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    • 2. 发明公开
    • 디펜신을 코딩하는 씨에이디이에프1 유전자 및 이를이용한 식물병저항성 탐색방법
    • CADEF1基因编码和使用它的植物病害的筛选方法
    • KR1020050031688A
    • 2005-04-06
    • KR1020030067925
    • 2003-09-30
    • 학교법인고려중앙학원
    • 황병국도현미이성철
    • C12N15/29
    • C07K14/415C12N15/8271C12Q1/6895C12Q2600/13
    • A CADEF1 gene encoding defensin and a screening method of plant disease resistance using the same gene are provided, which gene enables to remove processes of crop cultivation in the field, plant disease causing bacteria inoculation and verification of plant disease occurrence in the plant disease resistance screening method, so that the gene is useful for rapid screening method of plant disease tolerance. The CADEF1 gene encoding defensin, derived from Capsicum annuum L. cv. Hanbyul has the nucleotide sequence shown in figure 1. The protein defensin encoded by the CADEF1 gene has the amino acid sequence shown in figure 1. The screening method of plant disease resistance comprises the steps of; isolating RNA from a red pepper plant which is inoculated or treated by cells or chemicals; developing the RNA on electrophoresis and transferring the developed gel to a nitran membrane; and reacting the nitran membrane with a CADEF1 gene probe treated with 32P, wherein the cell is selected from pathogenic or nonpathogenic cells of Xanthomonas campestris pv. vesicatoria.
    • 提供编码防御素的CADEF1基因和使用相同基因的植物病害抗性筛选方法,该基因能够去除田间作物栽培的过程,引起细菌接种的植物病害和植物病害抗性筛选中植物病害发生的验证 方法,使得该基因对于植物病害耐受性的快速筛选方法是有用的。 编码防御素的CADEF1基因,来源于辣椒 汉族具有图1所示的核苷酸序列。由CADEF1基因编码的蛋白防御素具有图1所示的氨基酸序列。植物抗病性的筛选方法包括以下步骤: 从接种或细胞或化学物质的红辣椒植物中分离RNA; 在电泳上开发RNA并将开发的凝胶转移到硝酸膜; 并使硝基膜与用32P处理的CADEF1基因探针反应,其中所述细胞选自野油菜黄单胞菌(Pseudomonas campestris pv。)的致病性或非致病性细胞。 vesicatoria。
    • 4. 发明公开
    • Mach-Zehnder 간섭계를 이용한 전광 직렬-병렬데이터 형식 변환 장치
    • 使用MACH-ZEHNDER干涉仪的全光序列并行数据形成转换装置
    • KR1020020047691A
    • 2002-06-22
    • KR1020000076226
    • 2000-12-13
    • 학교법인고려중앙학원
    • 박진우이성철
    • H04B10/00
    • G02B6/2861H04B10/505H04J14/02
    • PURPOSE: An all-optical serial-parallel data formation converting device using a Mach Zehnder interferometer is provided to implement all-optically the serial data signal into the parallel data or the parallel data into the serial data, thereby constructing the device with small elements and enhancing the processing speed. CONSTITUTION: An optical fiber delay line(1) stands in line an input clock signal in a row. A wavelength multiplexer(2) multiplexes the delayed clock signal. An input port(3) of an interferometer(MZI) inputs the output signal of the wavelength multiplexer(2) to the interferometer(MZI). An optical coupler 1(4) divides the signal of the input port(3) of the interferometer(MZI) into the upper and lower branches. Serial data are inputted to a serial data input unit(5) as a control signal. In an optical coupler 3(13), the inputted serial data are connected to the lower branch of the interferometer(MZI). A semiconductor optical amplifier 2(10) performs a phase change of the input clock signal by the serial data. An optical amplifier 1(9) constructs the upper branch of the interferometer(MZI). An output port(11) of the interferometer(MZI) connects the output of the interferometer and the wavelength reverse-multiplexer. The wavelength reverse-multiplexer(12) multiplexes reversely and divides the outputted parallel data. An optical fiber delay line(1-1) delays the divided parallel data for outputting simultaneously them.
