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1. 发明公开

KR1020090054666A 부유밀도구배 원심분리를 이용하여 말초혈액단핵구로부터 단세포를 분리하는 방법 有权
标题翻译：通过浮选密度梯度离心从外周血单核细胞中分离单核细胞的分离方法及使用分离方法的细胞的制造方法
公开(公告)号：KR1020090054666A
公开(公告)日：2009-06-01
申请号：KR1020070121459
申请日：2007-11-27
申请人： 주식회사 바이넥스
发明人： 손철훈 , 박유수 , 이경규 , 김주인 , 배재호 , 강치덕 , 김선희
IPC分类号： C12M1/10 , C12M1/00
CPC分类号： C12M33/10 , C12M41/30
摘要： A method for isolating monocyte from peripheral blood mononuclear cell (PBMC) with floating density gradient centrifugation is provided to raise the purity and yield of monocyte by regulating density and osmotic pressure of histopaque or ficoll solution. A method for isolating monocyte from peripheral blood mononuclear cell (PBMC) with floating density gradient centrifugation is centrifuging PBMC using histopaque or ficoll solution. A density and osmotic pressure of histopaque or ficoll solution are 1.071-1.073g/ml and 330-340mOsm, respectively. A method for applying the density and osmotic pressure of histopaque or ficoll with determined figure is mixing PBS and NaCl solution in histopaque or ficoll solution. A dendritic cell is obtained by culturing the isolated monocyte.
摘要翻译：提供用浮动密度梯度离心法从外周血单核细胞（PBMC）分离单核细胞的方法，通过调节histopaque或ficoll溶液的密度和渗透压来提高单核细胞的纯度和产量。用浮动密度梯度离心法从外周血单核细胞（PBMC）分离单核细胞的方法是使用histopaque或ficoll溶液离心PBMC。 histopaque或ficoll溶液的密度和渗透压分别为1.071-1.073g / ml和330-340mOsm。在确定的图中应用histopaque或ficoll的密度和渗透压的方法是将PBS和NaCl溶液混合在histopaque或ficoll溶液中。通过培养分离的单核细胞获得树突状细胞。

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