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    • 4. 发明授权
    • Modified glucose dehydrogenase gene
    • 改良葡萄糖脱氢酶基因
    • US08445221B2
    • 2013-05-21
    • US12810213
    • 2008-12-26
    • Michinari HondaRyo TakenakaFuminao Kobayashi
    • Michinari HondaRyo TakenakaFuminao Kobayashi
    • C12Q1/54C12N9/00C12N9/02C12N1/20C07H21/04
    • C12Q1/32C12N9/0006C12Q1/006
    • [Object] To provide an FAD-conjugated glucose dehydrogenase having a higher specificity for glucose.[Means for Resolution] A modified glucose dehydrogenase (GLD) which includes a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 37, 69, 72, 73, 76, 78, 102, 217, 228, 240, 356, 407, 424, 437, 527, and 530 in an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1, and has a decreased ratio of activity for xylose/activity for glucose as compared with the wild-type GLD; a polynucleotide encoding the modified GLD; a recombinant vector containing the polynucleotide; a transformed cell produced by using the recombinant vector; etc.
    • 提供对葡萄糖具有较高特异性的FAD-缀合的葡萄糖脱氢酶。 [解决方法]一种修饰的葡萄糖脱氢酶(GLD),其包括至少一个氨基酸残基的取代,所述氨基酸残基选自第37,69,72,73,76,78,102,217, 在由SEQ ID NO:1表示的野生型FAD-缀合的葡萄糖脱氢酶(GLD)的氨基酸序列中的228,240,356,407,424,473,527和530,并且具有降低的 与野生型GLD相比,葡萄糖的木糖/活性; 编码经修饰的GLD的多核苷酸; 含有多核苷酸的重组载体; 通过使用重组载体产生的转化细胞; 等等