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1. 发明授权

US07265213B2 Methodology of conjugating chelators to biomolecules 有权
标题翻译：将螯合剂与生物分子结合的方法
公开(公告)号：US07265213B2
公开(公告)日：2007-09-04
申请号：US11191001
申请日：2005-07-28
申请人： Mekbib Astatke , Gordana Pajkovic , Danielle Lynee Russell
发明人： Mekbib Astatke , Gordana Pajkovic , Danielle Lynee Russell
IPC分类号： C07K1/10 , C01B21/16 , C07D213/00 , G01N33/532 , G01N33/20
CPC分类号： C07D213/82 , C07C235/84
摘要： A novel method of conjugating chelators to biomolecules such as proteins is provided. More particularly, the invention provides compositions and methods of using those compositions for the detection, purification and transport of divalent metal cation binding biomolecules.
摘要翻译：提供了一种将螯合剂与生物分子如蛋白质结合的新方法。更具体地，本发明提供了使用这些组合物来检测，纯化和转运二价金属阳离子结合生物分子的组合物和方法。

2. 发明授权

US08043816B2 Compositions and methods for temperature-dependent nucleic acid synthesis 有权
标题翻译：用于温度依赖性核酸合成的组合物和方法
公开(公告)号：US08043816B2
公开(公告)日：2011-10-25
申请号：US12468036
申请日：2009-05-18
申请人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

3. 发明申请

US20100233699A1 PRIMERS AND METHODS FOR THE DETECTION AND DISCRIMINATION OF NUCLEIC ACIDS 有权
标题翻译：用于检测和鉴别核酸的原理和方法
公开(公告)号：US20100233699A1
公开(公告)日：2010-09-16
申请号：US12629045
申请日：2009-12-01
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
摘要翻译：本发明提供用于检测特异性核酸序列的新型引物和方法。本发明的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。

4. 发明授权

US08394609B2 Primers and methods for the detection and discrimination of nucleic acids 有权
公开(公告)号：US08394609B2
公开(公告)日：2013-03-12
申请号：US12629045
申请日：2009-12-01
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C12P19/34
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

5. 发明申请

US20050123932A1 Nucleic acid-chelating agent conjugates 审中-公开
标题翻译：核酸螯合剂缀合物
公开(公告)号：US20050123932A1
公开(公告)日：2005-06-09
申请号：US10729898
申请日：2003-12-09
申请人： Mekbib Astatke
发明人： Mekbib Astatke
IPC分类号： C12N9/38 , C12P19/34 , C12Q1/68 , C07H21/04 , C07K14/00
CPC分类号： C12P19/34 , C12Q1/6806 , C12Q2563/137 , C12Q2523/101 , C12Q2521/101
摘要： A nucleotide having covalently bonded thereto a chelating agent can be used by a nucleic acid polymerase to synthesize a nucleic acid-chelating agent conjugate. The nucleic acid-chelating agent conjugate can chelate a transition metal ion and be used to detect a polyhistidine-containing recombinant protein.
摘要翻译：共价键合有螯合剂的核苷酸可以被核酸聚合酶用于合成核酸螯合剂缀合物。核酸螯合剂缀合物可螯合过渡金属离子并用于检测含多组氨酸的重组蛋白。

6. 发明申请

US20090275100A1 COMPOSITIONS AND METHODS FOR ENHANCED SENSITIVITY AND SPECIFICITY OF NUCLEIC ACID SYNTHESIS 有权
标题翻译：增强敏感性和核酸合成特异性的组合物和方法
公开(公告)号：US20090275100A1
公开(公告)日：2009-11-05
申请号：US12468036
申请日：2009-05-18
申请人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： C12N9/99
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

