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1. 发明公开

KR1020020060009A 신규한 원유분해 균주 및 그 생산방법 有权
标题翻译：用于降低原油的新型微生物及其生产方法
公开(公告)号：KR1020020060009A
公开(公告)日：2002-07-16
申请号：KR1020010001207
申请日：2001-01-09
申请人： 주식회사 에코솔루션 , 오영숙 , 정욱진
发明人： 문혜준 , 정욱진 , 오영숙 , 주춘성 , 김윤관
IPC分类号： C12N1/20
摘要： PURPOSE: Provided are a novel microorganism, Pseudomonas sp.CU1, for the degradation of crude oil, gasoline,diesel oil, lamp oil and the like and a process for production thereof. Therefore, it is possible to bioremediate the sol contaminated with crude oil. CONSTITUTION: Pseudomonas sp.CU1(KCTC 18065P) is characteristically isolated by the steps of: inoculating the soil contaminated with crude oil into a minimal medium added with oil as a carbon source then culturing the medium at 20-30 deg.C; smearing the cultured medium on nutrient agar medium to isolate microorganisms for degradation of crude oil; and selecting a strain showing excellent growth and oil degradation.
摘要翻译：目的：提供一种用于原油，汽油，柴油，灯油等的降解的新型微生物假单胞菌属sp.CU1及其生产方法。因此，可以将被原油污染的溶胶生物中和。构成：通过以下步骤特征分离假单胞菌属sp.CU1（KCTC 18065P）：将被原油污染的土壤接种到加入油作为碳源的基本培养基中，然后在20-30℃培养培养基; 在营养琼脂培养基上涂抹培养基以分离用于原油降解的微生物; 并选择显示优异生长和油降解的菌株。

2. 发明授权

KR100421653B1 신규한 윤활유 분해 균주 및 그 생산방법 有权
标题翻译： 신규한윤활유유해해균주및그생산방법
公开(公告)号：KR100421653B1
公开(公告)日：2004-03-10
申请号：KR1020010001430
申请日：2001-01-10
申请人： 주식회사 에코솔루션 , 오영숙 , 정욱진
发明人： 주춘성 , 정욱진 , 오영숙 , 문혜준 , 김윤관
IPC分类号： C12N1/20
摘要： PURPOSE: Provided are a novel microorganism which degrades a lubricant and its production process. The microorganism is useful for the bioremediation of lubricant contaminated soil and underground water. CONSTITUTION: The novel microorganism, Bacillus sp. LU1 strain(KCTC 18064P) degrades a lubricant which is non-degradable hydrocarbon consisting of C18-15, at 10-30 deg.C, pH 5-9, preferably at 20-25 deg.C, pH 6-7. It is separated by the steps of: culturing oil contaminated soil in a minimal medium containing a lubricant; smearing the cultured medium on a nutrient agar medium; and selecting a microorganism having excellent lubricant degradability.
摘要翻译：目的：提供降解润滑剂及其生产过程的新型微生物。微生物可用于污染土壤和地下水的生物修复。组成：新型微生物，芽孢杆菌属。 LU1菌株（KCTC 18064P）在10-30℃，pH 5-9，优选20-25℃，pH 6-7下降解由C18-15组成的不可降解烃的润滑剂。它通过以下步骤分离：在含有润滑剂的基本培养基中培养受油污染的土壤; 将培养基涂抹在营养琼脂培养基上; 并选择具有优异的润滑剂降解性的微生物。

3. 发明公开

KR1020020036902A 유류분해 저온균주 및 그 제조방법 有权
标题翻译：用于柴油降解的焦糖微生物及其制备方法
公开(公告)号：KR1020020036902A
公开(公告)日：2002-05-17
申请号：KR1020000066990
申请日：2000-11-11
申请人： 주식회사 에코솔루션 , 오영숙 , 정욱진
发明人： 송훈 , 정욱진 , 오영숙 , 문혜준 , 주춘성 , 김윤관
IPC分类号： C12N1/20
摘要： PURPOSE: A psychrophile microorganism for the degradation of diesel and process for preparation thereof are provided, therefore psychrophile microorganism can be effectively used in purifying the oil contaminated soil in winter. CONSTITUTION: The psychrophile microorganism Pseudomonas sp. CX3B(KCTC 18054P), which is capable of decomposing oil including diesel below 12 deg.C., is isolated from the soil. Its preparation process comprises the steps of: collecting samples from the various oil-contaminated soils; inserting the samples into a phosphate buffer solution, agitating and sonicating them at 4 deg.C for 1 minute; cultivating the sample solution in a medium containing diesel and crude oil as a sole carbon source at 5 deg.C and 12 deg.C; spreading the cultivated medium on an agar medium to select oil-decomposing colonies through the color change by 2,6-DCPIP(Dichlorophenol-Indophenol) reduction; and screening a microorganism showing the most excellent oil-decomposability and viability at low temperature.
摘要翻译：目的：提供一种用于降解柴油的精神嗜性微生物及其制备方法，因此，冬季精油微生物可有效用于净化油污染土壤。构成：嗜酸菌微生物假单胞菌属能够将低于12摄氏度的柴油分解的CX3B（KCTC 18054P）与土壤分离。其制备方法包括以下步骤：收集各种油污土壤的样品; 将样品插入磷酸盐缓冲溶液中，在4℃下搅拌和超声处理1分钟; 在含有柴油和原油作为唯一碳源的介质中，在5℃和12℃下培养样品溶液; 将培养的培养基撒在琼脂培养基上，通过2,6-DCPIP（二氯苯酚 - 靛酚）还原的颜色变化来选择油分解菌落; 并在低温下筛选显示出最优异的油分解性和活力的微生物。

