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2. 发明公开

KR1020090038464A 배아 줄기 세포를 이용한 형질전환 조류의 제조 방법 有权
标题翻译：使用胚胎干细胞生产转基因禽的方法
公开(公告)号：KR1020090038464A
公开(公告)日：2009-04-20
申请号：KR1020097003555
申请日：2007-08-09
申请人： 발네바
发明人： 바라르쉬,이사벨 , 바딸,룩 , 메딸리,마지 , 귀앙노,파비앙
IPC分类号： C12N5/07 , C12N5/02 , A01K67/027 , C12Q1/68
CPC分类号： C12N5/0606 , A01K2217/00 , A01K2227/30 , C12N2501/10 , C12N2501/105 , C12N2501/115 , C12N2501/125 , C12N2501/13 , C12N2501/23 , C12N2501/2306 , C12N2502/13 , A01K67/0271
摘要： The present invention concerns a method of culturing embryonic stem (ES) cells of avian, comprising the steps of: a) suspending ES cells originating from the blastoderm disk of fertilized un-incubated avian egg(s) in a basal culture medium supplemented with: insulin-like growth factor-1 (IGF-1) and ciliary neurotrophic factor (CNTF); and animal serum; and optionally, at least one growth factors selected in the group comprising interleukin 6 (Il-6), interleukin 6 receptor (Il-6R), stem cell factor (SCF), fibroblast growth factor (FGF), leukaemia inhibitory factor (LIF), interleukin 11 (Il-11), oncostatin and cardiotrophin; b) seeding the suspension of ES cells obtained in step a) on a layer of feeder cells and further culturing the ES cells for at least between 2 to 10 passages; c) optionally removing at least one growth factors selected SCF, FGF, Il-6, Il-6R, LIF, oncostatin and cardiotrophin and Il-11 from the culture medium; d) further culturing the ES cells in the medium of step c) on a layer of feeder cells.
摘要翻译：本发明涉及一种培养禽类胚胎干（ES）细胞的方法，包括以下步骤：a）将源自受精培养未孵化禽鸟卵胚细胞盘的ES细胞悬浮在补充以下物质的基础培养基中：胰岛素样生长因子-1（IGF-1）和睫状神经营养因子（CNTF）; 和动物血清; 并且任选地，选自包含白细胞介素6（II-6），白细胞介素6受体（II-6R），干细胞因子（SCF），成纤维细胞生长因子（FGF），白血病抑制因子（LIF））的组中的至少一种生长因子，，白介素11（Il-11），制瘤素和心肌营养素; b）将步骤a）中获得的ES细胞的悬浮液接种在饲养细胞层上，并进一步培养ES细胞至少2至10代; c）任选地从培养基中除去选自SCF，FGF，II-6，II-6R，LIF，制瘤素和心肌营养素和II-11的至少一种生长因子; d）在步骤c）的培养基中进一步培养ES细胞在饲养细胞层上。

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