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2. 发明公开

KR1020090120705A Ｔｓｐ６６Ｅ 유전자가 녹아웃된 초파리 돌연변이체 失效
标题翻译： DROSOPHILA TSP66E基因敲除突变体
公开(公告)号：KR1020090120705A
公开(公告)日：2009-11-25
申请号：KR1020080046643
申请日：2008-05-20
申请人： 건국대학교 산학협력단
发明人： 조경상 , 한승엽 , 최가희 , 이민정
IPC分类号： C12N15/12 , C12N15/09 , C12N15/00
摘要： PURPOSE: A Drosophila Tsp66E gene knock-out mutant is provided to use as a model for searching human cancer metastasis suppression mechanism. CONSTITUTION: A Drosophila Tsp66E gene knock-out mutant is made from EY02264 mutant strain. A method for making the Drosophila Tsp66E gene knock-out mutant comprises: a step of mating a male Drosophila(EY02264/EY02264) of EY02264 mutant strain with a female Drosophila[P(ry+, ex2-3), Sb/TM6B, Tb] which has not mated; a step of selecting a male Drosophila[EY02264/P(ry+, ex2-3), Sb] having both EY02264 and Δ2-3 mutant strains among its offspring; a step of mating a male Drosophila with a female Drosophila which has Tb(Tubby) phenotype and TM6B balancer and has not mated; a step of selecting a male Drosophila(Tsp66Eex/TM6B, Tb) whose offspring has cut P transposable element; a step of mating the selected male Drosophila with female Drosophila which has TM6B balancer and Tb(Tubby) phenotype and has not mated; a step of mating offspring from the prior step with a female Drosophila which has not mated with a male Drosophila(Tsp66Eex/TM6B, Tb); and a step of confirming mutation.
摘要翻译：目的：提供果蝇Tsp66E基因敲除突变体作为搜索人类癌症转移抑制机制的模型。构成：果蝇Tsp66E基因敲除突变体由EY02264突变株制成。制作果蝇Tsp66E基因敲除突变体的方法包括：将EY02264突变株的雄性果蝇（EY02264 / EY02264）与雌性果蝇[P（ry +，ex2-3），Sb / TM6B，Tb] 没有配合; 在其后代中选择具有EY02264和Δ2-3突变株的雄性果蝇[EY02264 / P（ry +，ex2-3），Sb]的步骤; 将雄性果蝇与具有Tb（Tubby）表型和TM6B平衡器的雌性果蝇交配并没有交配的步骤; 选择其后代切割P转座元件的雄性果蝇（Tsp66Eex / TM6B，Tb）的步骤; 将选定的雄性果蝇与具有TM6B平衡剂和Tb（Tubby）表型并没有交配的雌性果蝇交配的步骤; 将雌雄果蝇与雄性果蝇（Tsp66Eex / TM6B，Tb）没有交配的先前步骤的后代的一个步骤; 和确认突变的步骤。

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