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1. 发明公开

KR1020080076492A 인간 ＩＬ-32 특이 항원결정기를 갖는 단일클론항체,특이항체분비 융합 세포주 및 항체들을 이용한 ＩＬ-32측정법 有权
标题翻译：使用单克隆抗体生产单克隆抗体和诊断系统的IL-32抗体，混合物的单克隆抗体
公开(公告)号：KR1020080076492A
公开(公告)日：2008-08-20
申请号：KR1020070016477
申请日：2007-02-16
申请人： 건국대학교 산학협력단
发明人： 김기홍 , 심정현 , 조민철 , 강정우 , 최희숙 , 서은희 , 송은영 , 이희구 , 박미영 , 윤도영
IPC分类号： C12N5/07 , C07K16/00 , C12Q1/68
CPC分类号： C07K16/244 , G01N2333/54 , Y10S435/975 , Y10S530/809 , C12N5/06
摘要： Monoclonal antibodies specific against IL-32 antigens inducing TNF-alpha(tumor necrosis factor-alpha) are provided to study refractory inflammation and cancer, and diagnose inflammation responses and cancer at an early stage, so that the monoclonal antibodies are useful in sandwich ELISA(Enzyme-Linked ImmunoSorbant Assay). The hybridoma cell lines KU32-07(KCLRF-BP-00149) and KU32-52(KCLRF-BP-00150) secrete monoclonal antibodies having immunoglobulin subtypes of IgG2b and IgG1, and recognizing 91st-131st amino acids of IL-32alpha and 71st-107th amino acids of IL-32beta, respectively. A method for measuring the presence and concentration of IL-3alpha comprises the steps of: coating polyclonal antibody against IL-32 to a plate; adding IL-32 into the plate to be connected to the polyclonal antibody; adding a monoclonal antibody into the plate to be connected to the IL-32alpha; adding a labeled antibody with immunoresponsibility into the monoclonal antibody; and activating the labeled material bound to the antibody.
摘要翻译：提供针对诱导TNF-α（肿瘤坏死因子-α）的IL-32抗原特异性的单克隆抗体以研究难治性炎症和癌症，并在早期诊断炎症反应和癌症，使得单克隆抗体可用于夹心ELISA（酶联免疫吸附测定）。杂交瘤细胞系KU32-07（KCLRF-BP-00149）和KU32-52（KCLRF-BP-00150）分泌具有IgG2b和IgG1免疫球蛋白亚型的单克隆抗体，并识别IL-32α和71st-IIIal的91st-131st氨基酸，分别为IL-32beta的第107位氨基酸。用于测量IL-3α的存在和浓度的方法包括以下步骤：将针对IL-32的多克隆抗体包被到平板上; 将IL-32加入到与多克隆抗体连接的板中; 向板中加入单克隆抗体连接到IL-32α; 向单克隆抗体中加入具有免疫反应性的标记抗体; 并激活与抗体结合的标记物质。

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