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1. 发明公开

KR1020140045822A 혈소판 보존 조성물, 이를 포함하는 혈소판 보존 키트 및 이를 이용한 혈소판 보존 방법 有权
标题翻译：板保存组合物和用于保存板的工具箱以及使用该板保护板的方法
公开(公告)号：KR1020140045822A
公开(公告)日：2014-04-17
申请号：KR1020120112061
申请日：2012-10-09
申请人： 김홍승
发明人： 김홍승
IPC分类号： A01N1/00 , A61N1/02 , A61J1/12
CPC分类号： A01N1/02 , A61J1/12 , C12N5/0644
摘要： The present invention relates to a platelet preserving composition, a preserving kit comprising the same, and a method for preserving a platelet for a long time using the same and, more specifically, to a preserving composition for preserving a platelet for four weeks or more, the preserving kit comprising the same, and the method for preserving a platelet using the same.
摘要翻译：本发明涉及血小板保存组合物，含有该组合物的保存试剂盒，以及使用该组合物长时间保存血小板的方法，更具体地，涉及一种用于保存血小板保存4周以上的保存组合物，包含其的保存试剂盒以及使用其的保存血小板的方法。

2. 发明授权

KR101290061B1 말초 혈액으로부터 수지상 세포를 유도하기 위한 배양 조성물, 이를 포함하는 배양 키트 및 이를 이용하여 말초 혈액으로부터 수지상 세포를 유도하는 방법 有权
标题翻译：培养组合物和用于从外周血培养至细胞培养物的试剂盒，以及使用其从外周血中诱导感染细胞的方法
公开(公告)号：KR101290061B1
公开(公告)日：2013-07-26
申请号：KR1020120073804
申请日：2012-07-06
申请人： 김홍승
发明人： 김홍승
IPC分类号： C12N5/0784 , C12N5/02
CPC分类号： C12N5/0639 , C12N2500/38 , C12N2500/40 , C12N2501/01
摘要： PURPOSE: A culture composition, a culture kit for inducing a dendritic cell from leukocytes obtained from a peripheral blood, and a method for inducing the dendritic cell by using the same are provided to generate large numbers of dendritic cells and be used for the treatment of autoimmune disease and cell therapeutics. CONSTITUTION: A culture composition for inducing a dendritic cell from a peripheral blood comprises adenosine-triphosphate (ATP), divalent cation, chloride, vitamin B, insulin, granulocyte colony stimulating factors, erythropoietin, and solvent. The positive cell marker factors of the induced dendritic cells are CD14, CD11c, and CD68. The method to induce the dendritic cell from the peripheral blood comprises of the steps: obtaining Buffy coat and plasma through the centrifugation of the mix of blood and anticoagulant; injecting the Buffy coat and plasma to a sterilized airtight container which maintains equal pressure between the exterior and interior; and incubating the container with the Buffy coat and plasma in a cold incubator.
摘要翻译：目的：提供培养组合物，用于从由外周血获得的白细胞诱导树突细胞的培养试剂盒，以及通过使用该培养组合物诱导树突状细胞的方法，以产生大量的树突状细胞，并用于治疗自身免疫疾病和细胞治疗。构成：用于从外周血诱导树突状细胞的培养组合物包括三磷酸腺苷（ATP），二价阳离子，氯化物，维生素B，胰岛素，粒细胞集落刺激因子，促红细胞生成素和溶剂。诱导的树突状细胞的阳性细胞标记因子是CD14，CD11c和CD68。从外周血诱导树突状细胞的方法包括以下步骤：通过血液和抗凝剂混合物的离心获得巴菲涂层和血浆; 将Buffy涂层和等离子体注入到在外部和内部之间保持相等压力的灭菌气密容器中; 并在冷孵箱中将容器与Buffy外套和血浆一起孵育。

3. 发明授权

KR101595756B1 활성 혈소판 보존 조성물, 활성 혈소판 보존 방법 및 이를 이용하여 보존된 활성 혈소판 有权
标题翻译：活化板保存组合物，使用该活化板保存活化板和保存的活化板的方法
公开(公告)号：KR101595756B1
公开(公告)日：2016-02-19
申请号：KR1020150010167
申请日：2015-01-21
申请人： 김홍승
发明人： 김홍승
IPC分类号： A01N1/02 , C12N5/078
CPC分类号： A01N1/0226 , C12N5/0644 , A01N1/021
摘要： 본발명은활성혈소판보존조성물, 활성혈소판보존방법및 이를이용하여보존된활성혈소판에관한것으로, 보다상세하게는 2가양이온, 염화물, 비타민 B, 셀레늄공급원및 용매를포함하는, 활성혈소판보존조성물; 이를이용한키츠, 활성혈소판보존방법및 이를이용하여보존된활성혈소판에관한것으로, 본발명에의하면, 세포표시인자중 CD61 및 CD62p가양성이면서 PAC-1는음성인혈소판의획득및 보존이가능하다.
摘要翻译：本发明涉及一种保存活性血小板的组合物，活化血小板的保存方法和使用保存方法保存的活化血小板。更具体地说，本发明涉及：用于保存活性血小板的组合物，其包含二价阳离子，氯化物，维生素B，硒源和溶剂; 使用该套件的套件; 活化血小板的保存方法; 并通过使用保存方法保存活化的血小板。根据本发明，可以获得并保存细胞表达参数中具有阳性CD61和CD62p和阴性PAC-1的血小板。