4. 发明授权

KR100421655B1 신규한 원유분해 균주 및 그 생산방법 有权
标题翻译： 신규한원유분해균주및그생산방법
公开(公告)号：KR100421655B1
公开(公告)日：2004-03-10
申请号：KR1020010001207
申请日：2001-01-09
申请人： 주식회사 에코솔루션 , 오영숙 , 정욱진
发明人： 문혜준 , 정욱진 , 오영숙 , 주춘성 , 김윤관
IPC分类号： C12N1/20
摘要： PURPOSE: Provided are a novel microorganism, Pseudomonas sp.CU1, for the degradation of crude oil, gasoline,diesel oil, lamp oil and the like and a process for production thereof. Therefore, it is possible to bioremediate the sol contaminated with crude oil. CONSTITUTION: Pseudomonas sp.CU1(KCTC 18065P) is characteristically isolated by the steps of: inoculating the soil contaminated with crude oil into a minimal medium added with oil as a carbon source then culturing the medium at 20-30 deg.C; smearing the cultured medium on nutrient agar medium to isolate microorganisms for degradation of crude oil; and selecting a strain showing excellent growth and oil degradation.
摘要翻译：目的：提供一种用于降解原油，汽油，柴油，灯油等的新型微生物Pseudomonas sp.CU1及其生产方法。因此，可以对受原油污染的溶胶进行生物修复。构成：通过以下步骤将假单胞菌属菌株CU1（KCTC 18065P）特征性地分离：将被原油污染的土壤接种到添加有油作为碳源的基本培养基中，然后在20-30℃下培养培养基; 将培养基涂抹在营养琼脂培养基上以分离微生物以降解原油; 并选择显示优异生长和油降解的菌株。

5. 发明授权

KR100421654B1 유류분해 저온균주 및 그 제조방법 有权
标题翻译： 유류분해저온균주및그제조방법
公开(公告)号：KR100421654B1
公开(公告)日：2004-03-10
申请号：KR1020000066990
申请日：2000-11-11
申请人： 주식회사 에코솔루션 , 오영숙 , 정욱진
发明人： 송훈 , 정욱진 , 오영숙 , 문혜준 , 주춘성 , 김윤관
IPC分类号： C12N1/20
摘要： PURPOSE: A psychrophile microorganism for the degradation of diesel and process for preparation thereof are provided, therefore psychrophile microorganism can be effectively used in purifying the oil contaminated soil in winter. CONSTITUTION: The psychrophile microorganism Pseudomonas sp. CX3B(KCTC 18054P), which is capable of decomposing oil including diesel below 12 deg.C., is isolated from the soil. Its preparation process comprises the steps of: collecting samples from the various oil-contaminated soils; inserting the samples into a phosphate buffer solution, agitating and sonicating them at 4 deg.C for 1 minute; cultivating the sample solution in a medium containing diesel and crude oil as a sole carbon source at 5 deg.C and 12 deg.C; spreading the cultivated medium on an agar medium to select oil-decomposing colonies through the color change by 2,6-DCPIP(Dichlorophenol-Indophenol) reduction; and screening a microorganism showing the most excellent oil-decomposability and viability at low temperature.
摘要翻译：目的：提供一种降解柴油的嗜冷微生物及其制备方法，因此嗜冷微生物在冬季可有效用于净化油污土壤。组成：嗜冷微生物假单胞菌属。 CX3B（KCTC 18054P）能够分解含12℃以下柴油的油类，可从土壤中分离出来。其制备过程包括以下步骤：从各种油污土壤中收集样品; 将样品插入磷酸盐缓冲液中，在4℃搅拌并超声处理1分钟; 在5℃和12℃下在含有柴油和原油作为唯一碳源的培养基中培养样品溶液; 通过2,6-DCPIP（二氯苯酚 - 靛酚）还原的颜色变化将培养基铺展在琼脂培养基上以选择油分解菌落; 并筛选在低温下显示最优异的油分解性和活力的微生物。

