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1. 发明授权

US07556962B2 Saccharide composition purification instrument 有权
标题翻译：糖组成净化仪
公开(公告)号：US07556962B2
公开(公告)日：2009-07-07
申请号：US11061536
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12M1/14 , C12M3/04
CPC分类号： C12P19/00 , B01J20/3242 , B01J2220/603 , C12N11/00 , C12P21/005 , C12P21/06
摘要： The present invention provides a sugar chain purification instrument which can continuously and efficiently purify the sugar chain. According to the present invention, the instrument includes an immobilized protease column in which a carrier which can be packed in the column and on which protease is immobilized is packed and an immobilized glycopeptidase column in which a carrier which can be packed in the column and on which glycopeptidase is immobilized is packed, these columns being connected in series, and the reaction is carried out while continuously passing a glycoprotein to be decomposed together with a suitable buffer solution through the above columns. The step of liberation of sugar chain from the glycoprotein is highly simplified and labor-saving, and liberation of the sugar chain can be moderately and simply performed.
摘要翻译：本发明提供一种可连续高效地纯化糖链的糖链纯化装置。根据本发明，仪器包括固定化的蛋白酶柱，其中可以填充在柱中并且固定有蛋白酶的载体和固定的糖肽酶柱，其中可以包装在柱中的载体和将这些糖肽酶固定化，将这些柱串联连接，在连续通过糖蛋白的同时进行反应，并通过上述柱与合适的缓冲溶液一起分解。从糖蛋白释放糖链的步骤被高度简化和省力，可以适度简单地进行糖链的释放。

2. 发明申请

US20060121559A1 Saccharide composition purification instrument 有权
公开(公告)号：US20060121559A1
公开(公告)日：2006-06-08
申请号：US11061536
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12P21/06 , C12M1/34 , B01D15/08
CPC分类号： C12P19/00 , B01J20/3242 , B01J2220/603 , C12N11/00 , C12P21/005 , C12P21/06
摘要： The present invention provides a sugar chain purification instrument which can continuously and efficiently purify the sugar chain. According to the present invention, the instrument includes an immobilized protease column in which a carrier which can be packed in the column and on which protease is immobilized is packed and an immobilized glycopeptidase column in which a carrier which can be packed in the column and on which glycopeptidase is immobilized is packed, these columns being connected in series, and the reaction is carried out while continuously passing a glycoprotein to be decomposed together with a suitable buffer solution through the above columns. The step of liberation of sugar chain from the glycoprotein is highly simplified and labor-saving, and liberation of the sugar chain can be moderately and simply performed.

3. 发明授权

US07883873B2 Saccharide composition synthesizer 有权
标题翻译：糖组成合成仪
公开(公告)号：US07883873B2
公开(公告)日：2011-02-08
申请号：US11060810
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12P19/18 , C12P19/16 , B01J19/00
CPC分类号： C12P19/18
摘要： The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.
摘要翻译：本发明提供了当多个糖链连续反应时能够连续反应糖链的糖链合成器。本发明的糖链合成装置包括含有各糖苷溶液的多个容器，含有各糖基转移酶的多个容器，以及含有水溶性聚合物的底物的反应器，上述糖核苷酸溶液和糖基转移酶。在本发明中，通过超滤塔将反应器中得到的反应溶液中的成分分离，然后将反应产物返回到上述反应器中，以连续合成糖链。糖链的合成虽然复杂，但可以连续自动进行合成。

4. 发明申请

US20050221472A1 Saccharide composition synthesizer 有权
标题翻译：糖组成合成仪
公开(公告)号：US20050221472A1
公开(公告)日：2005-10-06
申请号：US11060810
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12P19/18 , B01J8/04 , C12M1/34 , C12M1/40
CPC分类号： C12P19/18
摘要： The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.
摘要翻译：本发明提供了当多个糖链连续反应时能够连续反应糖链的糖链合成器。本发明的糖链合成装置包括含有各糖苷溶液的多个容器，含有各糖基转移酶的多个容器，以及含有水溶性聚合物的底物的反应器，上述糖核苷酸溶液和糖基转移酶。在本发明中，通过超滤塔将反应器中得到的反应溶液中的成分分离，然后将反应产物返回到上述反应器，以连续合成糖链。糖链的合成虽然复杂，但可以连续自动进行合成。

