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    • 3. 发明公开
    • 말초혈액단핵구 유래 자연 살해세포의 유도 및 증식 방법
    • 从外周血单核细胞衍生的自然杀伤细胞的增殖和扩增方法
    • KR1020130124916A
    • 2013-11-15
    • KR1020130051442
    • 2013-05-07
    • 고려대학교 산학협력단
    • 이경미임선아캐시안이
    • C12N5/0783C12N13/00A61K35/12
    • C12N5/0646A61K35/17A61K2039/5158C12N2501/2302C12N2502/1114C12N2502/30
    • The present invention relates to a method for inducing and proliferating natural killer cells derived from peripheral blood mononuclear cells, comprising co-culture of the peripheral blood mononuclear cells with radiation-irradiated Jurkat cells and radiation-irradiated Epstein-Barr virus transformed lymphocyte continuous line (EBV-LCL) cells as vegetative cells under the presence of cytokines. According to the present invention, the method is able to induce and proliferate a large amount of natural killer cells from a small amount of peripheral blood mononuclear cells without expensive equipment and expensive various cytokines, thereby remarkably enhancing the efficiency and efficacy of cancer prevention and treatment using the natural killer cells. [Reference numerals] (AA,BB) Number of NK cells (X10^5)
    • 本发明涉及一种诱导和增殖来源于外周血单核细胞的天然杀伤细胞的方法,其包括将外周血单核细胞与辐射照射的Jurkat细胞和放射照射的爱泼斯坦 - 巴尔病毒转染的淋巴细胞连续线( EBV-LCL)细胞作为细胞因子存在下的营养细胞。 根据本发明,该方法能够从少量的外周血单核细胞中诱导和增殖大量的天然杀伤细胞,而不需要昂贵的设备和昂贵的各种细胞因子,从而显着提高了癌症预防和治疗的效率和功效 使用自然杀伤细胞。 (附图标记)(AA,BB)NK细胞数(X10 ^ 5)