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1. 发明授权

KR100465347B1 효모에서 악티노바실러스 플루로뉴모니애의 재조합ＡｐｘⅡＡ 독소 단백질을 제조하는 방법 및사료첨가제로서의 이의 용도 有权
标题翻译：在ApxⅡA的基础上增加了一些功能，可以让用户更方便地使用它们。
公开(公告)号：KR100465347B1
公开(公告)日：2005-01-13
申请号：KR1020030028073
申请日：2003-05-01
申请人： 유한상 , 장용석 , 김대혁 , 양문식
发明人： 유한상 , 신성제 , 양문식 , 장용석 , 김대혁
IPC分类号： C12P21/00
摘要： PURPOSE: A preparation method of recombinant ApxIIA toxin protein of Actinobacillus pleuropneumoniae in yeast and the use thereof as an feedstuff additive are provided, thereby providing immunogenicity to a pig by orally feeding it with the recombinant ApxIIA toxin protein of Actinobacillus pleuropneumoniae. CONSTITUTION: The preparation method of recombinant ApxIIA toxin protein of Actinobacillus pleuropneumoniae in yeast comprises the steps of: constructing an expression vector YEpGPD-TER-apxIIA producing the recombinant ApxIIA toxin structure protein; transforming yeast such as Saccharomyces cerevisiae with the expression vector; and culturing the transformed yeast to express the recombinant ApxIIA toxin structure protein. The feedstuff additive comprises the recombinant ApxIIA toxin structure protein.
摘要翻译：目的：提供一种酵母中胸膜肺炎放线杆菌重组ApxIIA毒素蛋白的制备方法及其作为饲料添加剂的用途，由此通过口服喂养胸膜肺炎放线杆菌的重组ApxIIA毒素蛋白为猪提供免疫原性。本发明公开了一种酵母型胸膜肺炎放线杆菌重组ApxIIA毒素蛋白的制备方法，包括以下步骤：构建表达载体YEpGPD-TER-apxIIA，产生重组ApxIIA毒素结构蛋白; 用表达载体转化酵母例如酿酒酵母（Saccharomyces cerevisiae）; 培养转化的酵母以表达重组ApxIIA毒素结构蛋白。饲料添加剂包含重组ApxIIA毒素结构蛋白。

2. 发明公开

KR1020040094118A 효모에서 악티노바실러스 플루로뉴모니애의 재조합ＡｐｘⅡＡ 독소 단백질을 제조하는 방법 및사료첨가제로서의 이의 용도 有权
标题翻译：重组APXIIA对青蒿素的毒素蛋白质的制备方法及其作为饲料添加剂的用途，从而提供给PIG的免疫原性
公开(公告)号：KR1020040094118A
公开(公告)日：2004-11-09
申请号：KR1020030028073
申请日：2003-05-01
申请人： 유한상 , 장용석 , 김대혁 , 양문식
发明人： 유한상 , 신성제 , 양문식 , 장용석 , 김대혁
IPC分类号： C12P21/00
摘要： PURPOSE: A preparation method of recombinant ApxIIA toxin protein of Actinobacillus pleuropneumoniae in yeast and the use thereof as an feedstuff additive are provided, thereby providing immunogenicity to a pig by orally feeding it with the recombinant ApxIIA toxin protein of Actinobacillus pleuropneumoniae. CONSTITUTION: The preparation method of recombinant ApxIIA toxin protein of Actinobacillus pleuropneumoniae in yeast comprises the steps of: constructing an expression vector YEpGPD-TER-apxIIA producing the recombinant ApxIIA toxin structure protein; transforming yeast such as Saccharomyces cerevisiae with the expression vector; and culturing the transformed yeast to express the recombinant ApxIIA toxin structure protein. The feedstuff additive comprises the recombinant ApxIIA toxin structure protein.
摘要翻译：目的：提供酵母中胸膜肺炎放线杆菌的重组ApxIIA毒素蛋白的制备方法及其作为饲料添加剂的用途，通过口服给予其与胸膜肺炎放线杆菌的重组ApxIIA毒素蛋白质，向猪提供免疫原性。构成：酵母中胸膜肺炎放线杆菌的重组ApxIIA毒素蛋白的制备方法包括：构建产生重组ApxIIA毒素结构蛋白的表达载体YEpGPD-TER-apxIIA; 用表达载体转化酵母例如酿酒酵母（Saccharomyces cerevisiae）; 并培养转化的酵母以表达重组ApxIIA毒素结构蛋白。饲料添加剂包含重组ApxIIA毒素结构蛋白。

