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    • 3. 发明公开
    • Yap1 변이체 유전자를 포함하는 효모 균주의 HMF 저항성 증가용 조성물
    • 用于增加包含YAP1突变体的YEAST的羟甲基纤维素的耐受性的组合物
    • KR1020130012328A
    • 2013-02-04
    • KR1020110073453
    • 2011-07-25
    • 서울대학교산학협력단
    • 한지숙김대희
    • C12N1/19C12N15/81
    • PURPOSE: A composition containing a Yap1 mutant gene for enhancing HMF resistance of yeast is provided to enhance resistance to HMF which is a yeast growth inhibitor. CONSTITUTION: A composition for enhancing HMF(hydroxymethylfurfural) of yeast contains a Yap1 mutant gene encoding an amino acid sequence of sequence number 2 or an expression protein thereof. The Yap1 mutant gene has a base sequence of sequence number 1. The Yap1 mutant gene is inserted into an expression vector. A method for preparing HMF resistant yeast comprises a step of preparing a Yap1 mutant gene encoding an amino acid of sequence number 2; a step of preparing a recombinant vector containing the Yap1 mutant gene; and a step of transforming the recombinant vector into yeast.
    • 目的:提供含有用于增强酵母HMF抗性的Yap1突变基因的组合物,以增强对作为酵母生长抑制剂的HMF的抗性。 构成:用于增强酵母HMF(羟甲基糠醛)的组合物含有编码序列号2的氨基酸序列的Yap1突变基因或其表达蛋白。 Yap1突变基因具有序列号1的碱基序列。将Yap1突变基因插入表达载体中。 制备HMF抗性酵母的方法包括制备编码序列号2的氨基酸的Yap1突变基因的步骤; 制备含有Yap1突变基因的重组载体的步骤; 以及将重组载体转化为酵母的步骤。