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    • 2. 发明公开
    • VEGF-A, VEGF-B 및 VEGF-C의 안티센스cDNA를 함유하는 재조합 아데노-연관바이러스(rAAV) 및 이를 함유하는 대장암, 방광암및/또는 폐암 특이적 유전자 치료제
    • 包含VEGF-A,VEGF-B和VEGF-C以及特定于大肠癌,乳腺癌和/或包含其中的肺癌的基因治疗药物的抗体亚型相关病毒的重组腺病毒病毒
    • KR1020070013802A
    • 2007-01-31
    • KR1020050068427
    • 2005-07-27
    • 박기랑김원재충북보건과학대학교 산학협력단안미영황석연조영화이희란
    • 박기랑안미영황석연조영화이희란김원재
    • C12N15/861C12N15/34
    • A recombinant adeno-associated virus comprising antisense cDNAs of VEGF-A(vascular endothelial growth factor-A), VEGF-B and VEGF-C and a gene therapeutic agent specific to large intestine cancer, bladder cancer and/or lung cancer comprising the same virus are provided to reduce proliferation of tumor cells by inhibiting expression of VEGF associated with angiogenesis which is necessary for proliferation and transition of tumor cells. The recombinant adeno-associated virus(rAAV) vector comprises an antisense cDNA of VEGF-A of SEQ ID NO:1, an antisense cDNA of VEGF-B of SEQ ID NO:4 and an antisense cDNA of VEGF-C of SEQ ID NO:7, wherein the rAAV vector is prepared by (a) transfecting an animal cell line with pAAV vector containing the antisense cDNA of VEGF-A of SEQ ID NO:1, the antisense cDNA of VEGF-B of SEQ ID NO:4 and the antisense cDNA of VEGF-C of SEQ ID NO:7, an AAV rep-cap plasmid DNA and an adenovirus helper plasmid, (b) culturing the transfected animal cell line, and (c) destroying the cultured animal cell line, and isolating and purifying the rAAV particle. The gene therapeutic agent specific to large intestine cancer, bladder cancer and/or lung cancer comprises the recombinant adeno-associated virus(rAAV) vector.
    • 包含VEGF-A(血管内皮生长因子-A),VEGF-B和VEGF-C的反义cDNA以及对大肠癌,膀胱癌和/或肺癌特异性的基因治疗剂的重组腺相关病毒 提供病毒以通过抑制肿瘤细胞增殖和转化所必需的血管生成相关VEGF的表达来减少肿瘤细胞的增殖。 重组腺相关病毒(rAAV)载体包含SEQ ID NO:1的VEGF-A的反义cDNA,SEQ ID NO:4的VEGF-B的反义cDNA和SEQ ID NO:4的VEGF-C的反义cDNA 7,其中rAAV载体通过以下步骤制备:(a)用含有SEQ ID NO:1的VEGF-A的反义cDNA,SEQ ID NO:4的VEGF-B的反义cDNA的pAAV载体转染动物细胞系,以及 SEQ ID NO:7的VEGF-C的反义cDNA,AAV rep-cap质粒DNA和腺病毒辅助质粒,(b)培养转染的动物细胞系,和(c)破坏培养的动物细胞系, 并纯化rAAV颗粒。 特异于大肠癌,膀胱癌和/或肺癌的基因治疗剂包括重组腺相关病毒(rAAV)载体。