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1. 发明公开

KR1020140096632A 효소 반응법을 이용한 마이오-이노시톨로부터 D-카이로-이노시톨을 생산하는 방법 审中-实审
标题翻译：在体外酶反应中使用MYO-INOSITOL生产D-酪氨酸激酶的方法
公开(公告)号：KR1020140096632A
公开(公告)日：2014-08-06
申请号：KR1020130009431
申请日：2013-01-28
申请人： 주식회사 디와이내츄럴
发明人： 명현군 , 윤상활 , 이현서 , 류재하
IPC分类号： C12P7/02 , C12N9/02 , C12N9/90
CPC分类号： C12N9/90 , C12N9/0006 , C12P7/18 , C12Y101/01018 , C12Y503/99 , Y02P20/582
摘要： The present invention relates to a method for producing D-chiro-inositol by reaction of myo-inositol, as a substrate, in an enzymatic reaction buffer solution containing inositol dehydrogenase and inosose isomerase, or a fusion protein of the enzymes. D-chiro-inositol is produced in an extracellular enzymatic reaction buffer solution by the method of the present invention, thereby being able to produce the high yield D-chiro-inositol at low costs compared with a fermentation method using a recombinant cell. Also, the D-chiro-inositol can be isolated in a high purity from a mixed reaction buffer solution containing the myo-inositol and D-chiro-inositol by simple processes such as addition of an organic solvent, separation of a supernatant and drying. Additionally, according to the present invention, the myo-inositol separated from the D-chiro-inositol can be reused as a substrate.
摘要翻译：本发明涉及通过肌醇作为底物，在含有肌醇脱氢酶和肌糖异构酶的酶反应缓冲溶液或酶的融合蛋白中反应来制造D-手性肌醇的方法。通过本发明的方法在细胞外酶反应缓冲溶液中产生D-手性肌醇，从而与使用重组细胞的发酵方法相比，能够以低成本生产高产率D-手性肌醇。此外，通过简单的方法，例如添加有机溶剂，分离上清液和干燥，可以从含有肌醇和D-手性肌醇的混合反应缓冲溶液中以高纯度分离D-手性肌醇。此外，根据本发明，从D-手性肌醇分离的肌醇可以重新用作底物。

2. 发明公开

KR1020140078381A 휴면 세포 전환법을 이용한 Ｄ-카이로 이노시톨의 생산 방법 审中-实审
标题翻译：通过使用停留细胞转化来生产D-CHIRO-INOSITOL的方法
公开(公告)号：KR1020140078381A
公开(公告)日：2014-06-25
申请号：KR1020120147672
申请日：2012-12-17
申请人： 주식회사 디와이내츄럴
发明人： 명현군 , 윤상활 , 이현서
IPC分类号： C12P7/18 , C12P7/02 , C12N15/63 , C12N15/74
CPC分类号： C12Y503/99 , C12N9/0044 , C12P7/18 , C12Y101/01018
摘要： The present invention relates to a method for producing D-chiro-inositol from myo-inositol using a resting cell of a recombination cell which expresses a myo-inositol transporter, inositol dehydrogenases, and inosose-isomerase. According to the present invention, a minimum medium is used, repetitive use of the resting cell can be possible, and a conversion yield of the D-chiro-inositol from the myo-inositol is equal to that in a complete medium. Therefore, manufacturing costs can be dramatically reduced in the mass production of the D-chiro-inositol. Also, refinement efficiency of the D-chiro-inositol can be greatly improved by using the minimum medium instead of the complete medium.
摘要翻译：本发明涉及使用表达肌醇转运蛋白，肌醇脱氢酶和肌糖异构酶的重组细胞的静止细胞从肌醇制备D-手性肌醇的方法。根据本发明，使用最小的培养基，静息细胞的重复使用是可能的，并且肌醇中D-手性肌醇的转化产率等于完全培养基中的转化率。因此，在D-手性肌醇的批量生产中可以显着降低制造成本。此外，通过使用最小介质而不是完全介质，可以大大提高D-手性肌醇的精制效率。

