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    • 33. 实用新型
    • 항공등화용 코어장치
    • 用于在机场右侧钻核心的装置
    • KR200398005Y1
    • 2005-10-12
    • KR2020050019512
    • 2005-07-05
    • 황재곤김희태
    • 황재곤김희태
    • B64F1/18E01C23/16
    • 본 고안은 활주로에 매설된 캔의 직 상방에 코어를 정확하고 정밀하게 형성할 수 있는 항공등화용 코어장치에 관한 것이다.
      이를 위해 본 고안에서는 항공등화를 설치하기 위해 콘크리트 표면에 코어를 형성하는 것에 있어서, 구멍을 형성하고 캐스터를 구비한 베이스 플레이트와; 상기 베이스 플레이트에 설치하되 손잡이를 갖는 다수의 나사봉과; 상기 나사봉에 설치한 핸들과; 상기 다수의 나사봉을 함께 회동되도록 하는 연동장치와; 상기 나사봉의 상부에 설치한 상부 플레이트와; 상기 나사봉에 설치하여 나사봉의 회동에 의해 나사봉을 따라 승하강되는 승하강 플레이트와; 상기 승하강 플레이트에 탑재되는 엔진과; 상기 엔진의 구동력에 의해 회전하되 상기 승하강 플레이트에 하향되게 설치한 코어드릴을 포함하여 구성한 것을 특징으로 한다.
      따라서 본 고안은 코어드릴이 요동되거나 한쪽으로 기울어지지 않고 직 하방으로 하강되면서 정밀하고 정확하게 코어를 형성할 수 있는 매우 유용한 효과가 있으며, 코어 형성과 관련된 작업 불량률을 근본적으로 방지하여 작업시간을 크게 단축할 수 있는 매우 유용한 효과가 있다.
    • 35. 发明授权
    • 다운증후군 관련 호르몬 측정 방법 및 측정 키트
    • 다운증후군관련호르몬측정방법및측정키트
    • KR100401390B1
    • 2003-10-17
    • KR1020000063386
    • 2000-10-27
    • 김희태
    • 김희태윤용달
    • A61B5/145
    • PURPOSE: A method for diagnosing Down syndrome and a diagnosis kit thereof are provided to simultaneously analyze concentrations of four Down syndrome hormone at a space and confirm Down syndrome of a fetus fast, accurately and conveniently. CONSTITUTION: A Down syndrome diagnosis kit comprises a body(30), blood sample injecting unit(80), a second antibody storage(50) and a colorimetic indicator storage(70). The body(30) is made of transparent material has a capillary(31) branched into four tubes. The four tubes have reaction spaces(110a,120a,130a,140a) on their inner surfaces, respectively, to form an AFP analysis capillary(140) containing an antibody against the AFP, a hCG analysis capillary(130) containing an antibody against the hCG, a uE3 analysis capillary(120) containing an antibody against the uE3, and an inhibin A analysis capillary(130) containing an antibody against the inhibin A. The blood sample injecting unit(80) injects sample into the capillary(31). The second antibody storage(50) stores second antibody solution containing colorimetic enzyme to be injected into the capillary(31) for reaction with the colorimetic indicator from the colorimetic indicator storage(70).
