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1. 发明公开

KR1020040034278A ＨＩＶ 뉴클레오캡시드 단백질의 동물세포 발현용 벡터 및 이의 제조방법 有权
标题翻译：在哺乳动物细胞中制备艾滋病毒的核酸（NC）蛋白的方法
公开(公告)号：KR1020040034278A
公开(公告)日：2004-04-28
申请号：KR1020020064380
申请日：2002-10-21
申请人： 유지창 , (주) 에빅스젠
发明人： 유지창
IPC分类号： C07K14/435
CPC分类号： C07K14/005 , C12N15/85 , C12N2740/10034 , C12N2740/10051
摘要： PURPOSE: Provided is a process for preparing a nucleocapsid(NC) protein of HIV in a mammalian cell easily. The nucleocapsid(NC) protein of HIV can be useful for development of anti-viral agent for treating HIV. CONSTITUTION: A process for preparing a nucleocapsid(NC) protein of HIV in a mammalian cell comprises the steps of: constructing an expression vector containing the HIV nucleocapsid protein gene, SV40 19s mRNA intron sequence or modified SV40 16s mRNA intron sequence positioned at the upstream of the NC gene, and CMV promoter; transforming the animal cell with the expression vector; culturing the transformed animal cell; and recovering the nucleocapsid(NC) protein of HIV, wherein the animal cell is COS-7 or 293T cell.
摘要翻译：目的：提供一种在哺乳动物细胞中容易制备HIV的核衣壳（NC）蛋白的方法。 HIV的核衣壳（NC）蛋白质可用于开发用于治疗HIV的抗病毒剂。构成：在哺乳动物细胞中制备HIV的核衣壳（NC）蛋白质的方法包括以下步骤：构建含有HIV核衣壳蛋白基因，SV40,19s mRNA内含子序列或位于上游的修饰的SV4016s mRNA内含子序列的表达载体的NC基因和CMV启动子; 用表达载体转化动物细胞; 培养转化的动物细胞; 并回收HIV的核衣壳（NC）蛋白，其中动物细胞是COS-7或293T细胞。

2. 发明公开

KR1020030082191A ＨＩＶ-1의 누클레오캡시드 단백질과 결합할 수 있는 단백질 有权
标题翻译：蛋白质与HIV-1的核酸（NC）蛋白结合
公开(公告)号：KR1020030082191A
公开(公告)日：2003-10-22
申请号：KR1020020020805
申请日：2002-04-17
申请人： 유지창 , 명희준
发明人： 유지창 , 명희준
IPC分类号： C07K14/16
CPC分类号： C07K14/005 , C12N2740/16011
摘要： PURPOSE: Proteins for binding to nucleocapsid(NC) protein of HIV-1 are provided, thereby effectively inhibiting the growth of HIV-1. CONSTITUTION: A peptide pep A having the amino acid sequence of SEQ ID NO: 1 specifically binds to nucleocapsid(NC) protein of HIV-1. A peptide pep B having the amino acid sequence of SEQ ID NO: 2 specifically binds to nucleocapsid(NC) protein of HIV-1. A peptide pep C having the amino acid sequence of SEQ ID NO: 3 specifically binds to nucleocapsid(NC) protein of HIV-1. A peptide pep 1 having the amino acid sequence of SEQ ID NO: 11 specifically binds to nucleocapsid(NC) protein of HIV-1. A peptide pep 2 having the amino acid sequence of SEQ ID NO: 12 specifically binds to nucleocapsid(NC) protein of HIV-1. A peptide pep 3 having the amino acid sequence of SEQ ID NO: 13 specifically binds to nucleocapsid(NC) protein of HIV-1.
摘要翻译：目的：提供与HIV-1核衣壳（NC）蛋白结合的蛋白质，从而有效抑制HIV-1的生长。构成：具有SEQ ID NO：1的氨基酸序列的肽肽A特异性结合HIV-1的核衣壳（NC）蛋白。具有SEQ ID NO：2的氨基酸序列的肽肽B特异性结合HIV-1的核衣壳（NC）蛋白。具有SEQ ID NO：3的氨基酸序列的肽肽C特异性结合HIV-1的核衣壳（NC）蛋白。具有SEQ ID NO：11的氨基酸序列的肽肽1特异性结合HIV-1的核衣壳（NC）蛋白。具有SEQ ID NO：12的氨基酸序列的肽肽2特异性结合HIV-1的核衣壳（NC）蛋白。具有SEQ ID NO：13的氨基酸序列的肽pep 3特异性结合HIV-1的核衣壳（NC）蛋白。

