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    • 65. 发明公开
    • 발효에 의한 대사 산물의 생산 방법 및 소드 유전자를암호화하는 뉴클레오타이드 서열
    • DNA,氨基酸序列,CORYNEFORM BACTERIUM,SHUTTLE VECTOR,在超氧化物歧化酶中增加和生成代谢物
    • KR1020010070021A
    • 2001-07-25
    • KR1020000046638
    • 2000-08-11
    • 에보니크 데구사 게엠베하쌩뜨레 나티오날 데 라 르세르쉬 생띠끄 (씨. 엔. 알. 에스)
    • 메르깡뮤리엘기용바르쉬아르멜마륵스아힘
    • C12N15/77
    • C12N9/0089C12P13/08C12R1/15
    • PURPOSE: To obtain a new preferable recombinant DNA from a bacterium of the genus Corynebacterium, replicable in a coryneform bacterium, having a nucleotide sequence encoding a specific sod gene, useful for producing L-lysine, etc., by fermentation, etc. CONSTITUTION: This new preferable recombinant DNA is replicable in a coryneform bacterium, has at least a nucleotide sequence encoding a sod gene of the formula, is obtained from a bacterium of the genus Corynebacterium and is useful for a method for producing a vitamin and an L-amino acid, especially L-lysine by using a coryneform bacterium for amplifying the sod gene by fermentation. The recombinant DNA is obtained by culturing Corynebacterium melassecola ATCC 17,965, extracting a genome DNA by a routine procedure, constituting a genome DNA bank by using the genome DNA, screening the genome DNA bank by using a partial sequence of a super oxide dismutase gene (sod) as a probe and inserting the obtained sod gene into a vector.
    • 目的:从棒状杆菌属细菌获得新的优选重组DNA,可在棒状细菌中复制,具有编码特异性sod基因的核苷酸序列,通过发酵等方法生产L-赖氨酸等。构成: 这种新的优选的重组DNA可以在棒状细菌中可复制,至少具有编码下式的sod基因的核苷酸序列是从棒状杆菌属的细菌获得的,并且可用于生产维生素和L-氨基酸的方法 酸,特别是L-赖氨酸,通过使用棒状细菌通过发酵来扩增sod基因。 重组DNA通过培养黑曲霉ATCC17965,通过常规方法提取基因组DNA,通过使用基因组DNA构成基因组DNA库,通过使用超氧化物歧化酶基因的部分序列筛选基因组DNA库(sod )作为探针,将获得的sod基因插入载体。