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41. 发明公开

KR1020090097440A 들깻잎으로부터 γ―ＧＣＳ의 활성을 증가시키는카페인산을 제조하는 방법, 그로부터 제조된 카페인산, 및상기 카페인산을 포함하는 식품 조성물 및 약학 조성물 有权
标题翻译：用于制备改善γ-GCS活性的CAFFEIC的方法，其制备的CAFFEIC和食品组合物以及包含CAFFEIC的药物组合物
公开(公告)号：KR1020090097440A
公开(公告)日：2009-09-16
申请号：KR1020080022574
申请日：2008-03-11
申请人： 고려대학교 산학협력단
发明人： 이광원 , 이현순 , 박호영
IPC分类号： A23L1/29 , A23L2/00 , A23L1/212
CPC分类号： A23L33/00 , A23L2/00 , A23L19/00 , A23V2002/00 , A23V2200/30 , A23V2250/21 , A61K31/192 , A61K36/535
摘要： A method for producing caffeic acid improving gamma-GCS activity from green perilla leaves is provided to secure an improvement to liver and antioxidant effect at a little cost. A method for producing caffeic acid improving gamma-GCS activity from green perilla leaves comprises: a first step of reflux-extracting dried green perilla leaves in distilled water; a second step of centrifuging the extract to obtain supernate from it; a third step of filtering the supernate and vacuum-concentrating and freeze-drying the filtrate in order to obtain powder; a fourth step of extracting the powder using a plurality of extraction solvents in sequential order; and a fifth step of separating and refining the fractions showing high gamma-GCS activity. In the first step, 100g of the dried green perilla leaves are extracted in 0.8-1.2L of distilled water at 100-110°C for 3-4 hours twice. In the second step, the centrifugation is performed at 4°C and 7500rpm for 25-30 minutes. The extraction solvents are a first, second, third and fourth extraction solvent.
摘要翻译：提供了一种从绿色紫苏叶提高咖啡酸提高γ-GCS活性的方法，以确保肝脏和抗氧化作用的改善。一种从青紫苏叶提取咖啡酸提高γ-GCS活性的方法，包括：在蒸馏水中回收提取干燥的紫苏叶片的第一步; 离心提取物以从其中获得上清液的第二步骤; 过滤上清液并对滤液进行真空浓缩和冷冻干燥以获得粉末的第三步骤; 第四步骤，使用多个提取溶剂依次提取粉末; 以及分离和精制显示高γ-GCS活性的级分的第五步骤。在第一步中，将100g干黄绿色叶子在0.8-1.2L蒸馏水中在100-110℃下萃取3-4小时。在第二步中，离心在4℃和7500rpm下进行25-30分钟。萃取溶剂是第一，第二，第三和第四萃取溶剂。

42. 发明公开

KR1020070074763A 식물 유효성분의 추출정제방법 失效
标题翻译：植物有效成分的提取和纯化方法
公开(公告)号：KR1020070074763A
公开(公告)日：2007-07-18
申请号：KR1020060002721
申请日：2006-01-10
申请人： 고려대학교 산학협력단
发明人： 이광원 , 이현순 , 홍충의 , 정성훈
IPC分类号： A61K36/68 , A61P17/18 , A61P35/00
CPC分类号： A61K36/68 , A61K2236/33 , A61K2236/51 , A61K2236/53
摘要： An extraction and purification method for an effective component of a plant is provided to reduce the use amounts of extraction solvent and column resin by performing the extraction and purification processes once only, thereby reducing extraction time and enhancing economic efficiency of the extraction. The extraction and purification method for the effective component of the plant comprises the steps of: solubilizing plant powder in ethanol, heating the mixture solution with a reflux at boiling point for 2-4 hours, centrifuging the plant extract at 2000-3000 rpm for 7-15 minutes, and collecting the supernatant to prepare the crude extract; drying the crude extract through vacuum-drying or vacuum-drying and freeze-drying, and dissolving the dried crude extract in water; and subjecting the crude extract solution to ion exchange resin and eluting it with 0-100% ethanol gradients to obtain fractions, wherein the plant is Plantago asiatica Decaisne and the effective component of plant is plantamajoside.
摘要翻译：提供植物有效成分的提取和提纯方法，通过一次进行提取和纯化过程来减少萃取溶剂和柱状树脂的使用量，从而减少萃取时间并提高萃取的经济效率。植物有效成分的提取和纯化方法包括以下步骤：将植物粉末溶于乙醇中，加热回流沸点为2-4小时的混合溶液，以2000-3000 rpm离心植物提取物7 -15分钟，收集上清液制备粗提物; 通过真空干燥或真空干燥和冷冻干燥干燥粗提取物，并将干燥的粗提取物溶解在水中; 并将粗提物溶液进行离子交换树脂，用0-100％乙醇梯度洗脱，得到部分，其中植物是车前草，并且植物的有效成分是植物苷。

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