专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

KR1020130015123A 식물 이중-하이브리드 시스템을 이용한 단백질 상호작용 검출방법 및 검출키트 有权
标题翻译：使用植物双杂交系统检测蛋白质 - 蛋白质相互作用的方法和工具
公开(公告)号：KR1020130015123A
公开(公告)日：2013-02-13
申请号：KR1020110076960
申请日：2011-08-02
申请人： 한국생명공학연구원
发明人： 김차영 , 추효섭 , 우현숙 , 김민수 , 김중수 , 윤병대
IPC分类号： C12N15/82 , C12N15/29 , C12N15/62 , A01H5/00
摘要： PURPOSE: A method and a kit for detecting protein-protein interaction in a plant are provided to simply and accurately analyze anthocyanin pigment expression and to detect protein-protein interaction. CONSTITUTION: A method for detecting protein-protein interaction in a plant comprises a step of detecting interaction between prey proteins and bait proteins through a plant dual hybrid system using IbMYB1 transcription factor. The plant dual hybrid system comprises a DB vector and an AD vector. The DB vector contains: a promoter; a sequence encoding DNA-binding domain(DB-domain) of IbMYB1 transcription factor positioned in the downstream of the promoter; a linker sequence positioned in the downstream of the DB-domain; a multiple cloning site(MCS) positioned in the downstream of the linker; a terminator sequence positioned in the downstream of the MCS; and a sequence encoding the bait protein inside the MCS.
摘要翻译：目的：提供一种用于检测植物中蛋白质 - 蛋白质相互作用的方法和试剂盒，以简单准确地分析花青素色素表达并检测蛋白质 - 蛋白质的相互作用。构成：用于检测植物中蛋白质 - 蛋白质相互作用的方法包括通过使用IbMYB1转录因子的植物双重杂交系统检测猎物蛋白和诱饵蛋白之间的相互作用的步骤。植物双杂交系统包括DB载体和AD载体。 DB载体包含：启动子; 编码位于启动子下游的IbMYB1转录因子的DNA结合结构域（DB-domain）的序列; 位于DB结构域下游的接头序列; 位于接头下游的多克隆位点（MCS）; 位于MCS下游的终止符序列; 以及编码MCS内的诱饵蛋白的序列。

IPRDB

热门服务

关于我们

友情链接

联系方式