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    • 5. 发明授权
    • 항동결 단백질을 이용하여 난자를 동결보존하는 방법
    • 使用抗体蛋白的OOCYTE CRYOPRESERVATION的方法
    • KR101097953B1
    • 2011-12-22
    • KR1020110019521
    • 2011-03-04
    • 서울대학교병원
    • 조준우서창석지병철
    • C12N5/075C12N5/02A01N1/00C07K14/00
    • A01N1/0221
    • PURPOSE: A cryopreservation method for an ovum is provided to minimize damage of the ovum and to enhance survival rate after freezing and thawing. CONSTITUTION: A cryopreservation method for cells comprises: a step of mixing 14 volume% of serum, 15 volume% of ethylene glycol(EG), and 15 volume% of propylene glycol; a step of adding 100-3000ng/ml of anti-freeze proteins to prepare a cryopreservation composition; and a step of suspending human ovum, human fertilized egg, and ovarian cells with the composition and freezing. The anti-freeze protein is anti-freeze protein type III. The serum is fetal bovine serum(FBS), bovine serum albumin(BSA), or synthetic substitute serum(SSS). The composition additionally contains sucrose.
    • 目的:提供卵的冷冻保存方法,以尽量减少卵子的损伤,并提高冻融后的存活率。 构成:细胞的冷冻保存方法包括:混合14体积%的血清,15体积%的乙二醇(EG)和15体积%的丙二醇的步骤; 加入100-3000ng / ml抗冻蛋白以制备冷冻保存组合物的步骤; 并且将组合物和组合物悬浮在人卵,人受精卵和卵巢细胞上。 抗冻蛋白是III型抗冻蛋白。 血清是胎牛血清(FBS),牛血清白蛋白(BSA)或合成替代血清(SSS)。 组合物另外含有蔗糖。