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    • 1. 发明公开
    • 중신 줄기세포주
    • MESONEPHRIC干细胞线
    • KR1020080047676A
    • 2008-05-30
    • KR1020060117430
    • 2006-11-27
    • 재단법인서울대학교산학협력재단
    • 이창규손혜영이유진김혜선김민구박치훈김정은
    • C12N5/07C12N5/071C12N5/00
    • A mesonephric stem cell line is provided to be differentiated into various cells, treat various diseases or disorders through cell therapy and be used for developing a transgenic animal used for xenotransplantation. A method for preparing a mesonephric stem cell line comprises the steps of: (a) obtaining a mesonephric stem cell from mesonephros of mammal; (b) culturing the mesonephric stem cell onto a feeder cell layer to form a colony; and (c) subculturing the colony to obtain the mesonephric stem cell line, wherein the mammal is human, pig, cattle, sheep, horse, rabbit, goat, mouse, hamster or rat and the feeder cell layer is a fibroblast layer. A mesonephric stem cell line is derived from the mesonephros of the mammal and is characterized in that it show a positive reaction regarding alkaline phosphatase and has the ability of being naturally differentiated into embryonic bodies. Further, the fibroblast is derived from ICR mouse, and a number of the fibroblast is 1.8 to 2.8x10^5 cell/ml.
    • 提供中肾干细胞系以分化成各种细胞,通过细胞治疗治疗各种疾病或病症,并用于开发用于异种移植的转基因动物。 制备中肾干细胞系的方法包括以下步骤:(a)从哺乳动物的中肾获得中肾干细胞; (b)将培养的中肾干细胞培养至饲养细胞层以形成菌落; 并且(c)传代培养菌落以获得中肾干细胞系,其中哺乳动物是人,猪,牛,绵羊,马,兔,山羊,小鼠,仓鼠或大鼠,饲养细胞层是成纤维细胞层。 中肾干细胞系衍生自哺乳动物的中肾,其特征在于它显示出对碱性磷酸酶的阳性反应,并具有天然分化成胚体的能力。 此外,成纤维细胞衍生自ICR小鼠,并且许多成纤维细胞是1.8至2.8x10 5细胞/ ml。