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    • 4. 发明授权
    • Method for separation and recovery of biological materials
    • 生物材料的分离和回收方法
    • US5552325A
    • 1996-09-03
    • US916801
    • 1992-07-17
    • Samuel NochumsonBruce S. Goldberg
    • Samuel NochumsonBruce S. Goldberg
    • G01N1/10B01L3/14G01N33/49G01N33/543G01N1/18
    • B01L3/5021G01N33/491G01N33/54366Y10T436/25375Y10T436/255
    • Disclosed is a centrifuge tube containing a porous selection means for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, the tube comprising separate upper and lower containers and means for joining the two containers to form a unitary device, the bottom of the upper container comprising a porous selection means, preferably a membrane containing a particulate substance which will selectively bind the biological substances while allowing unbound substances to pass through from the upper to lower container while being centrifuged. A method for recovering the biological substances in high concentration using the centrifuge tube is also provided, comprising introducing said gels, broths, or solutions containing the desired biological substances, centrifuging the same until the desired substance is bound to the selection means, and eluting the bound material from the selection means to recover the substance in concentrated form.
    • 公开了一种离心管,其包含多孔选择装置,用于从凝胶,肉汤或含有它们的溶液中选择性分离和回收生物物质,并因此浓缩它们,所述管包括分开的上部和下部容器以及用于连接两个容器以形成 上部容器的底部包括多孔选择装置,优选是含有颗粒物质的膜,该颗粒物质将选择性地结合生物物质同时允许未结合的物质在离心时从上到下容器通过。 还提供了使用离心管回收高浓度生物物质的方法,包括引入所述凝胶,肉汤或含有所需生物物质的溶液,将其离心,直到所需物质与选择装置结合, 来自选择的结合材料意味着以浓缩形式回收物质。