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1. 发明授权

US11078525B2 Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage 有权
公开(公告)号：US11078525B2
公开(公告)日：2021-08-03
申请号：US14008096
申请日：2012-03-29
申请人： Jong Yoon Chun , Young Jo Lee
发明人： Jong Yoon Chun , Young Jo Lee
IPC分类号： C12P19/34 , C12Q1/6823 , C12Q1/6834 , C12Q1/6853
摘要： The present invention relates to the detection of a target nucleic acid sequence by a PCEC (PTO Cleavage and Extension-Dependent Cleavage) assay. The present invention is characterized by generating a cleavage site for a nucleolytic enzyme on the extended duplex of which the formation is dependent on the presence of a target nucleic acid sequence. The present invention detects the occurrence of the cleavage of the extended duplex, thereby determining the presence of the target nucleic acid sequence.

2. 发明授权

US08323895B2 Processes using dual specificity oligonucleotide and dual specificity oligonucleotide 有权
公开(公告)号：US08323895B2
公开(公告)日：2012-12-04
申请号：US13329667
申请日：2011-12-19
申请人： Jong-Yoon Chun
发明人： Jong-Yoon Chun
IPC分类号： C12Q1/68
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/6848 , C12Q1/6853 , C12Q1/6858 , C12Q2527/107 , C12Q2525/117 , C12Q2525/161 , C12Q2535/125
摘要： The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.

3. 发明申请

US20120164692A1 ANNEALING CONTROL PRIMER AND ITS USES 有权
标题翻译：退火控制器及其使用
公开(公告)号：US20120164692A1
公开(公告)日：2012-06-28
申请号：US13402980
申请日：2012-02-23
申请人： Jong-Yoon CHUN
发明人： Jong-Yoon CHUN
IPC分类号： C12P19/34 , C07H1/00 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6853 , C12Q2525/101
摘要： The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3′-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5′-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3′-end portion and said 5′-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.
摘要翻译：本发明涉及用于提高核酸扩增退火特异性的退火对照引物及其在核酸扩增涉及技术的各个领域的应用。本发明的引物包含（a）具有与模板核酸上的位点基本上互补的杂交核苷酸序列的3'端部分，以与其杂交; （b）具有预先选择的任意核苷酸序列的5'-端部分; 和（c）位于所述3'-末端部分和所述5'-末端部分之间的调节部分，其包含至少一个通用碱基或非歧视性碱基类似物，由此所述调节部分能够调节所述引物的退火部分与退火温度有关。

4. 发明授权

US08124346B2 Annealing control primer and its uses 有权
标题翻译：退火控制底漆及其用途
公开(公告)号：US08124346B2
公开(公告)日：2012-02-28
申请号：US12458702
申请日：2009-07-21
申请人： Jong-Yoon Chun
发明人： Jong-Yoon Chun
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6853 , C12Q2525/101
摘要： The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3′-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5′-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3′-end portion and said 5′-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.
摘要翻译：本发明涉及用于提高核酸扩增退火特异性的退火对照引物及其在核酸扩增涉及技术的各个领域的应用。本引物包括：（a）具有对模板核酸杂交与其部位基本上互补的杂交的核苷酸序列的3'末端部分; （b）具有一个预先选定的任意核苷酸序列的5'-末端部分; 和（c）位于所述3'-末端部分和所述5'-末端部分之间的调节部分，其包含至少一个通用碱基或非歧视性碱基类似物，由此所述调节部分能够调节所述引物的退火部分与退火温度有关。

5. 发明申请

US20080305478A1 Processes Using Dual Specificity Oligonucleotide and Dual Specificity Oligonucleotide 有权
标题翻译：双重特异性寡核苷酸和双重特异性寡核苷酸的过程
公开(公告)号：US20080305478A1
公开(公告)日：2008-12-11
申请号：US11817838
申请日：2006-03-03
申请人： Jong-Yoon Chun
发明人： Jong-Yoon Chun
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/6848 , C12Q1/6853 , C12Q1/6858 , C12Q2527/107 , C12Q2525/117 , C12Q2525/161 , C12Q2535/125
摘要： The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.
摘要翻译：本发明涉及通过使用双特异性寡核苷酸的模板依赖性延伸反应和由三种不同的Tm部分组成的双重特异性寡核苷酸的各种方法。在本发明中证明的是具有高杂交特异性和错配耐受性的双特异性寡核苷酸的特征。