    • 目的:提供使用马赫策德尔干涉仪的全光并行数据转换装置,将串行数据信号全部实现为并行数据或并行数据转换为串行数据,从而构建具有小元件的器件, 提高处理速度。 构成:光纤延迟线(1)连续输入一个输入时钟信号。 波长多路复用器(2)复用延迟的时钟信号。 干涉仪(MZI)的输入端口(3)将波长多路复用器(2)的输出信号输入到干涉仪(MZI)。 光耦合器1(4)将干涉仪(MZI)的输入端口(3)的信号分成上分支和下分支。 串行数据作为控制信号输入到串行数据输入单元(5)。 在光耦合器3(13)中,输入的串行数据连接到干涉仪(MZI)的下分支。 半导体光放大器2(10)通过串行数据执行输入时钟信号的相位变化。 光放大器1(9)构成干涉仪的上分支(MZI)。 干涉仪(MZI)的输出端口(11)连接干涉仪的输出端和波长反向多路复用器。 波长反向多路复用器(12)反向复用并分割输出的并行数据。 光纤延迟线(1-1)延迟分割的并行数据以便同时输出。
    • 6. 发明公开
    • 고추 한별 품종 유래의 CASAR 82A 단백질을코딩하는 유전자 염기서열 및 마커로의 이용
    • SASAR82A基因的核苷酸序列。 L. CV。 汉诺威及其作为标记的用途
    • KR1020030086078A
    • 2003-11-07
    • KR1020020024444
    • 2002-05-03
    • 학교법인고려중앙학원
    • 황병국이성철
    • C12N15/29
    • C07K14/415C12N15/82C12Q1/6895C12Q2600/13
    • PURPOSE: A nucleotide sequence of SASAR82A gene from Capsicum annuum. L. cv. Hanbyul and the use thereof as a marker are provided. The SASAR82A gene is expressed in response to various biological and environmental stresses and has plant disease resistance. CONSTITUTION: SASAR82A gene isolated from Capsicum annuum. L. cv. Hanbyul has the nucleotide sequence of SEQ ID NO: 1. A diagnostic marker for biological and environmental stresses contains the SASAR82A gene of SEQ ID NO: 1. A recombinant vector is constructed by cloning the SASAR82A gene of SEQ ID NO: 1 to a vector pBluescript SK(-) phagemid. A method for determining the exposure of host plants to biological and environmental stresses comprises inserting the diagnostic marker containing the SASAR82A gene of SEQ ID NO: 1 to a host plant, and assaying mRNA transcribed from the host plant.
    • 目的:来自辣椒的SASAR82A基因的核苷酸序列。 L.cv。 提供汉字及其作为标记的用途。 SASAR82A基因是响应各种生物和环境胁迫而表达的,具有抗病性。 构成:从辣椒分离的SASAR82A基因。 L.cv。 汉族具有SEQ ID NO:1的核苷酸序列。生物和环境胁迫的诊断标记包含SEQ ID NO:1的SASAR82A基因。通过将SEQ ID NO:1的SASAR82A基因克隆到载体 pBluescript SK( - )噬菌粒。 用于确定宿主植物对生物和环境胁迫的暴露的方法包括将含有SEQ ID NO:1的SASAR82A基因的诊断标记物插入宿主植物,并测定从宿主植物转录的mRNA。
    • 7. 发明公开
    • SLALOM을 이용한 전광 직/병렬 데이터 형식 변환 장치
    • 电子系列/平行数据转换器
    • KR1020020047690A
    • 2002-06-22
    • KR1020000076225
    • 2000-12-13
    • 학교법인고려중앙학원
    • 박진우이성철
    • G02B6/293
    • PURPOSE: An electrooptic series/parallel data form converter using an SLALOM is provided to considerably reduce the number of required optical elements and perform a high speed operation in order to convert data form from an electrooptic series to a parallel and from an electrooptic parallel to a series. CONSTITUTION: An optical fiber delay line(1) delays an input parallel clock signal. A wavelength multiplexer(2) wavelength-multiplexes the delayed parallel clock signal. An input port(3) of a first photo coupler(4) inputs an output of the wavelength multiplexer(2) to a loop(10). A first photo coupler(4) inputs a signal of an input port(3) to a loop(10). Parallel data are inputted to a parallel data input port(5). A second photo coupler(8) is coupled with the loop(10). A semiconductor optical amplifier(9) generates a phase change in an input signal according to a control signal. The loop(10) includes an optical fiber. An output port(11) of the first coupler(4) connects an output of the loop(10) to the wavelength multiplexer(2). A wavelength demultiplexer(12) wave-demultiplexes and separates parallel data.
