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    • 4. 发明公开
    • 스트렙토마이세스 속 유래의 신규한 지질분해효소 및 그의 생산 방법
    • 来自STREPTOMYCES SP的新鲜脂肪 CS326及其制备方法
    • KR1020130072533A
    • 2013-07-02
    • KR1020110140000
    • 2011-12-22
    • 조선대학교산학협력단
    • 유진철조승식최윤희박다정송재경성치남유하영
    • C12N9/20C12N1/20C07K1/16C07K16/40
    • PURPOSE: A lipase produced by Streptomyces sp. is provided to catalyze transesterification for producing biodiesel and to properly produce biodiesel. CONSTITUTION: A lipase for producing biodiesel is isolated from Streptomyces sp. (CS326) and is identified to have a single protein band through SDS-PAGE. The molecular weight of the lipase is 17 kDa. The optimal reaction temperature and pH of the lipase are 40 deg. C and pH 7.0. The lipase is a heat resistant enzyme. The lipid decomposition activity is increased to 100% by adding Triton X-100 and is suppressed to 5% by adding SDS. The lipid decomposition activity is completely suppressed in deoxycholic acid and is suppressed by 50% using EDTA and EGTA. The lipid decomposition activity is suppressed by Mg^2+, Fe^2+, Na^+, Mn^2+, Ca^2+, and Co^2+. The rate constant is Km=0.24mM and V_max=4.6mM/min/mg using Lineweaver-Burk plot.
    • 目的:由链霉菌属(Streptomyces sp。)生产的脂肪酶 被提供用于催化酯交换以生产生物柴油并适当地生产生物柴油。 构成:用于生产生物柴油的脂肪酶是从链霉菌属(Streptomyces sp。 (CS326),并通过SDS-PAGE鉴定为具有单个蛋白质条带。 脂肪酶的分子量为17 kDa。 脂肪酶的最佳反应温度和pH为40度。 C和pH 7.0。 脂肪酶是耐热酶。 通过加入Triton X-100将脂质分解活性提高至100%,并通过加入SDS将其抑制至5%。 脂肪分解活性在脱氧胆酸中完全抑制,并使用EDTA和EGTA抑制50%。 脂肪分解活性受到Mg ^ 2 +,Fe ^ 2 +,Na +,Mn ^ 2 +,Ca ^ 2 +和Co ^ 2 +的抑制。 使用Lineweaver-Burk图,速率常数为Km = 0.24mM,V_max = 4.6mM / min / mg。
    • 9. 发明公开
    • 고성능 액체 크로마토그래피에 의한 나르제니신의 정량분석방법
    • 诺卡霉素中NARGENIC的定量分析方法SP。 CS682文化高性能液相色谱
    • KR1020100048369A
    • 2010-05-11
    • KR1020080107490
    • 2008-10-31
    • 조선대학교산학협력단
    • 유진철송재경조승식
    • G01N30/02G01N30/00
    • PURPOSE: A quantitative analysis method of nargenicin is provided to rapidly and accurately analyze the amount of the nargenicin in a cell-free culture medium, and to apply the method for analyzing other bacterial culture materials. CONSTITUTION: A quantitative analysis method of nargenicin contained in Nocardia sp. CS682 cultures by a high performance liquid chromatography comprises the following steps: cultivating Nocardia species microorganisms and extracting the nargenicin from a culture broth using ethylacetate; detecting the nargenicin using a solvent a containing dipotassium phosphate and water, and a solvent B containing acetonitrile; forming the standard calibration curve of the nargenicin by plotting a concentration per peak region of the nargenicin; and quantitative-analyzing the nargenicin.
    • 目的:提供Nargenicin的定量分析方法,快速,准确地分析无细胞培养液中的nargenicin量,并应用其他细菌培养物质分析方法。 构成:Nocardia sp。中含有的nargenicin的定量分析方法。 通过高效液相色谱法进行CS682培养,包括以下步骤:培养诺卡氏菌属微生物,并使用乙酸乙酯从培养液中提取Nargenicin; 使用含有磷酸氢二钾和水的溶剂a和含乙腈的溶剂B检测Nargenicin; 通过绘制Nargenicin的每个峰区的浓度,形成Nargenicin的标准校准曲线; 并定量分析nargenicin。