3. 发明授权

KR101447579B1 미생물을 이용한 Ｄ-카이로 이노시톨의 생산 방법 有权
标题翻译：使用微生物制备D-双 - 肌醇的方法
公开(公告)号：KR101447579B1
公开(公告)日：2014-10-15
申请号：KR1020120107278
申请日：2012-09-26
申请人： 주식회사 디와이내츄럴
发明人： 명현군 , 윤상활 , 이현서
IPC分类号： C12N15/52 , C12N15/63 , C12N1/21 , C12P7/18
CPC分类号： C12P7/02 , C07K14/195 , C07K14/255 , C07K14/32 , C12N9/0006 , C12N9/90 , C12P7/18 , C12Y101/01018
摘要： 본 발명은 상기 효소 마이오-이노시톨 트랜스포터, 이노시톨 디하이드로게나아제 및 이노소스 이소머라아제를 발현하는 형질전환 숙주세포를 이용하여 마이오-이노시톨로부터 D-카이로-이노시톨을 생산하는 방법에 관한 것이다. 본 발명의 방법에 의하면 높은 수율로 마이오-이노시톨을 D-카이로-이노시톨로 전환시킬 수 있다.

4. 发明授权

KR101828714B1 중간 사슬 지방산 생산 촉진용 형질전환 대장균의 제조방법 및 이의 형질전환 대장균 有权
公开(公告)号：KR101828714B1
公开(公告)日：2018-02-13
申请号：KR1020160153119
申请日：2016-11-17
申请人： 울산과학기술원
发明人： 이성국 , 김창희
IPC分类号： C12N15/70 , C12N9/04 , C12N9/16 , C12P7/64
CPC分类号： C12N15/70 , C12N9/0006 , C12N9/16 , C12P7/6409 , C12Y101/01018 , C12Y301/02
摘要： 본발명은본 발명은, 중간사슬지방산생산촉진용형질전환대장균의제조방법에관한것으로서, (1) 야생형대장균의사이드패스(side pathway)에관여하는유전자들을넉-아웃(knock-out)시키는단계; (2) 상기대장균의중간사슬지방산을생산하는효소의유전자를유전자자리에치환하는단계; (3) 상기대장균의프로모터를프로모터로치환하는단계; 및 (4) 상기대장균의프로모터를프로모터로치환하는단계;를포함하는것이다.

5. 发明公开

KR1020140040509A 미생물을 이용한 Ｄ-카이로 이노시톨의 생산 방법 有权
标题翻译：通过使用微生物制备D-纤维蛋白酶的方法
公开(公告)号：KR1020140040509A
公开(公告)日：2014-04-03
申请号：KR1020120107278
申请日：2012-09-26
申请人： 주식회사 디와이내츄럴
发明人： 명현군 , 윤상활 , 이현서
IPC分类号： C12N15/52 , C12N15/63 , C12N1/21 , C12P7/18
CPC分类号： C12P7/02 , C07K14/195 , C07K14/255 , C07K14/32 , C12N9/0006 , C12N9/90 , C12P7/18 , C12Y101/01018
摘要： The present invention is a method for preparing D-chiro-inositol from myo-inositol by using a transformed host cell that expresses enzymes such as a myo-inositol transporter, inositol dehydrogenase, and inosose isomerase. According to the method of the present invention, myo-inositol can be converted into D-chiro-inositol at a high yield. [Reference numerals] (AA) D-chiro-inositol (g/L); (BB,DD) Time; (CC) Cell growth (OD at 600 nm); (EE) Inclination
摘要翻译：本发明是通过使用表达酶的转化宿主细胞，例如肌醇转运蛋白，肌醇脱氢酶和肌糖异构酶，由肌醇制备D-手性肌醇的方法。根据本发明的方法，可以高产率将肌醇转化为D-手性肌醇。（AA）D-手性肌醇（g / L）; （BB，DD）时间; （CC）细胞生长（600nm处的OD）; （EE）倾斜

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