3. 发明授权

KR100468480B1 철을 축적하는 패리틴 단백질을 발현하는 효모 有权
标题翻译：以使其能够被使用
公开(公告)号：KR100468480B1
公开(公告)日：2005-01-27
申请号：KR1020000070806
申请日：2000-11-27
申请人： 김대혁 , 장용석 , 양문식
发明人： 김대혁 , 양문식 , 장용석
IPC分类号： C12N15/81
摘要： PURPOSE: A yeast expressing a ferritin protein for the bioavailability of an iron is provided, thereby preventing and treating an iron deficiency of human. CONSTITUTION: The yeast expressing ferritin proteins for the bioavailability of an iron is prepared by constructing a recombinant expression vector pYETFAG-1 containing Tadpole ferritin heavy chain gene(TFH, M12120), and transforming yeast with the recombinant expression vector pYETFAG-1, wherein the yeast is Saccharomyces cerevisiae(KCTC 0853BP), and the recombinant expression vector pYETFAG-1 contains a promoter ADH2-GPD(alcohol dehydrogenase II-glyceraldehyde-3-phosphate dehydrogenase) and a ura gene as a selection maker other than TFH gene.
摘要翻译：目的：提供表达铁生物利用度的铁蛋白蛋白质的酵母，从而预防和治疗人体缺铁症。构成：通过构建含有蝌蚪铁蛋白重链基因（TFH，M12120）的重组表达载体pYETFAG-1并用重组表达载体pYETFAG-1转化酵母来制备表达铁生物利用度的酵母表达铁蛋白蛋白质，酵母是酿酒酵母（KCTC 0853BP），重组表达载体pYETFAG-1含有启动子ADH2-GPD（醇脱氢酶II-甘油醛-3-磷酸脱氢酶）和作为选择标记的ura基因。

4. 发明公开

KR1020030082098A 돼지 유행성 설사병 바이러스 중화 에피토프 및 이의 이용 失效
标题翻译：中毒病毒性鼻炎病毒及其用途
公开(公告)号：KR1020030082098A
公开(公告)日：2003-10-22
申请号：KR1020020020644
申请日：2002-04-16
申请人： 장용석 , 김대혁 , 양문식
发明人： 장용석 , 양문식 , 김대혁
IPC分类号： C07K14/165
CPC分类号： C07K14/005 , A61K39/215 , A61K2039/552 , C07K16/10 , C12N2770/20011
摘要： PURPOSE: A neutralizing epitope for porcine epidemic diarrhea virus and the use thereof are provided, thereby effectively preventing and treating the infection of porcine epidemic diarrhea virus. CONSTITUTION: A neutralizing epitope for porcine epidemic diarrhea virus(PEDV) comprises a polypeptide of SEQ ID NO: 1, wherein X22 is Asp or Gly, X 23 is Ser or Leu, X24 is Ser or Gly, X25 is Ser or Gly, X51 is Thr or Arg, X58 is Asn or Ser, X114 is Leu or Phe, and X137 is Ile or Val. A gene encoding the neutralizing epitope for porcine epidemic diarrhea virus has the nucleotide sequence of SEQ ID NO: 2. An anti-serum to PEDV is produced by injecting the PEDV epitope to an animal and collecting the anti-serum to PEDV from the immunized animal. An antibody to PEDV is isolated from the anti-serum to PEDV. A vaccine composition comprises the PEDV epitope.
摘要翻译：目的：提供猪流行性腹泻病毒的中和表位及其用途，有效预防和治疗猪流行性腹泻病毒感染。构成：猪流行性腹泻病毒（PEDV）的中和表位包含SEQ ID NO：1的多肽，其中X22为Asp或Gly，X23为Ser或Leu，X24为Ser或Gly，X25为Ser或Gly，X51 是Thr或Arg，X58是Asn或Ser，X114是Leu或Phe，X137是Ile或Val。编码猪流行性腹泻病毒中和表位的基因具有SEQ ID NO：2的核苷酸序列。通过将PEDV表位注射到动物并从抗体动物中收集抗血清至PEDV产生PEDV的抗血清。将PEDV的抗体从抗血清分离至PEDV。疫苗组合物包含PEDV表位。

