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    • 3. 发明公开
    • 국내 육성밀 품종 판별용 SCAR 마커 및 이의 용도
    • 韩国育种小麦品种鉴定SCAR标记及其应用
    • KR1020150041716A
    • 2015-04-17
    • KR1020130120135
    • 2013-10-08
    • 대한민국(농촌진흥청장)
    • 강천식김경훈김학신김경호이춘기현종내박광근박기훈손재한신상현
    • C12N15/11C12Q1/68
    • C12Q1/6895C12N15/11C12Q1/6844C12Q2600/13
    • 본발명은서열번호 1 내지 12의올리고뉴클레오티드의프라이머세트의군으로부터선택되는하나이상의프라이머세트를포함하는 KWSM001 프라이머세트, KWSM002 프라이머세트, KWSM003 프라이머세트, KWSM004 프라이머세트, KWSM005 프라이머세트및 KWSM006 프라이머세트등 6개국내밀 품종판별용프라이머세트및 이를이용한국내밀 품종판별방법에관한것이다. 본발명에따른프라이머세트를이용하면올, 다홍, 청계, 조경, 탑동, 남해, 올그루, 고분, 조품, 연백, 신미찰 1호, 적중, 한백등 국내밀 13개품종을명확히판별할수 있으므로, 품종육성권자에대한보호를강화할수 있고, 품종혼입문제를해결하는데기여할수 있으며, 국내밀 품종의분자유전학적연구및 주요형태적특성분석에도이용될수 있다.
    • 本发明涉及用于区分家用小麦类型的六个引物组,包括KWSM001引物组,KWSM002引物组,KWSM003引物组,KWSM004引物组,KWSM005引物组和选择了至少一个引物组的KWSM006引物组 来自由序列号为1〜12的寡核苷酸引物组构成的引物组,以及使用该引物组鉴别家用小麦的方法。 根据本发明,引物组用于准确鉴别Ol,大红,中ye,约oung,Tap Tap Tap Tap,,,,,,,right right right right right right right right right right right right right right right right right right right right right right right right right right right right 的育种者,从而解决混合类型的问题,并用于研究遗传分子基因和分析主要性状。
    • 10. 发明公开
    • 콩 배아로부터 이소플라본 및 사포닌의 분리정제 방법
    • 来自大豆胚的ISOFLAVONE和SOYASAPONIN的分离和纯化方法
    • KR1020140026725A
    • 2014-03-06
    • KR1020120092147
    • 2012-08-23
    • 대한민국(농촌진흥청장)
    • 김선림권영업김욱한이재은김율호정건호김대욱이춘기김미정이유영김정태
    • C07K1/16C07K14/415
    • A23L33/105A23L11/05A23L11/07
    • The present invention relates to a method of separating and purifying isoflavone and soyasaponin from soybean embryos, and more specifically, to the method of separating and purifying isoflavone and soyasaponin from soybean embryos in which a processing time is reduced by separating extracts containing embryos separated from soybeans through preparative chromatography, thereby separating the isoflavone, which is a bioactive substance, and saponin at the same time; has excellent workability; is capable of purifying the isoflavone and saponin having high chemical purity; and can recycle soybean byproducts that are treated as waste, thereby reducing environmental pollution. [Reference numerals] (AA) Dry raw beans (40°C, 72 hours); (BB) Separate soybean embryos (sifter with 2.36, 2.0, 1.18 mm); (CC) Remove fat of hexane (room temperature, 48 hours); (DD) Extract methanol (90°C, reflux cooling extraction 6 hours, twice); (EE) Condense and remove protein and sugar (twice); (FF) Separate by a C_18 (125�, 55-105쨉m) column catridge; (GG) Divide isoflavone (ISF-1); (HH,LL) Freeze-dry; (II) Re-separate ISF-1 (gel permeation column); (JJ) Check characteristics of isoflavone (ISF-1-1. ISF-1-2); (KK) Divide four kinds of soyasaponin; (MM) Check a molecular weight of the soyasaponin (Q-TOF) (SP-1,SP-2,SP-3,SP-4)
    • 本发明涉及从大豆胚胎中分离和纯化异黄酮和大豆皂角苷的方法,更具体地说,涉及从大豆胚分离和纯化异黄酮和大豆皂角苷的方法,其中通过分离含有从大豆分离的胚胎的提取物来减少处理时间 通过制备色谱法,同时分离作为生物活性物质的异黄酮和皂苷; 具有优异的可加工性; 能够纯化具有高化学纯度的异黄酮和皂苷; 并可回收大量副产品,作为废物处理,从而减少环境污染。 (AA)干粗豆(40℃,72小时); (BB)分离大豆胚胎(筛分为2.36,2.0,1.18mm); (CC)除去己烷中的脂肪(室温48小时); (DD)萃取甲醇(90℃,回流冷却提取6小时,两次); (EE)冷凝并去除蛋白质和糖(两次); (FF)由C_18(125 ,55-105쨉m)色谱柱分开; (GG)除异黄酮(ISF-1); (HH,LL)冷冻干燥; (II)重新分离ISF-1(凝胶渗透柱); (JJ)检查异黄酮的特征(ISF-1-1。ISF-1-2); (KK)分四种大豆皂角苷; (MM)检查大豆皂苷(Q-TOF)(SP-1,SP-2,SP-3,SP-4)的分子量