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1. 发明公开

KR1020040003505A 과수 바이러스 검정용 프라이머 및 이를 이용한 바이러스검출방법 失效
标题翻译：用于检测果树感染病毒的检测器和使用其的果树感染病毒的筛选方法
公开(公告)号：KR1020040003505A
公开(公告)日：2004-01-13
申请号：KR1020020038226
申请日：2002-07-03
申请人： 학교법인 경희학원
发明人： 박홍렬 , 김현란 , 최용문 , 윤재승 , 백광희
IPC分类号： C12Q1/68
摘要： PURPOSE: Primers for detecting fruit tree infecting virus and a screening method of fruit tree infecting virus using the same are provided, thereby easily detecting infection of ASGV(apple stem grooving capillovirus), ACLSV(apple chlorotic leafspot trichovirus), CTV(citrus tristeza virus) and CTLV(citrus tatter leaf virus). CONSTITUTION: Primers for detecting fruit tree infecting virus are selected from the group consisting of ASGV-Pf primer having the nucleotide sequence of SEQ ID NO: 1, ASGV-Pr primer having the nucleotide sequence of SEQ ID NO: 2, ACLSV-CPf primer having the nucleotide sequence of SEQ ID NO: 3, ACLSV-CPr primer having the nucleotide sequence of SEQ ID NO: 4, CTLV-CPf primer having the nucleotide sequence of SEQ ID NO: 5, CTLV-CPr primer having the nucleotide sequence of SEQ ID NO: 6, CTV-CPf primer having the nucleotide sequence of SEQ ID NO: 7, and CTV-CPr primer having the nucleotide sequence of SEQ ID NO: 8. A method for screening fruit tree infecting virus comprises the steps of: (a) amplifying RNA extracted from a sample as a template using RT-PCR using one primer selected from ASGV-Pf primer, ASGV-Pr primer, ACLSV-CPf primer, ACLSV-CPr primer, CTLV-CPf primer, CTLV-CPr primer, CTV-CPf primer, and CTV-CPr primer; and (b) analyzing the size of PCR products.
摘要翻译：目的：提供用于检测果树感染病毒的引物和使用其的果树感染病毒的筛选方法，从而容易地检测ASGV（苹果茎开槽毛壳病毒），ACLSV（苹果褪绿叶斑病毛癣病毒），CTV（柑橘三叶病毒））和CTLV（柑橘叶病毒）。构成：用于检测果树感染病毒的引物选自具有SEQ ID NO：1的核苷酸序列的ASGV-Pf引物，具有SEQ ID NO：2的核苷酸序列的ASGV-Pr引物，ACLSV-CPf引物具有SEQ ID NO：3的核苷酸序列，具有SEQ ID NO：4的核苷酸序列的ACLSV-CPr引物，具有SEQ ID NO：5的核苷酸序列的CTLV-CPf引物，具有SEQ ID NO：5的核苷酸序列的CTLV-CPr引物 SEQ ID NO：6，具有SEQ ID NO：7的核苷酸序列的CTV-CPf引物和具有SEQ ID NO：8的核苷酸序列的CTV-CPr引物。8.一种用于筛选果树感染病毒的方法，包括以下步骤：（a）使用选自ASGV-Pf引物，ASGV-Pr引物，ACLSV-CPf引物，ACLSV-CPr引物，CTLV-CPf引物，CTLV-CPr引物的一种引物，使用RT-PCR扩增从样品提取的RNA作为模板，CTV-CPf引物和CTV-CPr引物; 和（b）分析PCR产物的大小。

2. 发明授权

KR100442987B1 과수 바이러스 검정용 프라이머 및 이를 이용한 바이러스검출방법 失效
标题翻译： 과수바이러스검정용프라이머및이를이용한바이러스검출방과
公开(公告)号：KR100442987B1
公开(公告)日：2004-08-04
申请号：KR1020020038226
申请日：2002-07-03
申请人： 학교법인 경희학원
发明人： 박홍렬 , 김현란 , 최용문 , 윤재승 , 백광희
IPC分类号： C12Q1/68
摘要： PURPOSE: Primers for detecting fruit tree infecting virus and a screening method of fruit tree infecting virus using the same are provided, thereby easily detecting infection of ASGV(apple stem grooving capillovirus), ACLSV(apple chlorotic leafspot trichovirus), CTV(citrus tristeza virus) and CTLV(citrus tatter leaf virus). CONSTITUTION: Primers for detecting fruit tree infecting virus are selected from the group consisting of ASGV-Pf primer having the nucleotide sequence of SEQ ID NO: 1, ASGV-Pr primer having the nucleotide sequence of SEQ ID NO: 2, ACLSV-CPf primer having the nucleotide sequence of SEQ ID NO: 3, ACLSV-CPr primer having the nucleotide sequence of SEQ ID NO: 4, CTLV-CPf primer having the nucleotide sequence of SEQ ID NO: 5, CTLV-CPr primer having the nucleotide sequence of SEQ ID NO: 6, CTV-CPf primer having the nucleotide sequence of SEQ ID NO: 7, and CTV-CPr primer having the nucleotide sequence of SEQ ID NO: 8. A method for screening fruit tree infecting virus comprises the steps of: (a) amplifying RNA extracted from a sample as a template using RT-PCR using one primer selected from ASGV-Pf primer, ASGV-Pr primer, ACLSV-CPf primer, ACLSV-CPr primer, CTLV-CPf primer, CTLV-CPr primer, CTV-CPf primer, and CTV-CPr primer; and (b) analyzing the size of PCR products.
摘要翻译：本发明提供了用于检测果树感染病毒的引物和使用其的果树感染病毒的筛选方法，从而容易地检测ASGV（苹果茎切槽毛细管病毒），ACLSV（苹果褪绿叶斑点病毒），CTV（柑橘三镰孢病毒）和CTLV（柑橘破烂叶病毒）。构成：用于检测果树感染病毒的引物选自具有SEQ ID NO：1的核苷酸序列的ASGV-Pf引物，具有SEQ ID NO：2的核苷酸序列的ASGV-Pr引物，ACLSV-CPf引物具有序列号3的碱基序列的ACLSV-CPr引物，具有序列号5的碱基序列的CTLV-CPf引物，具有序列号5的碱基序列的CTLV-CPr引物， SEQ ID NO：6，具有SEQ ID NO：7的核苷酸序列的CTV-CPf引物和具有SEQ ID NO：8的核苷酸序列的CTV-CPr引物。一种筛选果树感染病毒的方法，包括以下步骤：（a）使用选自ASGV-Pf引物，ASGV-Pr引物，ACLSV-CPf引物，ACLSV-CPr引物，CTLV-CPf引物，CTLV-CPr引物的一种引物，使用RT-PCR扩增从样品提取的RNA作为模板，CTV-CPf引物和CTV-CPr引物; 和（b）分析PCR产物的大小。

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