    • 目的:提供使用SLALOM的电光级数/并行数据形式转换器,以显着减少所需光学元件的数量并执行高速操作,以将数据形式从电光系列转换为并行,并从电光平行于 系列。 构成:光纤延迟线(1)延迟输入并行时钟信号。 波长多路复用器(2)对延迟的并行时钟信号进行波长多路复用。 第一光耦合器(4)的输入端口(3)将波长多路复用器(2)的输出端输入到环路(10)。 第一光耦合器(4)将输入端口(3)的信号输入到环路(10)。 并行数据被输入到并行数据输入端口(5)。 第二光耦合器(8)与环路(10)耦合。 半导体光放大器(9)根据控制信号产生输入信号的相位变化。 环路(10)包括光纤。 第一耦合器(4)的输出端口(11)将环路(10)的输出连接到波长多路复用器(2)。 波长解复用器(12)对并行数据进行波解复用和分离。
    • 8. 发明授权
    • 고추 한별 품종 유래의 CASAR 82A 단백질을코딩하는 유전자 염기서열 및 마커로의 이용
    • 고추한별품종유래의CASAR 82A단백질을코딩하는유전자염기서열및마커로의이용
    • KR100436750B1
    • 2004-06-22
    • KR1020020024444
    • 2002-05-03
    • 학교법인고려중앙학원
    • 황병국이성철
    • C12N15/29
    • PURPOSE: A nucleotide sequence of SASAR82A gene from Capsicum annuum. L. cv. Hanbyul and the use thereof as a marker are provided. The SASAR82A gene is expressed in response to various biological and environmental stresses and has plant disease resistance. CONSTITUTION: SASAR82A gene isolated from Capsicum annuum. L. cv. Hanbyul has the nucleotide sequence of SEQ ID NO: 1. A diagnostic marker for biological and environmental stresses contains the SASAR82A gene of SEQ ID NO: 1. A recombinant vector is constructed by cloning the SASAR82A gene of SEQ ID NO: 1 to a vector pBluescript SK(-) phagemid. A method for determining the exposure of host plants to biological and environmental stresses comprises inserting the diagnostic marker containing the SASAR82A gene of SEQ ID NO: 1 to a host plant, and assaying mRNA transcribed from the host plant.
    • 目的:来自Capsicum annuum的SASAR82A基因的核苷酸序列。 L. cv。 Hanbyul及其作为标记的用途。 SASAR82A基因是针对各种生物和环境胁迫而表达的,并具有植物病害抗性。 构成:从辣椒中分离的SASAR82A基因。 L. cv。 Hanbyul具有SEQ ID NO:1的核苷酸序列。用于生物和环境胁迫的诊断标记包含SEQ ID NO:1的SASAR82A基因。通过将SEQ ID NO:1的SASAR82A基因克隆到载体 pBluescript SK( - )噬菌粒。 用于确定宿主植物对生物和环境胁迫的暴露的方法包括将含有SEQ ID NO:1的SASAR82A基因的诊断标记插入宿主植物,并测定从宿主植物转录的mRNA。
    • 9. 发明授权
    • 고추 한별 품종의 씨오닌 유전자 및 이를 이용한 식물병 저항성 탐색방법
    • 고추한별품종의씨오닌유전자및이를이용한식물병저항성탐색방
    • KR100394467B1
    • 2003-08-09
    • KR1020000056518
    • 2000-09-26
    • 학교법인고려중앙학원
    • 황병국이성철김영진
    • C12N15/29
    • PURPOSE: Provided are thionine gene of Capsicum annuum L. cv. Hanbyul and a probing method of resistance for plant diseases, caused by Xanthomonas campestris subsp. vesicatoria, Collectotrichum coccodes, Colletotrichum gloeosporioides and the like. CONSTITUTION: The probing method of resistance for plant diseases comprises the steps of: inoculating Xanthomonas campestris subsp. vesicatoria into Capsicum annuum L. cv. Hanbyul to infect its leaves; separating mRNA from the infected leaves then manufacturing cDNA by using reverse transcriptase; performing PCR therewith by using primers to manufacture cDNA library; screening thionine gene therefrom then sequencing it; inoculating bacterial pathogen, fungal pathogen, ethephon, salicylic acid, beta-aminobutyric acid, BTH and the like into leaves of Capsicum annuum L. cv. Hanbyul; extracting RNA therefrom then followed by performing electrophoresis and transferring it to a nitrane membrane; and reacting it with 32P labeled thionine gene to detect its expression.
