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    • 2. 发明公开
    • 설사병 백신활성 유전자를 발현하는 형질전환 벼 및 그의 생산방법
    • 用于表达具有二羧酸的疫苗活性的基因的转化米及其生产方法
    • KR1020110068431A
    • 2011-06-22
    • KR1020090125389
    • 2009-12-16
    • 경기도
    • 소호섭한영희이지영임재욱박경열김영호홍순성양문식김율호한범수
    • C12N15/82A01H1/00C12N15/29C07K19/00
    • C12N15/8258
    • PURPOSE: A transgenic rice plant which expresses a gene of diarrhea vaccine activity is provided to remarkably reduce the use of antibiotics. CONSTITUTION: A method for producing a transgenic rice plant which expresses A gene diarrhea vaccine activity comprises: a step of preparing a recombinant vector by inserting nontoxic B subunit gene of Vibrio cholerae of sequence number 1, nontoxtic B subunit gene of E.coli of sequence number 2, and bar gene into pMJ103 vector to prepare a recombinant vector; a step of inserting pMJ103 recombinant vector to Agrobacterium to prepare transformed strain; a step of inoculating transformed Agrobacterium and co-culturing; a step of selecting alive callus and performing secondary culture to obtained secondary cultured callus; a step of performing bright culture of the callus and rooting in MS0 medium; a step of harvesting rice transformant.
    • 目的:提供一种表达腹泻疫苗活性基因的转基因水稻,显着降低了抗生素的使用。 构成:生产表达A基因腹泻疫苗活性的转基因水稻植物的方法,包括:通过插入序列号1的无毒性B亚基基因,大肠杆菌的无毒性B亚基基因序列来制备重组载体的步骤 2号和bar基因转入pMJ103载体中制备重组载体; 将pMJ103重组载体插入农杆菌以制备转化菌株的步骤; 接种转化的土壤杆菌并共培养的步骤; 选择活的愈伤组织和进行二次培养以获得次级培养的愈伤组织的步骤; 在MS0培养基中培养愈伤组织和生根的步骤; 收获稻转化株的一步。