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    • 2. 发明授权
    • 미생물의 대량 생산을 위한 배양 배지
    • 文化中介大规模生产微生物
    • KR101298944B1
    • 2013-08-22
    • KR1020130027470
    • 2013-03-14
    • 한국해양과학기술원
    • 강도형아부아판허수진오철홍김지형전선미김주연예보람권영경장지이
    • C12N1/12C12R1/89
    • PURPOSE: A culture medium is provided to produce a large amount of microorganisms. CONSTITUTION: A culture medium contains 200-240 mg/L of NaNO_3, 200-240 mg/L of KNO_3, 130-200 mg/L of P_2O_5, 50-100 mg/L of KH_2PO_4, 20-45 mg/L of MgSO_4·7H_2O, 25-45 mg/L of Mg(NO_3)_2·6H_2O, 50-100 mg/L of Ca(NO_3)_2, 25-45 mg/L of K_2SO_4, 20-45 mg/L of Na_2-EDTA·2H_2O, 0.5-1.5 mg/L of FeCl_3·6H_2O, 0.01-1.0 mg/L of Co(NO_3)·6H_2O, 0.01-1.0 mg/L of MnSO_4·5H_2O, 0.01-1.0 mg/L of ZnSO_4·7H_2O, 0.001-0.1 mg/L of CuSO_4·5H_2O, 0.001-0.1 mg/L of (NH_4)_6Mo_7O_24·4H_2O, and 0.01-1.0 of mL/L HNO_3. The medium is used for culturing Haematococcus sp. KORDI03 (deposit number: KCTC12348BP) has a pH concentration of 9.0 or less.
    • 目的:提供培养基以产生大量的微生物。 构成:培养基含有200-240mg / L的NaNO_3,200-240mg / L的KNO_3,130-200mg / L的P_2O_5,50-100mg / L的KH_2PO_4,20-45mg / L的MgSO4 ·7H_2O,25-45mg / L的Mg(NO_3)_2·6H_2O,50-100mg / L的Ca(NO_3)_2,25-45mg / L的K_2SO_4,20-45mg / L的Na_2-EDTA ·2H_2O,0.5-1.5mg / L的FeCl_3·6H_2O,0.01〜1.0mg / L的Co(NO_3)·6H_2O,0.01〜1.0mg / L的MnSO4·5H_2O,0.01〜1.0mg / L的ZnSO_4·7H_2O, 0.001-0.1mg / L CuSO_4·5H_2O,0.001-0.1mg / L(NH_4)_6Mo_7O_24·4H_2O和0.01-1.0 mL / L HNO_3。 该培养基用于培养Haematococcus sp。 KORDI03(保藏号:KCTC12348BP)的pH值为9.0以下。
    • 3. 发明公开
    • 트리코데르마 속 J113 균주 유래 Ayt40 글리코시다아제 및 Ayt70 글리코시다아제, 및 이를 이용한 다당체의 가수분해방법
    • 来自木霉属J113菌株的Ayt40糖苷酶和Ayt70糖苷酶以及使用其的多糖的水解方法
    • KR1020170140493A
    • 2017-12-21
    • KR1020160072751
    • 2016-06-10
    • 한국해양과학기술원
    • 강도형이영득오철홍박건후
    • C12N9/24C12N9/26C12P7/06
    • C12N9/24C12N9/2414C12P7/06C12Y302/01001Y02E50/16
    • 본발명은트리코데르마속 J113 균주유래 Ayt40 글리코시다아제및 Ayt70 글리코시다아제, 및이를이용한다당체의가수분해방법에관한것으로, 더욱상세하게는트리코데르마속()의 J113 균주로부터유래되고, 녹말및 글리코겐의글라코시딕결합의무작위가수분해능을가지는알파-아밀라아제인 Ayt40 글리코시다아제와녹말의비환원성말단(non-reducing end)으로부터글루코스생성능을가지는글루코아밀라아제인 Ayt70 글리코시다아제를이용한다당체(polysaccharide)의가수분해방법에관한것이다. 본발명에따른트리코데르마속() J113 균주유래 Ayt40 글리코시다아제및 Ayt70 글리코시다아제를포함하는효소용액, 및이를이용한다당체의가수분해방법은시아노박테리아(cyanobacteria), 미세조류(microalgae) 또는식물세포유래바이오매스(biomass)에포함된다당체의당화효율(saccharification efficiency)을증가시킬수 있으므로바이오에탄올생산에유용하게이용할수 있다.
