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    • 3. 发明公开
    • 클로스트리디움 속 균주, 상기 균주를 이용한 부티르산 생산방법 및 상기 균주의 분리방법
    • CLOSTRIDIUM SP。 菌株,使用柠檬酸生产丁酸的方法。 菌株和用于分离菌丝体的方法。 应变
    • KR1020130077296A
    • 2013-07-09
    • KR1020110145926
    • 2011-12-29
    • 한국과학기술연구원
    • 엄영순이경민김연제상병인공경택
    • C12N1/20C12P7/52C12R1/145
    • PURPOSE: A butyric acid producing method using a strain from Clostridium is provided to effectively produce butyric acid from galactan, a marine algae biomass, having a main carbohydrate component by producing butyric acid form galactose. CONSTITUTION: A strain from Clostridium uses galactose to produce butyric acid. A butyric acid producing method from galactose comprises treating the strain from the Clostridium on galactose and culturing to convert galactose to butyric acid. A separating method of the strain from the Clostridium comprises a step of removing a gram-negative bacteria by heat treating an anaerobic sludge, a step of inoculating the heat treated anaerobic sludge to the culture medium containing galactose and culturing, a step of selecting colonies with butyric acid resistancy by smearing the culturing solution obtained from the culturing to the culture medium containing butyric acid or the salt, and a step of separating the most butyric acid producing strain by measuring butyric acid of the selected colonies.
    • 目的:提供使用来自梭菌的菌株的丁酸生产方法,以有效地从半乳聚糖(海藻藻类生物质)中产生具有主要碳水化合物组分的丁酸,通过产生丁酸形成半乳糖。 构成:来自梭菌的菌株使用半乳糖来生产丁酸。 来自半乳糖的丁酸生产方法包括在半乳糖上处理来自梭菌的菌株并培养以将半乳糖转化为丁酸。 来自梭菌的菌株的分离方法包括通过热处理厌氧污泥去除革兰氏阴性细菌的步骤,将热处理的厌氧污泥接种到含有半乳糖的培养基中并培养的步骤,选择具有 通过将从培养获得的培养溶液涂抹到含有丁酸或其盐的培养基中的丁酸抗性,以及通过测定所选择的菌落的丁酸来分离最多的丁酸生产菌株的步骤。
    • 10. 发明公开
    • 1,3-프로판다이올의 분리 방법
    • 纯化1,3-丙二醇的方法
    • KR1020110065708A
    • 2011-06-16
    • KR1020090122328
    • 2009-12-10
    • 한국과학기술연구원
    • 엄영순상병인김경덕
    • C07C29/94C07C31/20C12P7/04
    • PURPOSE: A method for separating 1,3-propanediol is provided to efficiently separate 1,3-propanediol in high concentration through an applicable simple method. CONSTITUTION: A method for separating 1,3-propanediol comprises the steps of: adding single or mixed solution to a microorganism culture liquid including 1,3-propanediol to separate a first solvent layer including ,3-propanediol; inducing layer separation by changing a solvent component ratio of the first solvent layer; and separating the 1,3-propanediol-containing layer of the layer-separated first solvent layers from a second solvent layer. The single solvent is one or more selected from the group consisting of methyl-ethyl-ketone, ethyl acetate, di-ethyl ether and methyl tert-butyl ether.
    • 目的:提供一种分离1,3-丙二醇的方法,通过适用的简单方法有效分离高浓度的1,3-丙二醇。 构成:分离1,3-丙二醇的方法包括以下步骤:向包含1,3-丙二醇的微生物培养液中加入单一或混合溶液以分离包括3-丙二醇的第一溶剂层; 通过改变第一溶剂层的溶剂组分比来诱导层分离; 并将层分离的第一溶剂层的1,3-丙二醇的层与第二溶剂层分离。 单一溶剂是选自甲基 - 乙基 - 酮,乙酸乙酯,二 - 乙基醚和甲基叔丁基醚中的一种或多种。