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    • 9. 发明授权
    • DNA analysis apparatus
    • DNA分析仪器
    • US08975066B2
    • 2015-03-10
    • US12419399
    • 2009-04-07
    • Hideki KambaraAkihiko Kishimoto
    • Hideki KambaraAkihiko Kishimoto
    • C12M1/34C12M3/00C12Q1/68B01L7/00
    • C12Q1/6869B01J2219/00659B01J2219/00722B01L7/52C12Q2565/301C12Q2563/103C12Q2535/101
    • Accurate and sensitive sequencing in pyrosequencing is achieved by allowing complementary strand synthesis reaction to proceed homogeneously and completely in a short time while performing luminescence reaction for a sufficiently long time. DNA as a sequencing target is immobilized on the surface of a solid support. Nucleic acid substrates are injected from a dispenser to the support site where complementary strand synthesis is in turn performed rapidly and completely in a short time under a small reaction volume. Next, the support together with the product thereon is moved into a luminescence reaction solution where luminescence reaction is in turn performed. Thus, a DNA complementary strand synthesis reaction site and a luminescence reaction site are completely separated. The support surface is also washed by dipping the support in the luminescence reaction solution that contains a luminescence reagent and an enzyme that degrades redundant nucleic acid substrates.
    • 焦磷酸测序中的精确和灵敏的测序通过使互补链合成反应在短时间内均匀且完全地进行,同时进行发光反应足够长的时间来实现。 作为测序靶的DNA被固定在固体支持物的表面上。 将核酸底物从分配器注射到支持部位,其中互补链合成在短时间内在小反应体积下快速且完全地进行。 接下来,将载体与其上的产物一起移动到进行发光反应的发光反应溶液中。 因此,DNA互补链合成反应位点和发光反应位点完全分离。 通过将载体浸渍在含有发光试剂的发光反应溶液和降解冗余核酸底物的酶中也可以洗涤载体表面。