4. 发明授权

KR101426804B1 혈소판 보존 조성물, 이를 포함하는 혈소판 보존 키트 및 이를 이용한 혈소판 보존 방법 有权
标题翻译：血小板保存组合物和用于保存血小板的试剂盒，以及使用其保存血小板的方法
公开(公告)号：KR101426804B1
公开(公告)日：2014-08-05
申请号：KR1020120112061
申请日：2012-10-09
申请人： 김홍승
发明人： 김홍승
IPC分类号： A01N1/00 , A61N1/02 , A61J1/12
摘要： 본 발명은 혈소판을 보존하기 위한 보존 조성물, 이를 포함하는 보존 키트 및 이를 이용하여 혈소판을 장기간 보존하는 방법에 관한 것으로, 보다 상세하게는 혈소판을 4주 이상 장기간 보존하기 위한 보존 조성물, 이를 포함하는 보존 키트 및 이를 이용하여 혈소판을 보존하는 방법에 관한 것이다.

5. 发明授权

KR101290063B1 혈소판 보존 조성물, 이를 포함하는 혈소판 보존 키트 및 이를 이용한 혈소판 보존 방법 有权
标题翻译：板保存组合物和用于保存板的工具箱以及使用该板保护板的方法
公开(公告)号：KR101290063B1
公开(公告)日：2013-07-26
申请号：KR1020120073802
申请日：2012-07-06
申请人： 김홍승
发明人： 김홍승
IPC分类号： A01N1/02 , C12N1/04 , C12N5/02
CPC分类号： A01N1/0226 , C12N1/04 , C12N5/0025
摘要： PURPOSE: A platelet preserving composition, a platelet preserving kit, and a platelet preservation method are provided to store platelet for four or more months and to continuously maintain activated state of platelet. CONSTITUTION: A platelet preserving composition contains adenosine triphosphate (ATP), bivalent cation, chloride, vitamin B, insulin, granulocyte colony-stimulating factor (G-CSF), erythropoietin, and a solvent. A platelet preserving kit has a sealed sterilized container containing the composition, of which internal pressure is regulated to be identical with the external pressure. A platelet preservation method comprises the steps of: mixing blood and an anticoagulant, and centrifuging to obtain buffy coat and plasma; injecting the buffy coat and the plasma to the container; and preserving the container at 35-37 deg. C.
摘要翻译：目的：提供血小板保存组合物，血小板保存试剂盒和血小板保存方法，将血小板储存四个月以上，并持续维持血小板活化状态。构成：血小板保存组合物含有三磷酸腺苷（ATP），二价阳离子，氯化物，维生素B，胰岛素，粒细胞集落刺激因子（G-CSF），促红细胞生成素和溶剂。血小板保存试剂盒具有含有组合物的密封灭菌容器，其内压被调节为与外部压力相同。血小板保存方法包括以下步骤：将血液与抗凝剂混合，离心分离得到血沉棕黄层和血浆; 将血沉棕黄层和等离子体注入容器; 并在35-37度保存容器。 C。

6. 发明授权

KR101290062B1 말초 혈액으로부터 활성화 Ｔ 임파구 세포를 유도하기 위한 배양 조성물, 이를 포함하는 배양 키트 및 이를 이용하여 말초 혈액으로부터 활성화 Ｔ 임파구 세포를 유도하는 방법 有权
标题翻译：培养组合物和用于从外周血培养至活化的T淋巴细胞的培养物包以及使用其从外周血中诱导活化的T淋巴细胞的方法
公开(公告)号：KR101290062B1
公开(公告)日：2013-07-26
申请号：KR1020120073803
申请日：2012-07-06
申请人： 김홍승
发明人： 김홍승
IPC分类号： C12N5/0783 , C12N5/02
CPC分类号： C12N5/0637 , C12N2500/38 , C12N2501/01
摘要： PURPOSE: A culture composition for inducing activated T lymphocytes from peripheral blood, a culture kit, and a method for inducing the activated T lymphocytes using the kit are provided to easily acquire a large amount of T lymphocytes from peripheral blood obtained from adult, and to be used in various fields related to a cell therapeutic agent. CONSTITUTION: A culture composition for inducing activated T lymphocytes from peripheral blood contains adenosine triphosphate (ATP), bivalent cation, chloride, vitamin B, insulin, granulocyte colony-stimulating factors (G-CSF), erythropoietin, and a solvent. A method for inducing the activated T lymphocytes from peripheral blood comprises the steps of: mixing blood and an anticoagulant, and centrifuging to obtain buffy coat and plasma; injecting the buffy coat and the plasma to a sealed sterilized container containing the culture composition, of which internal pressure is regulated to be identical with the external pressure; and putting the container into a low temperature culture machine and culturing.
摘要翻译：目的：提供用于从外周血诱导活化的T淋巴细胞的培养组合物，培养试剂盒和使用试剂盒诱导活化的T淋巴细胞的方法，以容易地从成人获得的外周血中获得大量的T淋巴细胞，并且用于与细胞治疗剂相关的各种领域。构成：用于从外周血诱导活化的T淋巴细胞的培养组合物含有三磷酸腺苷（ATP），二价阳离子，氯化物，维生素B，胰岛素，粒细胞集落刺激因子（G-CSF），促红细胞生成素和溶剂。从外周血诱导活化的T淋巴细胞的方法包括以下步骤：将血液和抗凝血剂混合，离心以获得血沉棕黄层和血浆; 将血沉棕黄层和血浆注射到含有内压与外部压力相同的培养组合物的密封灭菌容器中; 将容器放入低温培养机中进行培养。

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