3. 发明公开

KR1020010090959A 사람의 면역결핍바이러스 억제제의 탐색을 위한 형질전환체 有权
标题翻译：用于检测人类免疫缺陷病毒的抑制剂的变体
公开(公告)号：KR1020010090959A
公开(公告)日：2001-10-22
申请号：KR1020000018489
申请日：2000-04-08
申请人： 유지창
发明人： 유지창 , 남혁준 , 김상헌
IPC分类号： C12N15/70
CPC分类号： C07K14/005 , C12N2740/16222 , C12Q1/18 , C12Q1/6897 , C12Q1/703
摘要： PURPOSE: Provided is a method for detecting an inhibitor of human immunodeficiency virus(HIV) using an E.coli strain JM109(KFCC-1159) cotransformed with vector pJC1 for the expression of HIV nucleocapside protein, and vector pNH1Psi(SL1234) including nucleotide sequence SL 1234(SEQ ID: NO.1) of HIV ψ and beta-galactosidase reporter gene(SEQ IN: NO.2). Therefore, it can be effectively used for the development of anti HIV inhibitor. CONSTITUTION: The detection method of HIV inhibitor comprises the steps of: culturing E.coli JM109(KFCC-11159) cotransformed with vector pJC1 and vector pNH1Psi(SL1234); treating the culture with a compound supposed to be an inhibitor of HIV; and measuring the expression level of beta-galactosidase in culture broth, decreased by the compound. The transformant characteristically decreases the expression of beta-galactosidase by linking nucleotide sequences of HIV protein and HIV ψ, and is thus used for the detection of HIV inhibitor.
摘要翻译：目的：提供使用与载体pJC1共转化的用于表达HIV核蛋白侧蛋白的大肠杆菌菌株JM109（KFCC-1159）和包含核苷酸序列的载体pNH1Psi（SL1234）来检测人免疫缺陷病毒（HIV）抑制剂的方法 HIVψ和β-半乳糖苷酶报告基因（SEQ IN：NO.2）的SL 1234（SEQ ID：NO.1）。因此，它可以有效地用于开发抗HIV抑制剂。构成：HIV抑制剂的检测方法包括以下步骤：培养用载体pJC1和载体pNH1Psi（SL1234）共转化的大肠杆菌JM109（KFCC-11159）; 用被认为是艾滋病毒抑制剂的化合物治疗文化; 并测定培养液中β-半乳糖苷酶的表达水平。转化体通过连接HIV蛋白和HIVψ的核苷酸序列特征降低β-半乳糖苷酶的表达，因此用于检测HIV抑制剂。

4. 发明授权

KR101158755B1 ＨＩＶＮＣ 단백질 발현용 벡터 및 이를 이용한 ＮＣ단백질의 생산방법 有权
标题翻译：用于表达HIV的NC蛋白的载体和使用其产生NC蛋白的方法
公开(公告)号：KR101158755B1
公开(公告)日：2012-06-22
申请号：KR1020060131392
申请日：2006-12-20
申请人： 유지창 , (주) 에빅스젠
发明人： 유지창
IPC分类号： C12N15/64 , C12N15/09
摘要： The present invention relates to a vector for expressing an NC protein of HIV and a method for producing an NC protein using the same. More particularly, the present invention relates to a vector for expressing an NC protein of HIV, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, and a method for producing an NC protein using the same. The vector for expressing an NC protein of HIV of the present invention, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, can express a wild type NC protein in animal cells, and has an effect of improving the expression efficiency, as compared to a known art.

5. 发明公开

KR1020080076614A ＨⅠＶＮＣ 단백질을 포함하는 ＡⅠＤＳ 예방 및 치료용조성물 有权
标题翻译：用于预防和治疗包含HIV阳性蛋白的艾滋病的组合物
公开(公告)号：KR1020080076614A
公开(公告)日：2008-08-20
申请号：KR1020070016786
申请日：2007-02-16
申请人： 유지창 , (주) 에빅스젠
发明人： 유지창
IPC分类号： A61K38/16 , A61P31/18
CPC分类号： A61K38/162 , C12N2740/16322 , G01N33/56988
摘要： A composition comprising human immunodeficiency virus nucleocapsid(HIV NC) protein is provided to inhibit proliferation of human immunodeficiency virus through the overexpression of HIV NC, so that the composition is useful for preventing and treating AIDS(acquired immune deficiency syndrome). A composition for preventing and treating AIDS comprises a polypeptide comprising HIV NC protein having the amino acid sequence of SEQ ID NO:1, which is selected from SEQ ID NO:2 to SEQ ID NO:7. A HIV NC gene has the nucleotide sequence of SEQ ID NO:8. An expression vector contains a polynucleotide comprising the HIV NC gene, having the nucleotide sequence selected from SEQ ID NO:8 to SEQ ID NO:14. A method for screening a material useful for preventing and treating AIDS comprises the steps of: (a) co-culturing a recombinant cell expressing the polypeptide containing HIV NC protein and a candidate material; and (b) measuring the increase of polypeptide level in the cell.
摘要翻译：提供包含人免疫缺陷病毒核衣壳（HIV NC）蛋白质的组合物以通过HIV NC的过表达抑制人类免疫缺陷病毒的增殖，使得该组合物可用于预防和治疗AIDS（获得性免疫缺陷综合征）。用于预防和治疗AIDS的组合物包含含有选自SEQ ID NO：2至SEQ ID NO：7的具有SEQ ID NO：1的氨基酸序列的HIV NC蛋白的多肽。 HIV NC基因具有SEQ ID NO：8的核苷酸序列。表达载体含有包含HIV NC基因的多核苷酸，其具有选自SEQ ID NO：8至SEQ ID NO：14的核苷酸序列。筛选用于预防和治疗AIDS的材料的方法包括以下步骤：（a）共培养表达含有HIV NC蛋白多肽和候选物质的重组细胞; 和（b）测量细胞中多肽水平的增加。

