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2. 发明公开

KR1020060118836A 미생물 분류 검출 방법 失效
标题翻译：分类和检测微生物的方法
公开(公告)号：KR1020060118836A
公开(公告)日：2006-11-24
申请号：KR1020050041227
申请日：2005-05-17
申请人： 한국생명공학연구원
发明人： 박용하 , 배진우 , 이성근 , 남영도 , 박자령
IPC分类号： C12Q1/04
CPC分类号： Y02A50/451 , C12Q1/6834 , C12Q1/689
摘要： A method for classifying and detecting microorganisms is provided to be able to accurately classify and detect the microorganisms into subspecies by using a micro-array simply prepared by directly printing an amplified gene of pathogenic microorganisms without cloning step. The method comprises the steps of: (a) amplifying a gene of pathogenic microorganism such as salmonella through a suppression subtractive hybridization(SSH) technique; (b) printing the SSH product to prepare a micro-array; and (c) hybridizing the micro-array with a labeled sample and then detecting a signal. In the method, the SSH product obtained from the step(a) is not separated by cloning, but is directly printed on the micro-array.
摘要翻译：提供了分类和检测微生物的方法，以便能够通过使用简单地通过直接印刷扩增的病原微生物基因而不进行克隆步骤制备的微阵列来将微生物精确地分类并检测为亚种。该方法包括以下步骤：（a）通过抑制消减杂交（SSH）技术扩增病原微生物如沙门氏菌的基因; （b）打印SSH产品以准备微阵列; 和（c）将微阵列与标记样品杂交，然后检测信号。在该方法中，从步骤（a）获得的SSH产物不通过克隆分离，而是直接印刷在微阵列上。

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