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    • 5. 发明授权
    • Method and apparatus for disrupting cells and amplifying nucleic acids using gold nanorods
    • 使用金纳米棒破坏细胞和扩增核酸的方法和装置
    • US08557568B2
    • 2013-10-15
    • US13107677
    • 2011-05-13
    • Kwang Ho CheongDong-kee YiJeong-gun LeeJong-myeon Park
    • Kwang Ho CheongDong-kee YiJeong-gun LeeJong-myeon Park
    • C12M1/34C12M3/00C12M1/00
    • C12N1/066C12M47/06C12N13/00C12P19/34
    • Provided is a method of disrupting cells comprising adding gold nanorods to a solution containing cells and irradiating the gold nanorods with a laser to disrupt the cells. A method and an apparatus for continuously disrupting cells and amplifying nucleic acids in a single microchamber are also provided, wherein the method comprises introducing a solution containing cells and gold nanorods into a microchamber, irradiating a laser onto the gold nanorods to disrupt the cells, and amplifying a nucleic acid from the disrupted cells in the microchamber. The apparatus comprises a cell disruption chamber comprising a sample inlet, and gold nanorods introduced therein; a laser attached to the cell disruption chamber, wherein the laser is for generating light at a wavelength absorbed by the gold nanorods; and a heater and a cooler for heating and cooling the cell disruption chamber. Also disclosed is a lab-on-a chip comprising the apparatus.
    • 提供一种破坏细胞的方法,包括向含有细胞的溶液中加入金纳米棒并用激光照射金纳米棒以破坏细胞。 还提供了用于在单个微室中连续破坏细胞和扩增核酸的方法和装置,其中所述方法包括将含有细胞和金纳米棒的溶液引入微室中,将激光照射到金纳米棒上以破坏细胞,以及 在微室中从破碎的细胞中扩增核酸。 该装置包括一个细胞破碎室,包括一个样品入口和引入其中的金纳米棒; 附着到细胞破碎室的激光器,其中所述激光器用于产生由所述金纳米棒吸收的波长的光; 以及用于加热和冷却细胞破碎室的加热器和冷却器。 还公开了一种包括该装置的芯片实验室。