6. 发明授权

KR100599455B1 외부 유전자가 형질도입된 염색체를 포함하는 사람의 면역결핍바이러스 억제제의 탐색을 위한 형질전환체 有权
标题翻译：用于筛选与外源DNA整合的HIV携带染色体的转化体
公开(公告)号：KR100599455B1
公开(公告)日：2006-07-12
申请号：KR1020030060613
申请日：2003-08-30
申请人： 유지창
发明人： 유지창
IPC分类号： C12N15/70
摘要： 본 발명은 사람의 면역결핍바이러스의 si RNA 염기서열로 전사될 수 있는 염기서열과 lacZ 유전자를 순차적으로 포함하는 염색체 및 NC 단백질을 발현하는 발현벡터를 포함하는 형질전환체 및 그를 이용한 사람의 면역결핍바이러스 억제제의 탐색방법에 관한 것이다. 본 발명의 형질전환체는 보다 효과적으로 사람의 면역결핍바이러스 억제제를 탐색하는데 사용될 수 있으므로, 항 HIV 억제제의 개발에 널리 활용될 수 있을 것이다.
사람의 면역결핍바이러스, NC 단백질, psi 염기서열

7. 实用新型

KR2019950011633U 환편기용 실텐션 장치 无效
标题翻译：圆型针织机用线张力装置
公开(公告)号：KR2019950011633U
公开(公告)日：1995-05-16
申请号：KR2019930020301
申请日：1993-10-05
申请人： 유지창
发明人： 유지창
IPC分类号： D04B15/44
摘要： 본고안은실(1)이통과하는안내공(2)이형성된텐션구(3)와실 안내공(4)(5)이각각형성된돌출편(6)(7) 그리고타측에형 지지편(8)이부착되는공지의스위치본체(9)에있어서일측벽(10)에실 안내용 U자봉(11)이일체로형성된텐션봉(12)을나사(13)로체결고정하여텐션봉(12)의 U자봉(11)이항시상측으로탄발되도록구성하여서된 것을특징으로하는환편기용실 텐션장치를제공하여종래와같이별도의실 공급장치를사용하지않고텐션봉(12)을이용하여종래실 공급장치의역활을수행할수 있도록하고각기다른모양의구멍을형성할때마다실 공급장치의교체와같은번거로움및 여러종류의실 공급기를구비하여야하는경제적부담을해소할수 있도록하는데그 목적이있는것이다.

8. 发明授权

KR100987004B1 ＡＩＤＳ 예방 및 치료 물질 스크리닝용 재조합 포유동물세포 및 이를 이용한 스크리닝 방법 有权
标题翻译：用于筛选预防和治疗AIDS的物质的重组哺乳动物细胞和使用其的筛选方法
公开(公告)号：KR100987004B1
公开(公告)日：2010-10-11
申请号：KR1020080013918
申请日：2008-02-15
申请人： 유지창 , (주) 에빅스젠
发明人： 유지창
IPC分类号： C12N15/06 , C12N15/09 , C12N15/10 , C12N15/48
CPC分类号： G01N33/5008 , G01N2800/24
摘要： 본 발명은 AIDS 예방 및 치료 물질 스크리닝용 재조합 포유동물 세포 및 이를 이용한 스크리닝 방법에 관한 것으로, 보다 상세하게는, HIV NC 단백질을 발현하는 HIV NC 단백질 발현카세트(expression cassette)와 HIV Psi(Ψ) 서열 및 리포터 유전자를 발현하는 리포터 유전자 발현카세트(expression cassette)로 형질전환된 재조합 포유동물 세포 및 이를 이용한 스크리닝 방법에 관한 것이다. 본 발명의 재조합 포유동물 세포 및 스크리닝 방법은 진핵세포를 이용하여 HIV NC 단백질과 HIV Psi 서열의 상호작용을 저해하는 물질을 탐색할 수 있는 시스템을 제공하는 효과를 가진다. 따라서, 본 발명은 HIV의 포장에 관여하는 HIV NC 단백질 및 HIV Psi 서열의 상호작용을 저해하는 새로운 물질을 탐색할 수 있는 새로운 수단을 제공할 뿐만아니라 AIDS 예방 및 치료 효과를 가지는 물질을 탐색할 수 있는 새로운 스크리닝 방법을 제공하는 효과가 있다.
HIV NC 단백질, HIV Psi 서열, AIDS, 스크리닝