    • 用途:提供辣椒的硫堇基因。 Hanbyul和由野油菜黄单胞菌(Xanthomonas campestris subsp。)引起的对植物病害的抗性探索方法。 (vesicatoria),Collectotrichum coccodes,Colletotrichum gloeosporioides等。 组成:对植物病害的抗性探测方法包括以下步骤:接种野油菜黄单胞菌(Xanthomonas campestris) 发酵粉进入Capsicum annuum L. cv。 Hanbyul感染它的叶子; 从感染的叶中分离mRNA,然后用逆转录酶制备cDNA; 用引物制备cDNA文库进行PCR; 从中筛选硫堇基因,然后测序; 在辣椒叶片中接种细菌病原菌,真菌病原,乙烯利,水杨酸,β-氨基丁酸,BTH等。 Hanbyul; 从中提取RNA然后进行电泳并将其转移到硝基膜上; 并使其与32P标记的硫堇基因反应以检测其表达。
    • 10. 发明授权
    • SLALOM을 이용한 전광 직/병렬 데이터 형식 변환 장치
    • SLALOM可以在任何情况下使用/병렬데이터형식변환장치
    • KR100377456B1
    • 2003-03-26
    • KR1020000076225
    • 2000-12-13
    • 학교법인고려중앙학원
    • 박진우이성철
    • G02B6/293
    • PURPOSE: An electrooptic series/parallel data form converter using an SLALOM is provided to considerably reduce the number of required optical elements and perform a high speed operation in order to convert data form from an electrooptic series to a parallel and from an electrooptic parallel to a series. CONSTITUTION: An optical fiber delay line(1) delays an input parallel clock signal. A wavelength multiplexer(2) wavelength-multiplexes the delayed parallel clock signal. An input port(3) of a first photo coupler(4) inputs an output of the wavelength multiplexer(2) to a loop(10). A first photo coupler(4) inputs a signal of an input port(3) to a loop(10). Parallel data are inputted to a parallel data input port(5). A second photo coupler(8) is coupled with the loop(10). A semiconductor optical amplifier(9) generates a phase change in an input signal according to a control signal. The loop(10) includes an optical fiber. An output port(11) of the first coupler(4) connects an output of the loop(10) to the wavelength multiplexer(2). A wavelength demultiplexer(12) wave-demultiplexes and separates parallel data.
    • 目的:提供一种使用SLALOM的电光串/并行数据格式转换器,以显着减少所需光学元件的数量并执行高速操作,以便将数据形式从电光系列平行转换为平行并且从电光平行转换为平行 系列。 构成:光纤延迟线(1)延迟输入并行时钟信号。 波长复用器(2)对延迟的并行时钟信号进行波长复用。 第一光电耦合器(4)的输入端口(3)将波长复用器(2)的输出输入到环路(10)。 第一光电耦合器(4)将输入端口(3)的信号输入到环路(10)。 并行数据输入到并行数据输入端口(5)。 第二光电耦合器(8)与环路(10)耦合。 半导体光放大器(9)根据控制信号在输入信号中产生相位变化。 环(10)包括光纤。 第一耦合器(4)的输出端口(11)将环路(10)的输出端连接到波长复用器(2)。 波长解复用器(12)对并行数据进行波解复用和分离。