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1. 发明授权

KR101376157B1 양조간장 제조용 코지 및 종국의 글루타미나제 활성 측정방법 有权
标题翻译：大豆科学与科氏模具谷氨酰胺酶的测定方法
公开(公告)号：KR101376157B1
公开(公告)日：2014-03-27
申请号：KR1020130019791
申请日：2013-02-25
申请人： 대상 주식회사
发明人： 이주희 , 박탁현 , 이정미 , 이창경 , 진중현
IPC分类号： G01N33/02 , C12Q1/34
CPC分类号： C12Q1/34 , C12Y305/01002
摘要： The present invention relates to a method for measuring a glutaminase enzyme activity in measuring enzyme activities by extracting an enzyme liquid from a cozy culture, including the steps of: adding glutamine; measuring contents of a glutamic acid and the glutamine using a Biochemistry Analyzer system (YSI 2900); and comparing and analyzing enzyme activities by makng a rate of decomposing the glutamine into the glutamic acid into data. In the present invention, since the rate at which the cozy decomposes the glutamine into the glutamate in a manner of measuring a self-expression enzyme activity without decomposing a cozy cell inoculated with a corresponding microorganism under an actual solid culture condition of soy sauce is directly measured, time may be saved, accuracy may be high under a fermentation condition and costs may be saved. [Reference numerals] (AA) Indirect method; (BB) Direct method using YSI
摘要翻译：本发明涉及通过从舒适培养物提取酶液体来测定酶活性的谷氨酰胺酶活性的方法，包括以下步骤：添加谷氨酰胺; 使用生化分析系统（YSI 2900）测量谷氨酸和谷氨酰胺的含量; 并通过将谷氨酰胺分解成谷氨酸的速率进行数据比较和分析酶活性。在本发明中，由于在不分解在实际的酱油固体培养条件下用相应微生物接种的舒适细胞的方式，以测定自体表达酶活性的方式将谷氨酰胺舒适地分解成谷氨酸的速率直接测量时间可节省，发酵条件下的准确度可能较高，可节省成本。（附图标记）（AA）间接法; （BB）使用YSI的直接方法

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