5. 发明授权

US06962809B1 Sugar chain synthesizer 失效
标题翻译：糖链合成器
公开(公告)号：US06962809B1
公开(公告)日：2005-11-08
申请号：US10482626
申请日：2002-07-01
申请人： Kisaburo Deguchi , Genzo Hirata , Junkichi Miura , Masahito Ito , Shinichiro Nishimura , Susumu Nishiguchi , Atsushi Toda , Hiroaki Nakagawa , Kuriko Yamada
发明人： Kisaburo Deguchi , Genzo Hirata , Junkichi Miura , Masahito Ito , Shinichiro Nishimura , Susumu Nishiguchi , Atsushi Toda , Hiroaki Nakagawa , Kuriko Yamada
IPC分类号： C12P19/14 , C12P19/18 , C12M1/34
CPC分类号： C12P19/18 , C12P19/14
摘要： A sugar chain synthesizer comprising one or more reaction columns packed with immobilized glycosyltransferase and/or glycosidase; one or more separation means, arranged downstream from the reaction columns, for separating reaction products, unreaction products and byproducts contained in the solution eluted from the reaction columns; a first pump for feeding a primer of water soluble polymer and buffer solution to the reaction columns through a first selector valve; a second pump for feeding a buffer solution and sugar nucleotide solution to any one of the reaction columns through a second selector valve; one or more circulation flow paths connecting between a flow path downstream from the separation means and a flow path upstream from each of the reaction columns; and a third selector valve, arranged between the separation means and one or more circulation flow paths, for selective connection between the separation means and a desired circulation flow path. This synthesizer ensures continuous and automatic synthesis of sugar chains even if complicated.
摘要翻译：一种糖链合成仪，其包含一个或多个填充有固定的糖基转移酶和/或糖苷酶的反应柱; 一个或多个分离装置，布置在反应塔的下游，用于分离从反应塔洗脱的溶液中所含的反应产物，未反应产物和副产物; 第一泵，用于通过第一选择阀向反应塔供给水溶性聚合物和缓冲溶液的底漆; 第二泵，其通过第二选择阀将缓冲溶液和糖核苷酸溶液供给到任一反应塔; 连接在分离装置下游的流动路径和每个反应塔上游的流动路径之间的一个或多个循环流动路径; 以及第三选择阀，布置在所述分离装置和一个或多个循环流动路径之间，用于分离装置和期望的循环流动路径之间的选择性连接。这种合成器即使复杂，也能确保糖链的连续和自动合成。

6. 发明授权

US08232079B2 Oligosaccharide synthesizer 失效
标题翻译：寡糖合成仪
公开(公告)号：US08232079B2
公开(公告)日：2012-07-31
申请号：US11197335
申请日：2005-08-05
申请人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12M1/00 , C12P19/18
CPC分类号： C12P19/30
摘要： After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.
摘要翻译：在各种糖核苷酸溶液和糖基转移酶（或引物）已被混合之后，将它们引入固定在其上的引物（或糖基转移酶）的反应罐（柱）中。然后将从反应槽出来的溶液引入超滤柱。根据本发明的寡糖合成仪配备有用于确保由超滤塔分离的糖基转移酶或引物返回到用于将每种溶液存储在样品注射器中的容器中的流动路径。

7. 发明授权

US07070988B2 Oligosaccharide synthesizer 失效
公开(公告)号：US07070988B2
公开(公告)日：2006-07-04
申请号：US10715385
申请日：2003-11-19
申请人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12M1/34
CPC分类号： C12P19/30
摘要： After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.

8. 发明申请

US20060024817A1 Oligosaccharide synthesizer 失效
标题翻译：寡糖合成仪
公开(公告)号：US20060024817A1
公开(公告)日：2006-02-02
申请号：US11197335
申请日：2005-08-05
申请人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12M1/34 , C40B60/14
CPC分类号： C12P19/30
摘要： After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.
摘要翻译：在各种糖核苷酸溶液和糖基转移酶（或引物）已被混合之后，将它们引入固定在其上的引物（或糖基转移酶）的反应罐（柱）中。然后将从反应槽出来的溶液引入超滤柱。根据本发明的寡糖合成仪配备有用于确保由超滤塔分离的糖基转移酶或引物返回到用于将每种溶液存储在样品注射器中的容器中的流动路径。