5. 发明授权

KR100492820B1 돼지 유행성 설사병 바이러스 중화 에피토프 및 이의 이용 失效
标题翻译：用于猪流感病毒性腹膜病毒的中和化合物并使用其
公开(公告)号：KR100492820B1
公开(公告)日：2005-05-31
申请号：KR1020020020644
申请日：2002-04-16
申请人： 장용석 , 김대혁 , 양문식
发明人： 장용석 , 양문식 , 김대혁
IPC分类号： C07K14/165
摘要： 본 발명은 돼지 유행성 설사병 바이러스 중화 에피토프 및 이의 이용에 관한것이다. 특히 본 발명은 돼지 유행성 설사병 바이러스에 대하여 목적항원으로 사용하여 효과적으로 상기 바이러스의 감염을 예방할 수 있는 서열번호 1의 폴리펩타이드를 포함하는 PEDV(porcine epidemic diarrhea virus) 에피토프를 제공한다.

6. 发明公开

KR1020020041062A 철을 축적하는 패리틴 단백질을 발현하는 효모 有权
标题翻译： YEAST表达一种用于铁的生物利用度的FERRITIN蛋白质
公开(公告)号：KR1020020041062A
公开(公告)日：2002-06-01
申请号：KR1020000070806
申请日：2000-11-27
申请人： 김대혁 , 장용석 , 양문식
发明人： 김대혁 , 양문식 , 장용석
IPC分类号： C12N15/81
CPC分类号： C12N15/81 , C12N1/16 , C12R1/865
摘要： PURPOSE: A yeast expressing a ferritin protein for the bioavailability of an iron is provided, thereby preventing and treating an iron deficiency of human. CONSTITUTION: The yeast expressing ferritin proteins for the bioavailability of an iron is prepared by constructing a recombinant expression vector pYETFAG-1 containing Tadpole ferritin heavy chain gene(TFH, M12120), and transforming yeast with the recombinant expression vector pYETFAG-1, wherein the yeast is Saccharomyces cerevisiae(KCTC 0853BP), and the recombinant expression vector pYETFAG-1 contains a promoter ADH2-GPD(alcohol dehydrogenase II-glyceraldehyde-3-phosphate dehydrogenase) and a ura gene as a selection maker other than TFH gene.
摘要翻译：目的：提供表达铁的生物利用度的铁蛋白的酵母，从而预防和治疗人的铁缺乏症。构成：通过构建含有ad ole铁蛋白重链基因（TFH，M12120）的重组表达载体pYETFAG-1和用重组表达载体pYETFAG-1转化酵母制备表达铁的生物利用度的铁蛋白的酵母，其中酵母是酿酒酵母（KCTC 0853BP），重组表达载体pYETFAG-1含有启动子ADH2-GPD（醇脱氢酶II-甘油醛-3-磷酸脱氢酶）和ura基因作为TFH基因以外的选择制剂。

7. 发明授权

KR100468482B1 효모 발현 시스템을 이용한 인터루킨-18 단백질의생산방법 失效
标题翻译： 효모발현시스템을이용소인터루킨-18단백질의생산방효
公开(公告)号：KR100468482B1
公开(公告)日：2005-01-27
申请号：KR1020020019806
申请日：2002-04-11
申请人： 양문식 , 장용석 , 김대혁
发明人： 양문식 , 김대혁 , 장용석
IPC分类号： C12N15/81
摘要： PURPOSE: A production method of interleukin-18 using a yeast expression system is provided, thereby mass producing extracellular cytokine which has higher biological activity than cytokine expressed from a microorganism. CONSTITUTION: A vector comprises (a) GPD(glyceraldehyde-3-phosphate dehydrogenase) promoter(GenBank accession number M13807) or AG promoter(GenBank accession number J01314); (b) extracellular secretion signal sequence; (c) cytokine gene; and (d) transcription termination signal sequence, wherein the extracellular secretion signal sequence is amylase 1A secretion signal sequence (Ramy1A, GenBank accession number X16509); the cytokine gene is a gene encoding interleukin-18 mature protein; the vector is pYEGIL18 or pYEAGIL18. A production method of interleukin-18 comprises the steps of: (a) inserting a heterogenous gene into a vector comprising GPD promoter or AG promoter, extracellular secretion signal sequence, cytokine gene and transcription termination signal sequence to prepare a recombinant vector; (b) transforming yeast with the recombinant vector; and (c) culturing the transformed yeast in a medium.
摘要翻译：目的：提供一种使用酵母表达系统的白细胞介素-18的生产方法，由此大量生产具有比从微生物表达的细胞因子更高生物活性的细胞外细胞因子。组成：载体包含（a）GPD（甘油醛-3-磷酸脱氢酶）启动子（GenBank登录号M13807）或AG启动子（GenBank登录号J01314）; （b）细胞外分泌信号序列; （c）细胞因子基因; 和（d）转录终止信号序列，其中细胞外分泌信号序列是淀粉酶1A分泌信号序列（Ramy1A，GenBank登录号X16509）; 细胞因子基因是编码白细胞介素-18成熟蛋白的基因; 载体是pYEGIL18或pYEAGIL18。白介素-18的生产方法包括以下步骤：（a）将异源基因插入包含GPD启动子或AG启动子，细胞外分泌信号序列，细胞因子基因和转录终止信号序列的载体中以制备重组载体; （b）用重组载体转化酵母; 和（c）在培养基中培养转化的酵母。

