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    • 3. 发明公开
    • L―쓰레오닌의 제조방법
    • 一种生产L-苏氨酸的方法
    • KR1020050025391A
    • 2005-03-14
    • KR1020030062423
    • 2003-09-06
    • 씨제이 주식회사
    • 조재용이병춘김대철이진호박영훈
    • C12N1/21
    • C12P13/08
    • A method for producing L-threonine is provided, thereby improving production yield of L-threonine by inactivating a gene metJ which inhibits activity of a gene metL that regulates the biosynthesis pathway of threonine and methionine. A microorganism Escherichia coli FTR1221(KCCM-10392) having the chromosome wherein a gene metJ is inactivated, and producing L-threonine is provided, wherein the gene metJ is inactivated by insertion mutation; and the Escherichia coli FTR1221(KCCM-10392) has resistance against threonine analogues, lysine analogues, isoleucine analogues and methionine analogues. The method for producing L-threonine comprises the steps of: (a) culturing Escherichia coli FTR1221(KCCM-10392) in a medium; and (b) recovering L-threonine from the cultured medium.
    • 提供L-苏氨酸的制造方法,通过抑制抑制调节苏氨酸和甲硫氨酸的生物合成途径的基因metL的活性的基因metJ来提高L-苏氨酸的产率。 提供了具有基因metJ灭活的染色体并产生L-苏氨酸的微生物大肠杆菌FTR1221(KCCM-10392),其中基因metJ通过插入突变失活; 大肠杆菌FTR1221(KCCM-10392)具有抗苏氨酸类似物,赖氨酸类似物,异亮氨酸类似物和甲硫氨酸类似物的抗性。 生产L-苏氨酸的方法包括以下步骤:(a)在培养基中培养大肠杆菌FTR1221(KCCM-10392); 和(b)从培养基中回收L-苏氨酸。
    • 4. 发明授权
    • L-쓰레오닌의 제조방법
    • L-쓰레오닌의제조방법
    • KR100427480B1
    • 2004-04-27
    • KR1020010002373
    • 2001-01-16
    • 씨제이 주식회사
    • 김영철노갑수박재용김대철이진호옥승한
    • C12P13/08
    • PURPOSE: Provided is a process for producing L-threonine by using a microorganism of which chromosome DNA contains phosphoenolpyruvate carboxylase(ppc) gene and additionally at least one copy of phosphoenolpyruvate carboxylase(ppc) in its specific region, thereby dramatically increasing the production yield of L-threonine. CONSTITUTION: Phosphoenolpyruvate carboxylase(ppc) converses phosphoenolpyruvate into oxaloacetate which is a precursor for the biosynthesis of threonine. The production yield of L-threonine can be improved by increasing ppc gene expression. Wherein, the expression of ppc gene can be increased by increasing the copy number of ppc gene in chromosome DNA of a microorganism.
    • 目的:提供一种通过使用染色体DNA含有磷酸烯醇式丙酮酸羧化酶(ppc)基因的微生物和另外至少一个磷酸烯醇式丙酮酸羧化酶(ppc)拷贝在其特定区域生产L-苏氨酸的方法,由此显着提高 L-苏氨酸。 构成:磷酸烯醇式丙酮酸羧化酶(ppc)将磷酸烯醇丙酮酸转化为草酰乙酸,草酰乙酸是苏氨酸生物合成的前体。 通过增加ppc基因表达可以提高L-苏氨酸的产量。 其中,ppc基因的表达可以通过增加微生物染色体DNA中ppc基因的拷贝数来增加。
    • 5. 发明授权
    • 엘-쓰레오닌 생산 미생물 및 이를 이용한 엘-쓰레오닌 제조방법
    • 엘 - 쓰레오닌생산미생물및이를이용한엘 - 쓰레오닌제조방엘
    • KR100426807B1
    • 2004-04-13
    • KR1020010034559
    • 2001-06-19
    • 씨제이 주식회사
    • 김영철박재용김대철이진호옥승한
    • C12N1/21
    • PURPOSE: Provided are an L-threonine producing microorganism and a method for producing L-threonine using the same, thereby producing L-threonine, which can be used for animal feeds, food additives and medicines, in high yield. CONSTITUTION: The L-threonine producing microorganism, E.coli ABA/fus-pykF 16(KCCM-10269), is originated from E.coli pGMTN-PPC 12(KCCM-10236) which contains two of phosphoenol pyruvate carboxylase(ppc) coding genes and two of operons, and inhibits expression of pykA or pykF gene which encodes pyruvate carboxylase isozyme. L-threonine is manufactured by culturing E.coli ABA/fus-pykF 16(KCCM-10269) in a growth medium at 32 deg.C with stirring under aeration.
    • 目的:提供一种产生L-苏氨酸的微生物和使用该微生物生产L-苏氨酸的方法,从而以高产率生产可用于动物饲料,食品添加剂和药物的L-苏氨酸。 构成:产L-苏氨酸的微生物大肠杆菌ABA / fus-pykF16(KCCM-10269)起源于大肠杆菌pGMTN-PPC12(KCCM-10236),其含有两种磷酸烯醇丙酮酸羧化酶(ppc)编码 基因和两个操纵子,并且抑制编码丙酮酸羧化酶同功酶的pykA或pykF基因的表达。 L-苏氨酸是通过在通气下在搅拌下在32℃下在生长培养基中培养大肠杆菌ABA / fus-pykF16(KCCM-10269)而制备的。
    • 7. 发明授权
    • 에리스리톨의정제방법
    • 净化胡萝卜素的方法
    • KR100253423B1
    • 2000-04-15
    • KR1019970056790
    • 1997-10-28
    • 씨제이 주식회사
    • 정상철이동우전영중서승현김대철조영제
    • C12P19/00
    • PURPOSE: Provided is a method for purifying high-purity erythritol in broth including strains that produce erythritol in a high yield without ion exchange resin or activated carbon to eliminate impurities. CONSTITUTION: A method for purifying high-purity erythritol in a high yield is characterized by the following steps of: i) cultivating strains that produce erythritol in a medium including glucose to obtain broth containing the strains and erythritol; ii) heating the broth at 60-80 deg.C and cooling it to make the strains and minute impurities coagulated; and iii) filtering coagulated matters with filtering membranes of 0.2-0.8μm in its size and separating erythritol from the matters. And the strains include Aureobasidium sp., Moniliella sp., Candida zeylanoides, Candida lipolytica, and Trichosporonoides madida.
    • 目的:提供一种在肉汤中纯化高纯度赤藓糖醇的方法,包括不含离子交换树脂或活性炭以高产率生产赤藓糖醇以消除杂质的菌株。 构成:以高产率纯化高纯度赤藓糖醇的方法的特征在于以下步骤:i)培养在包含葡萄糖的培养基中产生赤藓糖醇的菌株以获得含有菌株和赤藓糖醇的肉汤; ii)在60-80℃加热肉汤并冷却,使菌株和微量杂质凝结; 和iii)用尺寸为0.2-0.8μm的滤膜过滤凝结物质,并从物质中分离赤藓糖醇。 并且菌株包括Aureobasidium sp。,Moniliella sp。,zeylanoides假丝酵母,解脂假丝酵母和Trichosporonoides madida。