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1. 发明授权

US08685682B2 Docosahexaenoic acid producing strains of yarrowia lipolytica 有权
标题翻译：二十二碳六烯酸生产的解脂耶氏酵母菌株
公开(公告)号：US08685682B2
公开(公告)日：2014-04-01
申请号：US12466072
申请日：2009-05-14
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K Picataggio , James John Ragghianti , John E Seip , Zhixiong Xue , Narendra S Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K Picataggio , James John Ragghianti , John E Seip , Zhixiong Xue , Narendra S Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： C12P7/64 , C12N1/00 , C12N9/02 , C12N15/00 , A23L1/28 , A23L1/30 , C07C57/02
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译：描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。

2. 发明申请

US20120171719A1 USE OF SACCHAROMYCES CEREVISIAE SUC2 GENE IN YARROWIA LIPOLYTICA FOR SUCROSE UTILIZATION 有权
标题翻译：在沙鼠中使用沙门氏菌SUC2基因进行枯草杆菌利用
公开(公告)号：US20120171719A1
公开(公告)日：2012-07-05
申请号：US13296524
申请日：2011-11-15
申请人： Seung-Pyo Hong , John E. Seip , Quinn Qun Zhu
发明人： Seung-Pyo Hong , John E. Seip , Quinn Qun Zhu
IPC分类号： C12P33/00 , C12P1/02 , C12P7/64 , C12P7/22 , C12P13/04 , C12P17/06 , C12P7/62 , C12P7/26 , C12N1/19 , C12P7/02
CPC分类号： C12N9/2431 , C07K2319/02 , C12N9/60 , C12P7/64 , C12Y302/01026
摘要： Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
摘要翻译：本文公开的是转化的解脂耶氏酵母病，其包含编码具有蔗糖转化酶活性的多肽的外源多核苷酸。还公开了使用转化的解脂耶氏酵母的方法。

3. 发明授权

US07923223B2 Δ-9 desaturase and its use in making polyunsaturated fatty acids 有权
标题翻译：＆Dgr; -9去饱和酶及其在制备多不饱和脂肪酸中的应用
公开(公告)号：US07923223B2
公开(公告)日：2011-04-12
申请号：US11613420
申请日：2006-12-20
申请人： John E. Seip , Quinn Qun Zhu
发明人： John E. Seip , Quinn Qun Zhu
IPC分类号： C12P7/64 , C12N9/00 , C12N9/02 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/6427 , C12N9/0083 , C12P7/6472 , C12Y114/19001
摘要： The present invention relates to a Δ9 desaturase, which has the ability to convert palmitic acid [16:0] or stearic acid [18:0] into palmitoleic acid [16:1] or oleic acid [18:1], respectively. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ9 desaturase in oleaginous yeast is disclosed.
摘要翻译：本发明涉及一种具有将棕榈酸[16：0]或硬脂酸[18：0]分别转化为棕榈油酸[16：1]或油酸[18：1]的能力的“去饱和酶” 。公开了分离的核酸片段和重组构建体，其包含编码＆Dgr。9去饱和酶的片段以及在含油酵母中使用该Dgr 9脱氢酶制备长链多不饱和脂肪酸（PUFA）的方法。

4. 发明授权

US07098005B2 Natural promoters for gene expression in C1 metabolizing bacteria 失效
公开(公告)号：US07098005B2
公开(公告)日：2006-08-29
申请号：US11251304
申请日：2005-10-14
申请人： Deana J. Dicosimo , Hao Ni , Stephen K. Picataggio , John E. Seip , Rick W. Ye , Tao Wang
发明人： Deana J. Dicosimo , Hao Ni , Stephen K. Picataggio , John E. Seip , Rick W. Ye , Tao Wang
IPC分类号： C12N1/21 , C07H21/04
CPC分类号： C12N15/74 , C07K14/195
摘要： Genes have been identified in the Methylomonas genome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e.g., crtZ) in the obligate methanotroph Methylomonas sp. 16a.

5. 发明授权

US08435758B2 Manipulation of SNF1 kinase for altered oil content in oleaginous organisms 有权
标题翻译：操纵SNF1激酶以改善含油生物体内油含量
公开(公告)号：US08435758B2
公开(公告)日：2013-05-07
申请号：US12549439
申请日：2009-08-28
申请人： Seung-Pyo Hong , Quinn Qun Zhu , John E. Seip
发明人： Seung-Pyo Hong , Quinn Qun Zhu , John E. Seip
IPC分类号： C12P1/00 , C07H21/04 , C12N1/15
CPC分类号： C12P7/6472 , C12P7/6427 , C12P7/6463
摘要： Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.
摘要翻译：公开了通过降低异源三聚SNF1蛋白激酶的活性来增加真核细胞中总脂质含量，多不饱和脂肪酸的总含量[“PUFA”]和/或去饱和脂肪酸与饱和脂肪酸的比例的方法。优选地，编码SNF1蛋白激酶的Snf1α-亚基，Gal83β-亚基或Snf4γ-亚基的染色体基因，编码Sak1，Hxk2，Glk1或Reg1的上游调控基因或编码Rme1，Cbr1或Snf3的下游基因与含有不具有降低的活性的异源三聚SNF1蛋白激酶的亲本菌株相比，在含油酵母解脂耶氏酵母解脂耶氏酵母解脂耶氏酵母的PUFA生产菌株中操作，导致总脂质含量增加。