6. 发明公开

KR1020020060388A 신규한 윤활유 분해 균주 및 그 생산방법 有权
标题翻译：新型微生物降解润滑剂及其生产工艺
公开(公告)号：KR1020020060388A
公开(公告)日：2002-07-18
申请号：KR1020010001430
申请日：2001-01-10
申请人： 주식회사 에코솔루션 , 오영숙 , 정욱진
发明人： 주춘성 , 정욱진 , 오영숙 , 문혜준 , 김윤관
IPC分类号： C12N1/20
摘要： PURPOSE: Provided are a novel microorganism which degrades a lubricant and its production process. The microorganism is useful for the bioremediation of lubricant contaminated soil and underground water. CONSTITUTION: The novel microorganism, Bacillus sp. LU1 strain(KCTC 18064P) degrades a lubricant which is non-degradable hydrocarbon consisting of C18-15, at 10-30 deg.C, pH 5-9, preferably at 20-25 deg.C, pH 6-7. It is separated by the steps of: culturing oil contaminated soil in a minimal medium containing a lubricant; smearing the cultured medium on a nutrient agar medium; and selecting a microorganism having excellent lubricant degradability.
摘要翻译：目的：提供降解润滑剂及其生产工艺的新型微生物。该微生物可用于润滑剂污染土壤和地下水的生物修复。构成：新型微生物，芽孢杆菌属 LU1菌株（KCTC 18064P）降解润滑剂，其为不可降解烃，其由C18-15，10-30℃，pH 5-9，优选20-25℃，pH6-7组成。通过以下步骤分离：在含有润滑剂的基本培养基中培养油污染的土壤; 在营养琼脂培养基上涂抹培养基; 并选择具有优异的润滑剂降解性的微生物。

7. 发明公开

KR1020000061612A 유류분해 균주 스핀고모나스 속 Ｄ3Ｋ1 失效
标题翻译：菌株PSEUDOMONAS SP。 D3K1食品油及其制备方法
公开(公告)号：KR1020000061612A
公开(公告)日：2000-10-25
申请号：KR1019990010776
申请日：1999-03-29
申请人： 정욱진
发明人： 정욱진 , 문혜준
IPC分类号： C12N1/20
摘要： PURPOSE: Provided is a strain Pseudomonas sp. D3K1 (KCTC 8935P) isolated and purified from the soil contaminated by oils. Also, a method for preparing the strain. The strain is useful for recovering the soil and purifying the water because it is excellent in decomposing the oils and carbohydrates. CONSTITUTION: The strain Pseudomonas sp. D3K1 is isolated and purified by the steps of : (1) obtaining the soil contaminated by oils; (2) isolating the strain decomposing oils, wherein the sample is pre-treated by mixing sterilized PBS and the soil in a ratio of 2:1 followed by homogenizing, ultrasonic desorbing and the sample is used as an inoculum in the culture; (3) screening the strain growing rapidly using oils as a unique carbon source by 2, 6-DCPIP (2, 6-dichlorophenolindophenol) test; (4) identifying the screened strain using MIDI method.
摘要翻译：目的：提供假单胞菌属从被油污染的土壤中分离和纯化D3K1（KCTC 8935P）。另外，制备菌株的方法。该菌株对于回收土壤和净化水是有用的，因为它分解油和碳水化合物是极好的。构成：菌株Pseudomonas sp。 D3K1通过以下步骤分离和纯化：（1）获得被油污染的土壤; （2）分离菌株分解油，其中样品通过以2:1的比例混合灭菌的PBS和土壤进行预处理，然后均化，超声解吸，并将样品用作培养物中的接种物; （3）通过2,6-DCPIP（2,6-二氯苯酚靛酚）试验筛选使用油作为独特碳源快速生长的菌株; （4）使用MIDI方法识别筛选菌株。

