efficiently as well as can improve convenience of arranging measurement results. According to the configuration of the cell potential measurement apparatus of this invention, it includes an integrated cell holding instrument, which includes a planar electrode provided with a plurality of microelectrodes arranged in a matrix form on the surface of a substrate, a cell holding part for placing cells thereon, drawer patterns from the microelectrodes, and electric contact points for outside connections; an optical observation means for optical observations of cells; a stimulation signal supply means to be connected to the cell holding instrument for providing electric stimulation to the cells; and a signal processing means to be connected to the cell holding instrument for processing an output signal arising from electric physiological activities of the cells. It is preferable that a cell culturing means is also provided for maintaining a culture atmosphere of the cells placed on the integrated cell holding instrument.
摘要翻译：提供一种电池电位测量装置，其使用能够进行多点同时测量由电池活动引起的电位变化的平面电极，其可以精确且有效地进行测量，并且可以提高排列测量结果的便利性。根据本发明的电池电位测量装置的结构，它包括一个集成的电池保持仪器，它包括一个平面电极，该平面电极设置有以矩阵形式布置在基片表面上的多个微电极，在其上放置电池，来自微电极的抽屉图案和用于外部连接的电接触点; 用于细胞光学观察的光学观察装置; 刺激信号提供装置，连接到用于向细胞提供电刺激的细胞保持仪器; 以及信号处理装置，其连接到用于处理由电池的电生理活动引起的输出信号的电池保持装置。还优选还设置有用于维持放置在集成电池保持器具上的电池的培养气氛的细胞培养装置。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式