    • 使用衍生Ayt40糖苷酶和糖苷酶Ayt70本发明特里科DER马J113应变,并涉及多糖的水解方法是相同的,并且更具体地,从特里科DER马科动物(),淀粉和糖原J113菌株衍生 的格利登柯西迪克组合具有的一个尾数分辨率随机α - Ayt70的在非还原末端的Ayt40糖苷酶和淀粉酶(非还原端)葡糖淀粉酶糖苷酶与具有从葡萄糖生产能力的激酶多糖(多糖) 和一种水解方法。 特里科DER马科动物()J113菌株衍生Ayt40糖苷酶和含有激酶Ayt70糖苷酶溶液中,并使用相同的多糖的水解方法是二氰基细菌(蓝藻),藻类(微藻)或植物根据本发明的 由于它可以提高细胞衍生生物质中所含的多糖的糖化效率,因此可用于生产生物乙醇。
    • 6. 发明授权
    • 미세조류를 이용한 바이오매스의 생산 방법
    • 微生物生物量的生产方法
    • KR101298942B1
    • 2013-08-22
    • KR1020130027471
    • 2013-03-14
    • 한국해양과학기술원
    • 강도형아부아판허수진오철홍김지형전선미예보람권영경장지이김주연
    • C12N1/12C12P1/00
    • PURPOSE: A method for producing biomass using microalgae is provided to obtain biomass with high yield. CONSTITUTION: A method for producing biomass comprises the steps of: preparing a medium containing 200-240 mg/L of NaNO_3, 200-240 mg/L of KNO_3, 130-200 mg/L of P_2O_5, 50-100 mg/L of KH_2PO_4, 20-45 mg/L of MgSO_4·7H_2O, 25-45 mg/L of Mg(NO_3)_2·6H_2O, 50-100 mg/L of Ca(NO_3) _2, 25-45 mg/L of K_2SO_4, 20-45 mg/L of Na_2-EDTA·2H_2O, 0.5-1.5 mg/L of FeCl_3·6H_2O, 0.01-1.0 mg/L of Co(NO_3)·6H_2O, 0.01-1.0 mg/L of MnSO_4·5H_2O, 0.01-1.0 mg/L of ZnSO_4·7H_2O, 0.001-0.1 mg/L of CuSO_4·5H_2O, 0.001-0.1 mg/L of (NH_4) _6Mo_7O_24·4H_2O, and 0.01-1.0 mL/L of HNO_3; adjusting pH concentration to pH 9.0; and inoculating microalgae into the medium and culturing the microalgae.
    • 目的:提供使用微藻生产生物质的方法,以高产率获得生物质。 构成:生物质的生产方法包括以下步骤:制备含有200-240mg / L的NaNO_3,200-240mg / L的KNO_3,130-200mg / L的P_2O_5,50-100mg / L的 KH_2PO_4,20-45mg / L MgSO4·7H2O,25-45mg / L的Mg(NO_3)2·6H_2O,50-100mg / L的Ca(NO_3)2,25-45mg / L的K_2SO_4, 20-45mg / L的Na_2-EDTA·2H_2O,0.5-1.5mg / L的FeCl_3·6H_2O,0.01-1.0mg / L的Co(NO_3)·6H_2O,0.01-1.0mg / L的MnSO4·5H_2O,0.01 -1.0mg / L的ZnSO 4·7H 2 O,0.001-0.1mg / L的CuSO 4·5H 2 O,0.001-0.1mg / L的(NH 4)_6Mo_7O_24·4H_2O和0.01〜1.0mL / L的HNO 3; 将pH浓度调节至pH 9.0; 并将微藻接种到培养基中并培养微藻。