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1. 发明公开

KR1020130117951A 제대-유래의 간엽줄기세포의 생산 방법 无效
标题翻译：制备脐带神经干细胞的方法
公开(公告)号：KR1020130117951A
公开(公告)日：2013-10-29
申请号：KR1020120040731
申请日：2012-04-19
申请人： 주식회사 휴림바이오셀
发明人： 김지향 , 도병록 , 한수연 , 조정아 , 김철근 , 김종훈 , 신규철
IPC分类号： C12N5/0775 , C12N5/02 , C12Q1/02
摘要： PURPOSE: A method for producing umbilical cord-derived mesenchymal stem cells is provided to effectively isolate mesenchymal stem cells by basically removing pollution sources. CONSTITUTION: A method for producing umbilical cord-derived mesenchymal stem cells comprises the steps of: extracting umbilical cord and washing the umbilical cord with a buffer solution containing antibiotics and antibacterial agents; injecting collagenase to umbilical vein of the umbilical cord and performing incubation to collect an enzyme-treated liquid; collecting cells from the enzyme-treated liquid; and culturing the cells in a medium and collecting plastic-adherent cells. [Reference numerals] (AA) Before asepticism; (BB) After asepticism; (CC) Occurrence rate of pollution; (DD) Processing method A; (EE) Processing method B
摘要翻译：目的：提供一种生产脐带间充质干细胞的方法，通过基本消除污染源有效地分离间充质干细胞。构成：生产脐带间充质干细胞的方法包括以下步骤：用含有抗生素和抗菌剂的缓冲溶液提取脐带并洗涤脐带; 将胶原酶注射到脐带的脐静脉，并进行温育以收集酶处理的液体; 从酶处理液中收集细胞; 并在培养基中培养细胞并收集塑料粘附细胞。（附图标记）（AA）无菌之前; （BB）无菌; （CC）污染发生率; （DD）处理方法A; （EE）处理方法B

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