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    • 7. 发明公开
    • 세포 또는 바이러스의 농축 및 용해용 미세유동장치 및방법
    • 微流体装置和细胞或病毒的浓度和分析方法
    • KR1020070063189A
    • 2007-06-19
    • KR1020050123161
    • 2005-12-14
    • 삼성전자주식회사
    • 조윤경이정건정성영
    • C12N13/00
    • B01L3/50273B01L3/502746B01L3/502761B01L2200/10B01L2300/1861B01L2400/0421B01L2400/0424B01L2400/043B01L2400/0439C12M47/02C12M47/04C12M47/06Y10T436/25Y10T436/2575
    • A microfluidic device and method for concentration and lysis of cells or viruses are provided to improve efficiency of cell concentration and lysis processes, and perform the concentration and lysis processes in one chamber, so that a lab-on-a-chip is miniaturized by using the same device and method. The microfluidic device for concentration and lysis of cells or viruses comprises: a magnetic bead(102); a reaction chamber(202) receiving the magnetic bead and having a dielectrically separated crossing electrode portion capable of generating the electric field, a fluid inlet(204), and a fluid outlet(206); a vibration portion(302) for agitating the magnetic bead in the chamber; and a laser-generating portion(402) for supplying laser(404) to the magnetic bead in the chamber, wherein the fluid contains the magnetic bead or cells or viruses(502); and the size of the magnetic bead is 50 nm-1000 mum, and contains Fe, Ni, Cr or their oxide. The method for concentration and lysis of cells or viruses comprises the steps of: applying the voltage to the crossing electrode portion to generate spatially ununiformed electric field in the chamber; introducing the fluid containing cells or viruses into the reaction chamber; introducing the fluid containing the magnetic bead into the reaction chamber; and irradiating the laser to the magnetic bead.
    • 提供用于细胞或病毒的浓缩和裂解的微流体装置和方法,以提高细胞浓度和裂解过程的效率,并在一个室中进行浓缩和裂解过程,从而通过使用 相同的设备和方法。 用于细胞或病毒浓缩和裂解的微流体装置包括:磁珠(102); 接收磁珠并具有能够产生电场的介电分离的交叉电极部分的反应室(202),流体入口(204)和流体出口(206); 用于搅拌所述腔室中的磁珠的振动部分(302); 以及用于将激光器(404)供应到所述腔室中的磁珠的激光产生部分(402),其中所述流体包含所述磁珠或细胞或病毒(502); 磁珠的大小为50nm〜1000μm,含有Fe,Ni,Cr或其氧化物。 细胞或病毒的浓缩和裂解方法包括以下步骤:将电压施加到交叉电极部分以在腔室中产生空间不均匀的电场; 将含有细胞或病毒的流体引入反应室; 将含有磁珠的流体引入反应室; 并将激光照射到磁珠上。