6. 发明授权

US6063912A Placenta trophoblast-specific gene 失效
标题翻译：胎盘滋养细胞特异性基因
公开(公告)号：US6063912A
公开(公告)日：2000-05-16
申请号：US129888
申请日：1998-08-06
申请人： Jong-Yoon Chun , Yun-Jeong Han
发明人： Jong-Yoon Chun , Yun-Jeong Han
IPC分类号： C07K14/47 , C07H21/04
CPC分类号： C07K14/4702
摘要： Expression of the polynucleotide molecule, Psx, is restricted to placenta, and in particular, to placenta trophoblast cell layers during embryogenesis. The expression pattern of Psx is exploited to detect trophoblast specific lineages, such as labyrinthine trophoblast layer and giant cells. The invention provides an isolated DNA molecule encoding Psx protein, and chimeric constructs, vector and host cells containing the isolated DNA. Also provided is a method for identifying putative abortion-inducing agents, which may offer an alternative to surgical abortion.
摘要翻译：多核苷酸分子Psx的表达限于胎盘，特别是胚胎发生期间对胎盘滋养层细胞层的表达。 Psx的表达模式被用于检测滋养层特异性谱系，如迷路滋养层和巨细胞。本发明提供了编码Psx蛋白的分离的DNA分子和含有分离的DNA的嵌合构建体，载体和宿主细胞。还提供了用于鉴定推定的流产诱导剂的方法，其可以提供手术流产的替代方案。

7. 发明授权

US10552503B2 Content evaluation method and server in network environment 有权
公开(公告)号：US10552503B2
公开(公告)日：2020-02-04
申请号：US15532051
申请日：2015-12-04
申请人： Jong Yoon Chun
发明人： Sol Jee Chun
IPC分类号： G06F16/95 , G06F16/9535 , G06F16/48 , G06Q50/10 , G06Q10/00
摘要： The present invention relates to a content evaluation method and a content evaluation server in a network environment. The present invention compares a content tag of an applicant with an evaluator tag for evaluating contents such that contents may be evaluated by an evaluator in accordance with the content tag, ensuring a more reliable evaluation through content evaluation by suitable evaluators.

8. 发明授权

US09885081B2 Detection of target nucleic acid sequences using dual-labeled immobilized probes on solid phase 有权
公开(公告)号：US09885081B2
公开(公告)日：2018-02-06
申请号：US13880199
申请日：2011-10-20
申请人： Jong Yoon Chun
发明人： Jong Yoon Chun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6818 , C12Q1/6823 , C12Q1/6837 , C12Q2521/325 , C12Q2525/125 , C12Q2565/1015 , C12Q2521/319
摘要： The present invention relates to a novel method for detection of target nucleic acid sequences on a solid phase using dual-labeled immobilized probes and its resistance to a 5′ to 3′ exonuclease activity of a DNA polymerase. Because the label is remained on the solid substrate by resistance to nucleases due to labeling of a base component the internal nucleotide, the present invention requires no consideration of a suitability of position of the label for remaining on the solid substrate. The present invention ensures to minimize background signal by positioning labels at a site on probes suitable to maximize quenching efficiency of the dual label system, since it permits to freely determine the position of the internal label on probes.

9. 发明授权

US09884890B2 Process using dual specificity oligonucleotide and dual specificity oligonucleotide 有权
公开(公告)号：US09884890B2
公开(公告)日：2018-02-06
申请号：US13671621
申请日：2012-11-08
申请人： Jong-Yoon Chun
发明人： Jong-Yoon Chun
IPC分类号： C12Q1/68 , C07H21/04 , C07H21/02
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/6848 , C12Q1/6853 , C12Q1/6858 , C12Q2527/107 , C12Q2525/117 , C12Q2525/161 , C12Q2535/125
摘要： The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.

10. 发明授权

US09868980B2 Detection of target nucleic acid sequence by PTO cleavage and extension-dependent signaling oligonucleotide hybridization assay 有权
公开(公告)号：US09868980B2
公开(公告)日：2018-01-16
申请号：US14374567
申请日：2012-07-03
申请人： Jong Yoon Chun , Young Jo Lee
发明人： Jong Yoon Chun , Young Jo Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q2521/319 , C12Q2525/131 , C12Q2525/161 , C12Q2525/186 , C12Q2533/101 , C12Q2563/173 , C12Q2565/1015 , C12Q2565/514
摘要： The present invention relates to the detection of a target nucleic acid sequence by a PCE-SH (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Hybridization) assay. The present invention does not use probes to be hybridized with target nucleic acid sequences for providing target signals. Interestingly, the present invention uses probes (signaling oligonucleotides) to be hybridized with the extended strand formed in a target-dependent manner in which the extended strand is synthesized using the CTO artificially selected as templates.

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