9. 发明授权

US09139607B2 Method of purifying/concentrating sugar chain with sugar chain-trapping molecule and method of analyzing sugar chain structure 有权
标题翻译：用糖链捕获分子纯化/浓缩糖链的方法和糖链结构分析方法
公开(公告)号：US09139607B2
公开(公告)日：2015-09-22
申请号：US10540723
申请日：2003-12-25
申请人： Shinichiro Nishimura , Kenichi Niikura , Hiroaki Nakagawa , Minenobu Okayama
发明人： Shinichiro Nishimura , Kenichi Niikura , Hiroaki Nakagawa , Minenobu Okayama
IPC分类号： C07H1/08 , C07C239/20
CPC分类号： C07H1/08 , C07C239/20 , C07H1/06 , C07H3/06 , C07K1/14 , C07K14/77 , C07K14/79 , C07K16/00 , G01N33/5308 , G01N33/6842 , G01N2333/77 , G01N2333/79 , G01N2400/00 , G01N2560/00
摘要： The present invention provides substance which can specifically interact with sugar chains. Further, the present invention provides a method for separating, concentrating, or purifying sugar chains or a sugar chain-containing substance in a sample, comprising the steps of: a) contacting a sugar chain-trapping carrier comprising a substance which can specifically interact with sugar chains with the sample in a fluid phase under conditions that the sugar chain-trapping carrier can react with the sugar chains or sugar chain-containing substance; b) isolating a composite of the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance from the fluid phase; and c) exposing the composite to the conditions that the interaction between the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance is at least partially eliminated.
摘要翻译：本发明提供可以与糖链特异性相互作用的物质。此外，本发明提供了一种用于在样品中分离，浓缩或纯化糖链或含糖链物质的方法，包括以下步骤：a）使含糖链捕获载体与包含可特异性相互作用的物质在链条捕获载体可以与糖链或含糖链物质反应的条件下，样品在液相中的糖链; b）从流体相中分离捕获糖链的载体和糖链或含糖链的物质的复合物; 以及c）将所述复合材料暴露于所述糖链捕获载体和所述糖链或含糖链物质之间的相互作用被至少部分消除的条件。

10. 发明申请

US20060188996A1 Method of purifying/concentrating sugar chain with sugar chain-trapping molecule and mehtod of analyzing sugar chain structure 有权
标题翻译：用糖链捕获分子纯化/浓缩糖链的方法和分析糖链结构的方法
公开(公告)号：US20060188996A1
公开(公告)日：2006-08-24
申请号：US10540723
申请日：2003-12-25
申请人： Shinichiro Nishimura , Kenichi Niikura , Hiroaki Nakagawa , Minenobu Okayama
发明人： Shinichiro Nishimura , Kenichi Niikura , Hiroaki Nakagawa , Minenobu Okayama
IPC分类号： G01N33/00 , C08B37/00
CPC分类号： C07H1/08 , C07C239/20 , C07H1/06 , C07H3/06 , C07K1/14 , C07K14/77 , C07K14/79 , C07K16/00 , G01N33/5308 , G01N33/6842 , G01N2333/77 , G01N2333/79 , G01N2400/00 , G01N2560/00
摘要： The present invention provides substance which can specifically interact with sugar chains. Further, the present invention provides a method for separating, concentrating, or purifying sugar chains or a sugar chain-containing substance in a sample, comprising the steps of: a) contacting a sugar chain-trapping carrier comprising a substance which can specifically interact with sugar chains with the sample in a fluid phase under conditions that the sugar chain-trapping carrier can react with the sugar chains or sugar chain-containing substance; b) isolating a composite of the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance from the fluid phase; and c) exposing the composite to the conditions that the interaction between the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance is at least partially eliminated.
摘要翻译：本发明提供可以与糖链特异性相互作用的物质。此外，本发明提供了一种用于在样品中分离，浓缩或纯化糖链或含糖链物质的方法，包括以下步骤：a）使含糖链捕获载体与包含可特异性相互作用的物质在链条捕获载体可以与糖链或含糖链物质反应的条件下，样品在液相中的糖链; b）从流体相中分离捕获糖链的载体和糖链或含糖链的物质的复合物; 以及c）将所述复合材料暴露于所述糖链捕获载体和所述糖链或含糖链物质之间的相互作用被至少部分消除的条件。

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