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    • 2. 发明公开
    • 렙틴을 이용한 줄기세포 증식방법
    • 用LEPTIN促进干细胞的方法
    • KR1020080042761A
    • 2008-05-15
    • KR1020070115056
    • 2007-11-12
    • 문찬일
    • 문찬일양현원
    • C12N5/07C12N5/00
    • C12N5/0647C12N2501/355
    • A method for proliferating stem cells is provided to proliferate a large quantity of stem cells without increasing the number of sub-culturing by adding leptin into the stem cells, so that change of stem cells in sub-culturing is inhibited and stability and economical efficiency of stem cell cultivation are improved. A stem cell is proliferated by culturing the stem cell in a serum or serum-free medium for cell culture containing leptin, its analogues or derivatives and a growth factor selected from erythropoietin, G-CSF(granulocyte-colony stimulating factor), GM-CSF(granulocyte macrophage-colony stimulating factor), LIF(leukemia inhibitory factor) and IL-3(interleukin-3), wherein the stem cell is bone marrow stem cell, bone marrow hematopoietic stem cell, stem cell in blood, hematopoietic stem cell in blood, placental blood stem cell, fetal stem cell or adipocyte stem cell. Further, the stem cell is be derived from human being or mouse.
    • 提供增殖干细胞的方法,通过向干细胞中加入瘦蛋白而不增加培养次数,从而增加干细胞的数量,从而抑制亚培养中干细胞的变化,稳定性和经济效率 干细胞培养得到改善。 通过在血清或无血清培养基中培养含有瘦蛋白,其类似物或衍生物的细胞培养物和选自促红细胞生成素,G-CSF(粒细胞集落刺激因子),GM-CSF的生长因子的干细胞来增殖干细胞 (粒细胞巨噬细胞集落刺激因子),LIF(白血病抑制因子)和IL-3(白介素-3),其中干细胞是骨髓干细胞,骨髓造血干细胞,血液中的干细胞,造血干细胞 血液,胎盘血干细胞,胎儿干细胞或脂肪细胞干细胞。 此外,干细胞来自人或小鼠。