9. 发明公开

KR1020080057750A ＨＩＶＮＣ 단백질 발현용 벡터 및 이를 이용한 ＮＣ단백질의 생산방법 有权
标题翻译：用于表达HIV蛋白质的载体和使用其生产NC蛋白的方法
公开(公告)号：KR1020080057750A
公开(公告)日：2008-06-25
申请号：KR1020060131392
申请日：2006-12-20
申请人： 유지창 , (주) 에빅스젠
发明人： 유지창
IPC分类号： C12N15/64 , C12N15/09
摘要： An HIV NC(nucleocapsid) protein expression vector is provided to express a wild type NC protein in an animal cell and improve the expression efficiency of the NC protein compared with conventional techniques. An HIV NC protein expression vector is characterized in that at least one sequence selected from the group consisting of an SV40 19s mRNA intron sequence, a mutated SV40 16S mRNA intron sequence and a beta-globin intron sequence, an HIV NC gene, and an mRNA stability element selected from the group consisting of RRE(Rev response element), WPRE(woodchuck posttranscriptional regulatory element), beta-actin 3'-untranslated regions and RSV(Rous Sarcoma Virus) stability element are linked in sequence. A method for producing the HIV NC protein comprises a step of culturing a cell transformed by the vector, wherein the cell is COS-7 or 293T cell.
摘要翻译：提供HIV NC（核衣壳）蛋白表达载体，以在动物细胞中表达野生型NC蛋白，并提高NC蛋白与常规技术相比的表达效率。 HIV NC蛋白表达载体的特征在于，选自SV4019s mRNA内含子序列，突变SV40 16S mRNA内含子序列和β-珠蛋白内含子序列，HIV NC基因和mRNA中的至少一种序列选自RRE（Rev响应元件），WPRE（土拨鼠转录调控元件），β-肌动蛋白3'-非翻译区和RSV（Rous肉瘤病毒）稳定性元件的稳定性元件依次连接。制备HIV NC蛋白质的方法包括培养由载体转化的细胞的步骤，其中细胞是COS-7或293T细胞。

10. 发明公开

KR1020050022417A 외부 유전자가 형질도입된 염색체를 포함하는 사람의 면역결핍바이러스 억제제의 탐색을 위한 형질전환체 有权
标题翻译：改造高效筛选艾滋病毒抑制剂，将含有外源DNA和一种NC蛋白表达载体的染色体
公开(公告)号：KR1020050022417A
公开(公告)日：2005-03-08
申请号：KR1020030060613
申请日：2003-08-30
申请人： 유지창
发明人： 유지창
IPC分类号： C12N15/70
CPC分类号： C12N15/70 , C07K14/47 , C12Q1/025 , C12Q1/34
摘要： PURPOSE: A transformant for screening a HIV inhibitor harboring the chromosome integrated with foreign DNA is provided, thereby improving efficiency of HIV(human immunodeficiency virus) inhibitor screening method by using only one expression vector expressing a NC protein in comparison with the prior methods using two expression vectors. CONSTITUTION: The transformant for screening a HIV inhibitor harbors the chromosome containing a nucleotide sequence which can be transcribed into a psi RNA nucleotide sequence, and gene lacZ, sequentially, and an expression vector expressing NC protein. The method for screening the HIV inhibitor comprises the steps of: (i) culturing the transformant for screening the HIV inhibitor; (ii) treating the cultured transformant with a compound which is suspected as a HIV inhibitor; and (iii) measuring the expression level of beta-galactosidase from the compound treated transformant.
摘要翻译：目的：提供一种用于筛选携带与外来DNA整合的染色体的HIV抑制剂的转化体，从而通过仅使用一种表达NC蛋白的表达载体与现有方法相比较，使用两种方法，提高了HIV（人类免疫缺陷病毒）抑制剂筛选方法的效率表达载体。构成：用于筛选HIV抑制剂的转化体含有含有可以转录成psi RNA核苷酸序列和基因lacZ，以及表达NC蛋白的表达载体的核苷酸序列的染色体。筛选HIV抑制剂的方法包括以下步骤：（i）培养用于筛选HIV抑制剂的转化体; （ii）用疑似为HIV抑制剂的化合物处理培养的转化体; 和（iii）从化合物处理的转化体测量β-半乳糖苷酶的表达水平。

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