专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

KR1020040079136A 인간 로타바이러스의 활성을 저해하는 항체의 생산용 항원및 그의 제조방법과 이용 无效
标题翻译：用于生产具有改善特异性的人类脊髓灰质炎抗体的抗原和抗原制备及其用途
公开(公告)号：KR1020040079136A
公开(公告)日：2004-09-14
申请号：KR1020030014070
申请日：2003-03-06
申请人： 주식회사 씨트리
发明人： 박희정 , 김승영 , 김순옥 , 장석구 , 김완주
IPC分类号： C12N15/70
CPC分类号： C07K14/005 , A23L33/10 , A23V2002/00 , A23V2200/324 , A61K35/57 , C07K16/10 , C07K2317/23 , C12N15/70 , C12N2720/12333
摘要： PURPOSE: An antigen and antigen preparation for the production of the antibody against human rotavirus, and the use thereof are provided, thereby mass-producing the antigen, and cheaply producing the antibody against human rotavirus having improved specificity from the egg yolk. CONSTITUTION: The antigen preparation for the production of the antibody against human rotavirus comprises the steps of: (a) RT-PCR amplifying the genomic RNA of human rotavirus as a template using a primer of SEQ ID NO:1 or SEQ ID NO:2 to selectively isolate a gene encoding VP8 antigen protein; (b) cloning the isolated gene into a vector, and transforming a microorganism with the vector to extract the DNA of the vector; (c) PCR amplifying the extracted vector DNA using primers of SEQ ID NO:3 and SEQ ID NO:4 to amplify the gene encoding the VP8 antigen protein; (d) cloning the amplified gene into an expression vector pET-29b and transforming Escherichia coli with the vector; and (e) culturing the transformed E. coli to over-express VP8 antigen protein and purifying the VP8 antigen protein by using the Ni+-NTA affinity column.
摘要翻译：目的：提供用于产生针对人轮状病毒的抗体的抗原和抗原制剂及其用途，从而批量生产抗原，并且廉价地生产具有改善的蛋黄特异性的抗轮状病毒抗体。构成：用于产生针对人轮状病毒的抗体的抗原制备方法包括以下步骤：（a）使用SEQ ID NO：1或SEQ ID NO：2的引物RT-PCR扩增人轮状病毒的基因组RNA作为模板选择性分离编码VP8抗原蛋白的基因; （b）将分离的基因克隆到载体中，并用载体转化微生物以提取载体的DNA; （c）使用SEQ ID NO：3和SEQ ID NO：4的引物PCR扩增提取的载体DNA以扩增编码VP8抗原蛋白的基因; （d）将扩增的基因克隆到表达载体pET-29b中并用载体转化大肠杆菌; 和（e）培养转化的大肠杆菌以过表达VP8抗原蛋白，并使用Ni + -NTA亲和柱纯化VP8抗原蛋白。

IPRDB

热门服务

关于我们

友情链接

联系方式