8. 发明公开

KR1020030080933A 효모 발현 시스템을 이용한 인터루킨-18 단백질의생산방법 失效
标题翻译：使用YEAST表达系统的白介素18的生产方法
公开(公告)号：KR1020030080933A
公开(公告)日：2003-10-17
申请号：KR1020020019806
申请日：2002-04-11
申请人： 양문식 , 장용석 , 김대혁
发明人： 양문식 , 김대혁 , 장용석
IPC分类号： C12N15/81
CPC分类号： C12N15/81 , C07K14/54
摘要： PURPOSE: A production method of interleukin-18 using a yeast expression system is provided, thereby mass producing extracellular cytokine which has higher biological activity than cytokine expressed from a microorganism. CONSTITUTION: A vector comprises (a) GPD(glyceraldehyde-3-phosphate dehydrogenase) promoter(GenBank accession number M13807) or AG promoter(GenBank accession number J01314); (b) extracellular secretion signal sequence; (c) cytokine gene; and (d) transcription termination signal sequence, wherein the extracellular secretion signal sequence is amylase 1A secretion signal sequence (Ramy1A, GenBank accession number X16509); the cytokine gene is a gene encoding interleukin-18 mature protein; the vector is pYEGIL18 or pYEAGIL18. A production method of interleukin-18 comprises the steps of: (a) inserting a heterogenous gene into a vector comprising GPD promoter or AG promoter, extracellular secretion signal sequence, cytokine gene and transcription termination signal sequence to prepare a recombinant vector; (b) transforming yeast with the recombinant vector; and (c) culturing the transformed yeast in a medium.
摘要翻译：目的：提供使用酵母表达系统的白细胞介素-18的生产方法，从而大量生产比微生物表达的细胞因子具有更高生物活性的细胞外细胞因子。构成：载体包含（a）GPD（甘油醛-3-磷酸脱氢酶）启动子（GenBank登录号M13807）或AG启动子（GenBank登录号J01314）; （b）细胞外分泌信号序列; （c）细胞因子基因; 和（d）转录终止信号序列，其中细胞外分泌信号序列是淀粉酶1A分泌信号序列（Ramy1A，GenBank登录号X16509）; 细胞因子基因是编码白细胞介素-18成熟蛋白的基因; 载体是pYEGIL18或pYEAGIL18。白细胞介素-18的制备方法包括以下步骤：（a）将异源基因插入包含GPD启动子或AG启动子的载体，细胞外分泌信号序列，细胞因子基因和转录终止信号序列以制备重组载体; （b）用重组载体转化酵母; 和（c）在培养基中培养转化的酵母。

