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    • 4. 发明公开
    • 파종량 및 시비량 자동조절 방법 및 장치
    • 自动控制方法和设备,用于肥料施用和种子
    • KR1020120047166A
    • 2012-05-11
    • KR1020100108887
    • 2010-11-03
    • 대한민국(농촌진흥청장)
    • 이정태박석호이계준류종수김점순김주일이형복
    • A01C5/08A01C5/00A01C19/00
    • A01C7/06A01C5/08A01C19/02G01S19/01Y02P60/16
    • PURPOSE: A method and an apparatus for automatically controlling seeding and fertilizing amount are provided to improve seeding and fertilizing accuracy. CONSTITUTION: A method for automatically controlling seeding and fertilizing amount is as follows. The speed of a tractor is measured by using a GPS sensor(110). The speed of the tractor is obtained. The ascending and descending availability of a seed metering unit(120) equipped with a seed metering roller is determined. The speed of the tractor is measured by using the GPS sensor when the ascending and descending of the seed metering unit is impossible. A drive motor is driven according to the speed of the drive motor when the ascending and descending of the seed metering unit is possible.
    • 目的:提供一种自动控制播种和施肥量的方法和装置,以提高播种和施肥的准确性。 构成:自动控制播种和施肥量的方法如下。 通过使用GPS传感器(110)来测量拖拉机的速度。 获得拖拉机的速度。 确定配备有种子计量辊的种子计量单元(120)的上升和下降可用性。 当种子计量单元的上升和下降是不可能的时候,通过使用GPS传感器来测量拖拉机的速度。 当种子计量单元的上升和下降是可能的时候,驱动马达根据驱动马达的速度被驱动。
    • 7. 发明公开
    • 감자에서 발생하는 4종 바이러스의 동시 진단용 프라이머 세트 및 이를 이용한 진단 방법
    • 用于PVY,PLRV,PVX和PVS感染卵的多重检测引物和使用该方法诊断感染性疾病的方法
    • KR1020080056828A
    • 2008-06-24
    • KR1020060129854
    • 2006-12-19
    • 대한민국(농촌진흥청장)
    • 이영규김점순윤영남천정욱
    • C12N15/10C12N15/09C12N15/11
    • A primer for diagnosing potato virus is provided to diagnose specifically 4 kinds of potato viruses such as potato virus Y(PVY), potato leafroll virus(PLRV), potato virus X(PVX) and potato virus S(PVS) at the same time through one RT-PCR reaction, thereby saving cost and labor force of the diagnosis. A primer for diagnosing PVY, PLRV, PVX and PVS simultaneously consists of primers of SEQ ID : NOs. 1 to 8. A method for diagnosing potato virus infection comprises the steps of: (a) isolating nucleic acid from a potato infected or suspicious to be infected by at least one of the PVY, PLRV, PVX and PVS; and (b) subjecting the isolated nucleic acid to RT-PCR using the primers of SEQ ID : NOs. 1 to 8, where the RT-PCR is done by reverse-transcribing it at a temperature of 45 deg.C for 30 minutes, removing reverse transcriptase therefrom at a temperature of 94 deg.C for 4 minutes, repeating a reaction 35 times, the reaction consisting of denaturing for 30 seconds at a temperature of 94 deg.C, annealing for 30 seconds at a temperature of 50 deg.C and elongating for 60 seconds at a temperature of 72 deg.C, treating it at a temperature of 72 deg.C for 5 minutes and maintaining the temperature at 4 deg.C. Further, a concentration ratio of the SEQ ID : NOs. 1: the SEQ ID : NOs. 2: the SEQ ID : NOs. 3: the SEQ ID : NOs. 4 is10:10:1:3.
    • 提供用于诊断马铃薯病毒的引物,用于同时诊断马铃薯病毒Y(PVY),马铃薯卷叶病毒(PLRV),马铃薯病毒X(PVX)和马铃薯病毒S(PVS)等4种马铃薯病毒, 一次RT-PCR反应,从而节省诊断成本和劳动力。 用于诊断PVY,PLRV,PVX和PVS的引物同时由SEQ ID NO:的引物组成。 用于诊断马铃薯病毒感染的方法包括以下步骤:(a)从感染或可疑的马铃薯中分离核酸以被PVY,PLRV,PVX和PVS中的至少一种感染; 和(b)使用SEQ ID NO:的引物对分离的核酸进行RT-PCR。 1〜8,RT-PCR在45℃的温度下反转录30分钟,在94℃的温度下除去逆转录酶4分钟,重复反应35次, 该反应在94℃的温度下变性30秒,在50℃的温度退火30秒,在72℃的温度下延伸60秒,在72℃的温度下处理 5℃,保持温度在4℃。 此外,SEQ ID NO: 1:SEQ ID:NO。 2:SEQ ID:NO。 3:SEQ ID:NO。 4是10:10:1:3。