7. 发明申请

US20070178489A1 Compositions and Methods for Enhanced Sensitivity and Specificity of Nucleic Acid Synthesis 审中-公开
标题翻译：增强的核酸合成灵敏度和特异性的组合物和方法
公开(公告)号：US20070178489A1
公开(公告)日：2007-08-02
申请号：US11539347
申请日：2006-10-06
申请人： Mekbib Astatke , Deb Chatterjee , Harini Shadilya
发明人： Mekbib Astatke , Deb Chatterjee , Harini Shadilya
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/1252 , C12Y207/07007
摘要： The present invention relates to polypeptides, compositions and methods for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses polypeptides having affinity for double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) for use in such enhanced synthesis and more particularly to polymerases having reduced polymerase and optionally reduced exonuclease activities (3′ to 5′ and/or 5′ to 3′ exonuclease activity), and to nucleases having reduced nuclease activity. The polypeptides of the invention are capable of inhibiting nonspecific nucleic acid synthesis at ambient temperature. Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents non-specific nucleic acid synthesis at low temperatures, for example during reaction set up. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the polypeptides or compositions of the invention. The invention also relates to compositions prepared for carrying out the methods of the invention and to compositions made after or during such methods. The invention also generally relates to polypeptides and compositions useful for inhibiting or preventing degradation of various nucleic acid molecules.
摘要翻译：本发明涉及用于增强核酸分子合成的多肽，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体地，本发明公开了对双链和/或单链核酸分子和/或单链/双链核酸复合物（例如，引物/模板复合物，双链模板，单链/ 双链模板或单链引物），更具体地涉及具有降低的聚合酶和任选地减少的核酸外切酶活性（3'至5'和/或5'至3'外切核酸酶活性）的聚合酶，以及具有降低的核酸酶核酸酶活性。本发明的多肽能够在环境温度下抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止在低温下的非特异性核酸合成，例如在反应设置期间。本发明还涉及用于合成，扩增，逆转录或测序包含一种或多种本发明多肽或组合物的核酸分子的试剂盒。本发明还涉及制备用于实施本发明方法的组合物以及在这些方法之后或期间制备的组合物。本发明还通常涉及可用于抑制或预防各种核酸分子降解的多肽和组合物。

8. 发明申请

US20070026535A1 Novel methodology of conjugating chelators to biomolecules 有权
标题翻译：将螯合剂与生物分子结合的新方法
公开(公告)号：US20070026535A1
公开(公告)日：2007-02-01
申请号：US11191001
申请日：2005-07-28
申请人： Mekbib Astatke , Gordana Pajkovic , Danielle Russell
发明人： Mekbib Astatke , Gordana Pajkovic , Danielle Russell
IPC分类号： G01N33/53 , C07D213/46
CPC分类号： C07D213/82 , C07C235/84
摘要： A novel method of conjugating chelators to biomolecules such as proteins is provided. More particularly, the invention provides compositions and methods of using those compositions for the detection, purification and transport of divalent metal cation binding biomolecules.
摘要翻译：提供了一种将螯合剂与生物分子如蛋白质结合的新方法。更具体地，本发明提供了使用这些组合物来检测，纯化和转运二价金属阳离子结合生物分子的组合物和方法。

9. 发明申请

US20050089922A1 Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis 审中-公开
标题翻译：增强核酸合成灵敏度和特异性的组合物和方法
公开(公告)号：US20050089922A1
公开(公告)日：2005-04-28
申请号：US11007184
申请日：2004-12-09
申请人： Mekbib Astatke , Deb Chatterjee , Gary Gerard
发明人： Mekbib Astatke , Deb Chatterjee , Gary Gerard
IPC分类号： A61K48/00 , C12N9/22 , C12P19/34 , C12Q1/68 , G01N33/53
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

10. 发明申请

US20110160289A1 COMPOSITIONS AND METHODS FOR ENHANCED SENSITIVITY AND SPECIFICITY OF NUCLEIC ACID SYNTHESIS 审中-公开
标题翻译：增强敏感性和核酸合成特异性的组合物和方法
公开(公告)号：US20110160289A1
公开(公告)日：2011-06-30
申请号：US12772961
申请日：2010-05-03
申请人： Mekbib ASTATKE , Deb K. Chatterjee , Gary F. Gerard
发明人： Mekbib ASTATKE , Deb K. Chatterjee , Gary F. Gerard
IPC分类号： A61K31/7052 , C07H21/00 , C12N9/12 , C12P19/34 , C12Q1/68 , C12N9/99 , A61P31/12
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

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