6. 发明授权

US08735137B2 Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization 有权
标题翻译：在解脂耶氏酵母中使用酿酒酵母SUC2基因用于蔗糖利用
公开(公告)号：US08735137B2
公开(公告)日：2014-05-27
申请号：US13296524
申请日：2011-11-15
申请人： Seung-Pyo Hong , John E. Seip , Quinn Qun Zhu
发明人： Seung-Pyo Hong , John E. Seip , Quinn Qun Zhu
IPC分类号： C12N1/15 , C12N15/56 , C12N9/26 , C12N9/60
CPC分类号： C12N9/2431 , C07K2319/02 , C12N9/60 , C12P7/64 , C12Y302/01026
摘要： Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
摘要翻译：本文公开的是转化的解脂耶氏酵母病，其包含编码具有蔗糖转化酶活性的多肽的外源多核苷酸。还公开了使用转化的解脂耶氏酵母的方法。

7. 发明授权

US07550286B2 Docosahexaenoic acid producing strains of Yarrowia lipolytica 有权
标题翻译：二十二碳六烯酸生产的解脂耶氏酵母菌株
公开(公告)号：US07550286B2
公开(公告)日：2009-06-23
申请号：US11264737
申请日：2005-11-01
申请人： Howard Glenn Damude , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： C12N1/16 , C12P7/64 , C12N9/02 , C07H21/04
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译：描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。

8. 发明授权

US07026464B2 Natural promoters for gene expression in C1 metabolizing bacteria 失效
标题翻译： C1代谢细菌基因表达的天然启动子
公开(公告)号：US07026464B2
公开(公告)日：2006-04-11
申请号：US10689200
申请日：2003-10-20
申请人： Deana J. Dicosimo , Hao Ni , Stephen K. Picataggio , John E. Seip , Rick W. Ye , Tao Wang
发明人： Deana J. Dicosimo , Hao Ni , Stephen K. Picataggio , John E. Seip , Rick W. Ye , Tao Wang
IPC分类号： C07H21/04
CPC分类号： C12N15/74 , C07K14/195
摘要： Genes have been identified in the Methylomonas genome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e.g., crtZ) in the obligate methanotroph Methylomonas sp. 16a.
摘要翻译：已经在Methylomonas基因组中鉴定了对各种代谢和生长条件有反应的基因。这些基因的确定的反应性允许在C1代谢细菌中使用其启动子调节的基因表达。特别地，发现在其天然状态下驱动3-己糖-6-磷酸合成酶（HPS）表达的hps启动子可用于引导异源性编码区（例如，crtZ）在专性甲烷嗜甲基甲酸单体中表达 sp。 16a。

9. 发明申请

US20090311380A1 DOCOSAHEXAENOIC ACID PRODUCING STRAINS OF YARROWIA LIPOLYTICA 有权
标题翻译：青蒿素生产菌株的DOCOSAHEXAENOIC酸
公开(公告)号：US20090311380A1
公开(公告)日：2009-12-17
申请号：US12466072
申请日：2009-05-14
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： A23L1/30 , C12N1/19 , C07C57/03 , A23L1/28 , A23C9/12 , A23K1/16
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译：描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。

10. 发明申请

US20100062502A1 MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS 有权
标题翻译：用于改变OLE内含物的SNF1激酶的操作
公开(公告)号：US20100062502A1
公开(公告)日：2010-03-11
申请号：US12549439
申请日：2009-08-28
申请人： Seung-Pyo Hong , Quinn Qun Zhu , John E. Seip
发明人： Seung-Pyo Hong , Quinn Qun Zhu , John E. Seip
IPC分类号： C12P7/64 , C12N1/19 , C07C57/00 , C12N15/87
CPC分类号： C12P7/6472 , C12P7/6427 , C12P7/6463
摘要： Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.
摘要翻译：公开了通过降低异源三聚SNF1蛋白激酶的活性来增加真核细胞中总脂质含量，多不饱和脂肪酸的总含量[“PUFA”]和/或去饱和脂肪酸与饱和脂肪酸的比例的方法。优选地，编码SNF1蛋白激酶的Snf1α-亚基，Gal83＆bgr-亚基或Snf4γ-亚基的染色体基因，编码Sak1，Hxk2，Glk1或Reg1的上游调节基因，或编码Rme1，Cbr1或与含有不具有降低的活性的异源三聚SNF1蛋白激酶的亲本菌株相比，Snf3在含油酵母解脂耶氏酵母解脂耶氏酵母的PUFA产生菌株中被操纵，导致总脂质含量增加。

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