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    • 6. 发明公开
    • 개 파보바이러스 예방 및 치료를 위한 파보바이러스 고항원성 캡시드 단백질 및 이를 포함하는 사료용 조성물
    • 用于饲喂含有犬类PARVOVIRUS的高抗原CAPSID蛋白的组合物
    • KR1020110137743A
    • 2011-12-23
    • KR1020110057372
    • 2011-06-14
    • 전남대학교산학협력단
    • 김태중이재일
    • C12N15/35C12N15/81C07K14/015A23K1/18
    • PURPOSE: A high antigenic capsid protein for preventing and treating canine pavovirus and a feed composition containing the same are provided to induce immune response in an intestinal tract. CONSTITUTION: A canine pavovirus recombinant protein(VP2) has antigenic capsid site of sequence number 4. An expression vector contains a gene encoding the recombinant protein. The expression vector is pGAP Za. A method for preparing the recombinant protein comprises: a step of amplifying a gene encoding the antigenic capsid site of canine pavovirus using a primer of sequence numbers 1 and 2; a step of inserting the gene into the expression vector pGAP Za to prepare a recombinant expression vector; a step of transforming the recombinant expression vector into host cells to prepare a transformant; and a step of culturing the cells in a medium containing Zeocin and collecting the cultured product. The host cells are yeast(Pichia pastoris). A composition for preventing or treating canine pavovirus infection contains the recombinant protein as an active ingredient.
    • 目的:提供用于预防和治疗犬类禽流感病毒的高抗原性衣壳蛋白和含有该病毒的饲料组合物以诱导肠道中的免疫应答。 构成:犬病毒重组蛋白(VP2)具有序列号4的抗原性衣壳位点。表达载体含有编码重组蛋白的基因。 表达载体是pGAP Za。 一种制备重组蛋白质的方法,包括:使用序列号1和2的引物扩增编码犬类禽流感病毒抗原性衣壳位点的基因的步骤; 将基因插入表达载体pGAP Za以制备重组表达载体的步骤; 将重组表达载体转化到宿主细胞中以制备转化体的步骤; 以及在含有Zeocin的培养基中培养细胞并收集培养产物的步骤。 宿主细胞是酵母(巴斯德毕赤酵母)。 用于预防或治疗犬病毒感染的组合物含有重组蛋白作为活性成分。