9. 发明授权

KR100371264B1 무라인 그래뉼로사이트-매크로파지 콜로니 자극 인자를포함하는 재조합 벡터와 그것을 발현하는 재조합아스퍼질러스 나이거 有权
标题翻译： 무라인그래뉼로사이트 - 매크로파지콜로니자극인자를포함하는재조합벡터와그것을발현하는재조합아스퍼질러스나이
公开(公告)号：KR100371264B1
公开(公告)日：2003-02-05
申请号：KR1020000003480
申请日：2000-01-25
申请人： 김대혁 , 장용석 , 양문식
发明人： 김대혁 , 양문식 , 장용석
IPC分类号： C12N15/63
摘要： PURPOSE: Provided are a recombinant vector including murine granulocyte macrophage colony stimulating factor and recombinant Aspergillus niger expressing the factor. Transformed Aspergillus niger mass-produces murine granulocyte macrophage colony stimulating factor to overcome side effects caused by over dose of the factor and can be applied to clinic. CONSTITUTION: A recombinant vector, optionally being pANGM-CSF plasmid is composed of a promotor of glycelaldehyde 3-phosphoric acid dehydrogenase, a terminator of glycelaldehyde 3-phosphoric acid dehydrogenase and a murine granulocyte macrophage colony stimulating factor. A recombinant host cell can be selected from Aspergillus niger, Fusarium oxysporum, Cryphonectria parasitica, Aspergillus nidulans, and Aspergillus awamori. In particular, Aspergillus niger (KCTC 0661BP) is favorable. The optimum conditions for the expression of a recombinant protein are as follows: pH 5.0-6.0 and the temperature of 30-37 deg.C.
摘要翻译：目的：提供包含鼠粒细胞巨噬细胞集落刺激因子和表达该因子的重组黑曲霉的重组载体。转化黑曲霉质粒 - 产生鼠粒细胞巨噬细胞集落刺激因子以克服过量因子引起的副作用，并可用于临床。构成：任选地pANGM-CSF质粒的重组载体由甘油醛3-磷酸脱氢酶的启动子，甘油醛3-磷酸脱氢酶的终止子和鼠粒细胞巨噬细胞集落刺激因子组成。重组的宿主细胞可以选自黑曲霉，尖孢镰刀菌，寄生黑粉菌，构巢曲霉和泡盛曲霉。具体而言，黑曲霉（KCTC 0661BP）是有利的。表达重组蛋白质的最佳条件如下：pH 5.0-6.0，温度30-37℃。

10. 发明公开

KR1020010076071A 무라인 그래뉼로사이트-매크로파지 콜로니 자극 인자를포함하는 재조합 벡터와 그것을 발현하는 재조합아스퍼질러스 나이거 有权
标题翻译：重组载体，其中包括MURINE GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR AND RECOMBINANT ASPERGILLUS NIGER EXPRESSING THE SAME
公开(公告)号：KR1020010076071A
公开(公告)日：2001-08-11
申请号：KR1020000003480
申请日：2000-01-25
申请人： 김대혁 , 장용석 , 양문식
发明人： 김대혁 , 양문식 , 장용석
IPC分类号： C12N15/63
CPC分类号： C12N15/63 , C12N2511/00 , C12R1/685
摘要： PURPOSE: Provided are a recombinant vector including murine granulocyte macrophage colony stimulating factor and recombinant Aspergillus niger expressing the factor. Transformed Aspergillus niger mass-produces murine granulocyte macrophage colony stimulating factor to overcome side effects caused by over dose of the factor and can be applied to clinic. CONSTITUTION: A recombinant vector, optionally being pANGM-CSF plasmid is composed of a promotor of glycelaldehyde 3-phosphoric acid dehydrogenase, a terminator of glycelaldehyde 3-phosphoric acid dehydrogenase and a murine granulocyte macrophage colony stimulating factor. A recombinant host cell can be selected from Aspergillus niger, Fusarium oxysporum, Cryphonectria parasitica, Aspergillus nidulans, and Aspergillus awamori. In particular, Aspergillus niger (KCTC 0661BP) is favorable. The optimum conditions for the expression of a recombinant protein are as follows: pH 5.0-6.0 and the temperature of 30-37 deg.C.
摘要翻译：目的：提供重组载体，包括鼠粒细胞巨噬细胞集落刺激因子和表达该因子的重组黑曲霉。转化黑曲霉大量产生鼠粒细胞巨噬细胞集落刺激因子，以克服过量剂量引起的副作用，可应用于临床。构成：任选是pANGM-CSF质粒的重组载体由甘油醛3-磷酸脱氢酶的启动子，甘油醛3-磷酸脱氢酶的终止子和鼠粒细胞巨噬细胞集落刺激因子组成。重组宿主细胞可以选自黑曲霉，尖孢镰刀菌，寄生红细菌，构巢曲霉和泡盛曲霉。特别地，黑曲霉（KCTC 0661BP）是有利的。表达重组蛋白的最佳条件如下：pH 5.0-6.0，温度30-37℃。

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