8. 发明授权

KR100464107B1 유류분해 미생물제제 및 그 제조방법 有权
标题翻译： 유류분해미생물제제및그제조방법
公开(公告)号：KR100464107B1
公开(公告)日：2004-12-31
申请号：KR1020000073791
申请日：2000-12-06
申请人： 정욱진
发明人： 문혜준
IPC分类号： C12N1/20
摘要： PURPOSE: Microbial consortium for the degradation of diesel and process for preparation thereof are provided, thereby improving the degradation rate of diesel and ecological adaptation of the microbial consortia. CONSTITUTION: The microbial consortium comprises Pseudomonas Y2G1 (KCTC 18049P), Pseudomonas Y2K4 (KCTC 18050P) and Sphingomonas D3K1 (KCTC 8935P), which are isolated from the oil-contaminated soil. The method for producing the microbial consortia comprises the steps of: collecting the oil-contaminated soil samples; inserting each sample into a minimal medium and subjecting them to ultrasonication at 4 deg. C for 15 minutes at intervals of 1 minute; inoculating each supernatant into a minimal medium containing diesel and culturing them at 4 deg. C for 4 weeks; subculturing the samples; plating them on nutrient broth agar and culturing them to isolate colonies grown; inoculating the colonies into a minimal medium containing DCPIP and culturing them at 25 deg. C for 1 week; determining the color change of the cultivated medium; and selecting microorganisms showing excellent growth activity.
摘要翻译：目的：提供用于降解柴油的微生物联合体及其制备方法，从而提高柴油的降解率和微生物联盟的生态适应性。构成：微生物聚生体包含从油污染土壤分离的假单胞菌Y2G1（KCTC 18049P），假单胞菌Y2K4（KCTC 18050P）和鞘氨醇单胞菌D3K1（KCTC 8935P）。生产微生物聚生体的方法包括以下步骤：收集油污染的土壤样品; 将每个样品插入基本培养基中并在4℃下进行超声波处理。以1分钟的间隔15分钟; 将每种上清液接种到含有柴油的基本培养基中并在4℃下培养它们。 C 4周; 传代培养样品; 将它们铺在营养肉汤琼脂上并培养它们以分离生长的菌落; 将菌落接种到含有DCPIP的基本培养基中并在25℃下培养它们。 C 1周; 确定栽培培养基的颜色变化; 并选择显示优异生长活性的微生物。

9. 发明授权

KR100346640B1 유류분해 균주 스핀고모나스 속 Ｄ3Ｋ1 失效
标题翻译： 31降油细菌鞘氨醇单胞菌 D3K1
公开(公告)号：KR100346640B1
公开(公告)日：2002-08-01
申请号：KR1019990010776
申请日：1999-03-29
申请人： 정욱진
发明人： 정욱진 , 문혜준
IPC分类号： C12N1/20
摘要： 본발명은유류오염토양에서분리한균주들을농화배양 DCPIP시험을거쳐유류분해균주를일차선별한다음, 상기유류분해균주중에서유류를유일한탄소원으로하는조건에서빠른성장을보이는균주를선별하는것을특징으로하는유류분해균주제조방법및 상기제조방법에의하여제조되는신규한균주스핀고모나스 D3K1에관한것으로, 본발명신규한균주는유류및 난분해성탄화수소류를오염원으로하는오염물질의분해에사용되어안정적이며환경친화적인수질및 토양정화방법을제공하는현저한효과가있다.

10. 发明公开

KR1020020044733A 유류분해 미생물제제 및 그 제조방법 有权
标题翻译：柴油降解微生物污染物及其制备方法
公开(公告)号：KR1020020044733A
公开(公告)日：2002-06-19
申请号：KR1020000073791
申请日：2000-12-06
申请人： 정욱진
发明人： 문혜준
IPC分类号： C12N1/20
摘要： PURPOSE: Microbial consortium for the degradation of diesel and process for preparation thereof are provided, thereby improving the degradation rate of diesel and ecological adaptation of the microbial consortia. CONSTITUTION: The microbial consortium comprises Pseudomonas Y2G1 (KCTC 18049P), Pseudomonas Y2K4 (KCTC 18050P) and Sphingomonas D3K1 (KCTC 8935P), which are isolated from the oil-contaminated soil. The method for producing the microbial consortia comprises the steps of: collecting the oil-contaminated soil samples; inserting each sample into a minimal medium and subjecting them to ultrasonication at 4 deg. C for 15 minutes at intervals of 1 minute; inoculating each supernatant into a minimal medium containing diesel and culturing them at 4 deg. C for 4 weeks; subculturing the samples; plating them on nutrient broth agar and culturing them to isolate colonies grown; inoculating the colonies into a minimal medium containing DCPIP and culturing them at 25 deg. C for 1 week; determining the color change of the cultivated medium; and selecting microorganisms showing excellent growth activity.
摘要翻译：目的：提供柴油降解微生物联合体及其制备方法，提高柴油降解率和微生物菌群生态适应性。构成：微生物联盟包括从油污染土壤中分离的假单胞菌Y2G1（KCTC 18049P），假单胞菌Y2K4（KCTC 18050P）和鞘氨醇单胞菌D3K1（KCTC 8935P）。生产微生物菌群的方法包括以下步骤：收集油污土壤样品; 将每个样品插入基本培养基中，并在4℃下对其进行超声波处理。 C间隔1分钟15分钟; 将每个上清液接种到含有柴油的基本培养基中并在4℃下培养。 C 4周; 传代培养样品; 将其铺在营养肉汤琼脂上，培养它们以分离生长的菌落; 将菌落接种到含有DCPIP的基本培养基中并在25℃培养。 C一周; 确定栽培培养基的颜色变化; 并选择显示优异生长活性的微生物。

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