会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 5. 发明公开
    • 폴리단백질, 프로단백질 및 단백질분해를 사용한 sORF작제물 포함 다중 유전자 발현 및 방법
    • 多种基因表达,包括SORF构建体和方法与聚丙烯酸,蛋白质和蛋白质和蛋白质
    • KR1020080031024A
    • 2008-04-07
    • KR1020087001691
    • 2006-07-21
    • 애브비 인코포레이티드
    • 카슨제럴드알지온웬디살펠트조헨게구지지에레기어딘에이쿤스윤
    • C12N15/64C07K16/18C07H21/04C12P21/08
    • C12N15/67C07K16/00C07K2319/50C07K2319/92C12N15/1055C12P21/02C12P21/06
    • Disclosed are useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell. Constructs comprising a single open reading frame (sORF) are described for protein expression including expression of multiple polypeptides. A primary translation product (a pro-protein or a polyprotein) contains polypeptides such as inteins or hedgehog family auto-processing domains, or variants thereof, inserted in frame between multiple protein subunits of interest. The primary product can also contain cleavage sequences such as other proteolytic cleavage or protease recognition sites, or signal peptides which contain recognition sequences for signal peptidases, separating at least two of the multiple protein subunits. The sequences of the inserted auto-processing polypeptides or cleavage sites can be manipulated to enhance the efficiency of expression of the separate multiple protein subunits. Also disclosed are independent aspects of conducting efficient expression, secretion, and/or multimeric assembly of proteins such as immunoglobulins. Where the polyprotein contains immunoglobulin heavy and light chain segments or fragments capable of antigen recognition, in an embodiment a selectable stoichiometric ratio is at least two copies of a light chain segment per heavy chain segment, with the result that the production of properly folded and assembled functional antibody is made. Modified signal peptides, including such from immunoglobulin light chains, are described.
    • 公开了使用在重组宿主细胞内处理的主要翻译产物来表达蛋白质的有用构建体和方法。 描述了包含单个开放阅读框(sORF)的构建体用于蛋白质表达,包括多个多肽的表达。 初级翻译产品(前蛋白质或多蛋白质)含有多肽,例如内含肽或刺猬蛋白家族自身处理结构域或其变体,其插入到感兴趣的多个蛋白质亚基之间。 初级产物还可以含有裂解序列,如其他蛋白水解切割或蛋白酶识别位点,或含有信号肽酶识别序列的信号肽,分离多个蛋白质亚基中的至少两个。 可以操纵插入的自动处理多肽或切割位点的序列,以提高分离的多个蛋白质亚基的表达效率。 还公开了进行蛋白质例如免疫球蛋白的有效表达,分泌和/或多聚体装配的独立方面。 当多蛋白含有免疫球蛋白重链和轻链片段或能够进行抗原识别的片段时,在一个实施方案中,可选择的化学计量比为每个重链段的轻链片段的至少两个拷贝,结果是适当地折叠和组装 制备功能性抗体。 描述了包含免疫球蛋白轻链的修饰的信号肽。