会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 42. 发明公开
    • 재조합체 숙주 세포에서 히알루로난의 제조 방법
    • 在重组宿主细胞中生产羟尿嘧啶的方法
    • KR1020080018965A
    • 2008-02-28
    • KR1020087002156
    • 2002-12-20
    • 노보자임스 바이오폴리머 에이/에스
    • 슬로마알란베하레기네위드너윌리암탕마리아스턴버그데이비드브라운스티븐
    • C12P19/04C12P19/26
    • C12P19/26C12N9/1051C12N15/52
    • The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6- phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP- glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.
    • 本发明涉及透明质酸的制备方法,其包括:(a)在适于产生透明质酸的条件下培养芽孢杆菌属宿主细胞,其中所述芽孢杆菌宿主细胞包含可操作地连接的透明质酸合酶编码序列的核酸构建体 涉及透明质酸合酶编码序列外源的启动子序列; 和(b)从培养基中回收透明质酸。 本发明还涉及编码包含透明质酸合酶基因和UDP-葡萄糖-6-脱氢酶基因的透明质酸合酶操纵子的分离的核酸序列,以及任选地一种或多种选自UDP-葡萄糖焦磷酸化酶基因, UDP-N-乙酰葡糖胺焦磷酸化酶基因和葡萄糖-6-磷酸异构酶基因。 本发明还涉及编码UDP-葡萄糖-6-脱氢酶,UDP-葡萄糖焦磷酸化酶和UDP-N-乙酰氨基葡糖焦磷酸化酶的分离的核酸序列。
    • 48. 发明公开
    • 셀룰로모나스 속 GM13 균주 유래의 신규한 엔도키티나아제유전자
    • 来自CELLULOMONAS SP的新鲜内切酶基因 GM13菌株
    • KR1020020075143A
    • 2002-10-04
    • KR1020010015379
    • 2001-03-23
    • 아미코젠주식회사
    • 신용철전영중정경화최연진
    • C12N15/56
    • C12N9/2442C12P19/26C12Y302/01014
    • PURPOSE: Provided is the nucleotide sequence of a novel endochitinase gene derived from Cellulomonas sp. GM13 strain. Also, provided is the amino acid sequence translated therefrom. Therefore, chitin oligosaccharide and NAG can be manufactured from chitin by using an endochitinase encoded by the novel endochitinase gene. CONSTITUTION: The endochitinase gene is isolated from a Cellulomonas sp. GM13 strain by genetic recombination. Its nucleotide sequence and amino acid sequence are represented by SEQ ID NO:1 and SEQ ID NO:2 respectively. An endochitinase is manufactured by culturing a transformant which is transformed with an expression vector including the endochitinase gene.
    • 目的:提供来源于纤维单胞菌属的新型内切壳酶基因的核苷酸序列。 GM13菌株。 此外,提供了从其翻译的氨基酸序列。 因此,几丁质寡糖和NAG可以通过使用由新型内切素酶基因编码的内切素酶由几丁质制备。 构成:内切素酶基因从纤维单胞菌属中分离出来。 GM13菌株通过遗传重组。 其核苷酸序列和氨基酸序列分别由SEQ ID NO:1和SEQ ID NO:2表示。 通过培养用包含内切素酶基因的表达载体转化的转化体来制造内切壳蛋白酶。
    • 49. 发明公开
    • 키토산 및 키토산 제조 방법
    • CHITOSAN及其制剂
    • KR1020010091847A
    • 2001-10-23
    • KR1020000042436
    • 2000-07-24
    • 카르길,인코포레이티드
    • 판,웨이유보흘만,존에이.트링클,제임스알.스테인크,제임스디.황기오헨닝,조셉피.
    • C08B37/08
    • C12P19/26A23L29/275A61K31/722C08B37/003Y10S435/911Y10S435/917Y10S435/921Y10T442/2525
    • PURPOSE: Chitosan and a method for preparing the chitosan from a biomass of microorganism are provided, to improve the solubility in water and to reduce the viscosity of chitosan. CONSTITUTION: The chitosan which contains a material derived from a biomass of microorganism, is characterized by containing a material making 85 wt% or more of the N-acetyl group of chitin to be deacetylated. Preferably the chitosan comprises further a material containing 0.05% or less of lime. The 1 wt% chitosan solution has a viscosity less than 25 cP, preferably less than 15 cP in 1% aqueous acetic acid solution at a temperature of 25 deg.C. The method comprises the steps of reacting a biomass of chitin in 25% or more alkali hydroxide solution at a temperature above 95 deg.C for at least 10 hours for converting chitin into chitosan; and separating the chitosan from the solution.
    • 目的:提供壳聚糖和从微生物生物质制备壳聚糖的方法,以提高在水中的溶解度和降低壳聚糖的粘度。 构成:含有源自微生物生物质的材料的壳聚糖的特征在于含有85重量%以上的脱乙酰化的甲壳质N-乙酰基的物质。 优选地,壳聚糖还包含含有0.05%或更少的石灰的材料。 1重量%的壳聚糖溶液在25℃的温度下,在1%乙酸水溶液中的粘度小于25cP,优选小于15cP。 该方法包括以下步骤:将甲壳素的生物质在25℃或更高的碱金属氢氧化物溶液中在高于95℃的温度下反应至少10小时,以将壳多糖转化成壳聚糖; 并从溶液中分离脱乙酰壳多糖。