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    • 3. 发明公开
    • 방어와 부시리의 감별 방법 및 그 키트
    • 用于分离SERIOLA QUINQUERADIATA和SERIOLA LALANDI及其套件的方法
    • KR1020120065147A
    • 2012-06-20
    • KR1020100126508
    • 2010-12-10
    • 재단법인 제주테크노파크
    • 정용환오대주오봉세정민민이경후김지현
    • C12Q1/68C12N15/12
    • C12Q1/683
    • PURPOSE: A method for distinguishing Seriola quinqueradiata and Seriola lalandi is provided to specifically distinguish the fishes using mitochondria genome DNA and restriction enzyme recognition site. CONSTITUTION: A method for distinguishing Seriola quinqueradiata and Seriola lalandi by RFLP analysis comprises: a step of preparing a biological sample from Seriola quinqueradiata and Seriola lalandi; a step of isolating mitochondria genome DNA from the biological sample; a step of amplifying the DNA sequence containing a sequence of sequence number 1 for identifying Accl restriction enzyme recognition site of Seriola quinqueradiata and a DNA sequence containing sequence numbers 2 and 3 for identifying Accl restriction enzyme recognition site of Seriola lalandi; and a step of treating Accl restriction enzyme to the amplified product.
    • 目的:提供一种区分枸杞子和Seriola lalandi的方法,用于特异性区分使用线粒体基因组DNA和限制酶识别位点的鱼类。 构成:通过RFLP分析区分枸杞子和Seriola lalandi的方法包括:从枸杞子和Seriola lalandi制备生物样品的步骤; 从生物样品中分离线粒体基因组DNA的步骤; 扩增含有序列号1序列的DNA序列的步骤,用于鉴定Seriola quinqueradiata的Accl限制酶识别位点和含有序列号2和3的DNA序列,用于鉴定Seriola lalandi的Accl限制酶识别位点; 以及将Accl限制酶处理到扩增产物的步骤。
    • 6. 发明公开
    • 옥돔과 옥두어의 감별 방법 및 그 키트
    • Branch us us for。。。。。。。。。。。。。。。。。。。。。。。。
    • KR1020120058704A
    • 2012-06-08
    • KR1020100119938
    • 2010-11-29
    • 재단법인 제주테크노파크
    • 정용환오대주오봉세정민민이경후김지현
    • C12Q1/68C12N15/12
    • C12Q1/683C12Q2600/156
    • PURPOSE: A method for distinguishing Branchiostegus japonicus and Branchiostegus albus is provided to determine Branchiostegus japonicus and Branchiostegus albus based on mitochondria genome DNA sequence. CONSTITUTION: A method for distinguishing Branchiostegus japonicus and Branchiostegus albus using RFLP analysis comprises: a step of preparing biological sample from Branchiostegus japonicus and Branchiostegus albus; a step of isolating genome DNA of mitochondria from the biological sample; a step of amplifying DNA sequence of sequence number 1 which is a recognition site of Xcml restriction enzyme of cytochrome B gene and amplifying a DNA sequence of sequence number 2; and a step of treating with Xcml restriction enzyme.
    • 目的:根据线粒体基因组DNA序列,提供一种鉴别芍药和Branch bus属的方法,以确定芍药和Branch。属。 构成:使用RFLP分析来区分Branch us和Branch A的方法包括:从Branch from和Branch of of of;;;;;;;;;;;;;;;; 从生物样品中分离线粒体的基因组DNA的步骤; 扩增作为细胞色素B基因的Xcml限制酶的识别位点的序列号1的DNA序列并扩增序列号2的DNA序列的步骤; 和用Xcml限制酶处理的步骤。