    • 目的:提供一种诊断唐氏综合征的方法及其诊断试剂盒,用于同时分析一个空间处的四种唐氏综合征的浓度,并快速,准确和方便地确认胎儿唐氏综合征。 构成:唐氏综合征诊断试剂盒包括身体(30),血液样品注射单元(80),第二抗体储存器(50)和比色指示剂储存器(70)。 主体(30)由透明材料制成,具有分成四个管的毛细管(31)。 四个管分别在其内表面上具有反应空间(110a,120a,130a,140a)以形成含有针对AFP的抗体的AFP分析毛细管(140),含有针对AFP的抗体的hCG分析毛细管 hCG,含有针对uE3的抗体的uE3分析毛细管(120)和含有针对抑制素A的抗体的抑制素A分析毛细管(130)。血液样品注入单元(80)将样品注入毛细管(31)中。 第二抗体储存器(50)储存含有将被注入毛细管(31)中的比色学酶的第二抗体溶液,以与来自比色指示器储存器(70)的比色指示剂反应。
    • 36. 发明公开
    • 핵산 증폭 산물-프로브간의 혼성화반응 효율을 증대시킨핵산 검출 방법
    • 具有改进PCR产物探针的杂交效率的核酸检测方法
    • KR1020020032755A
    • 2002-05-04
    • KR1020000063387
    • 2000-10-27
    • 김희태
    • 김희태최영길이영훈한명수
    • C12Q1/68
    • PURPOSE: A nucleic acid detecting method with improved hybridization efficiency of PCR product-probe is provided, thereby inhibiting PCR products from binding together and increasing hybridization efficiency between PCR product and probe. Therefore, the detection of nucleic acid can be more correct. CONSTITUTION: The nucleic acid detecting method using improved hybridization efficiency of PCR product-probe comprises the steps of: inserting nucleic acid sample into a vessel containing a PCR solution comprising any one of sense and anti-sense primer fixed magnetic bead and a fluorescence-labeled primer; amplifying the nucleic acid sample at PCR temperature; heat denaturing the amplified double strand PCR product into single strand PCR product; fixing nucleic acid synthesized by a primer which is fixed on the magnetic bead on the inner side of the vessel using magnetic power; hybridizing the remaining PCR solution with a support having a probe capable of specifically binding with a target protein; removing and washing the unbound PCR product and solution; and determining the fluorescence level of PCR product-probe hybridization on the support.
    • 目的:提供PCR产物探针杂交效率提高的核酸检测方法,从而抑制PCR产物结合在一起,提高PCR产物与探针之间的杂交效率。 因此,核酸的检测可以更正确。 构成:使用改进的PCR产物探针的杂交效率的核酸检测方法包括以下步骤:将核酸样品插入含有包含有义和反义引物固定磁珠和荧光标记的任意一个的PCR溶液的容器中 底漆; 在PCR温度下扩增核酸样品; 将扩增的双链PCR产物热变性为单链PCR产物; 通过使用磁力固定在容器内侧的磁珠上的底漆合成的核酸; 将剩余的PCR溶液与具有能够与靶蛋白特异性结合的探针的载体杂交; 去除和洗涤未结合的PCR产物和溶液; 并确定载体上PCR产物 - 探针杂交的荧光水平。
    • 37. 发明公开
    • 핵산 검출 방법, 이를 위한 키트 및 이 키트를 구동시키기위한 구동장치
    • 核酸检测方法,其使用的套件和用于操作该套件的装置
    • KR1020020032750A
    • 2002-05-04
    • KR1020000063382
    • 2000-10-27
    • 김희태
    • 김희태최영길이영훈
    • C12Q1/68
    • PURPOSE: A nucleic acid-detecting method, a kit used therefor, and a device for operating the same kit are provided, thereby detecting a specified nucleic acid rapidly and simply. CONSTITUTION: The nucleic acid-detecting method comprises the steps of: mixing a blood sample with a magnetic bead having a target cell antibody, to bind the target cell in the blood sample with the magnetic beads; introducing the blood sample into a capillary tube; separating the target cell by fixing it to the inner side of the capillary tube using magnetic power; removing unbound sample from the capillary tube and washing it; introducing PCR solution containing a fluorescence-labeled primer into the capillary tube and heating it to 94 deg.C for 5 minutes; amplifying the solution by PCR at 94 deg.C, 55 deg.C and 72 deg.C; heating the amplified nucleic acid sample at 94 deg.C for 5 minutes to denature the PCR product; binding the denatured PCR product with a probe; removing unbound product and washing it; and determining the fluorescence level.
    • 目的:提供核酸检测方法,用于其的试剂盒和用于操作相同试剂盒的装置,从而快速且简单地检测指定的核酸。 构成:核酸检测方法包括以下步骤:将血液样品与具有靶细胞抗体的磁珠混合,将血液样品中的靶细胞与磁珠结合; 将血液样品引入毛细管中; 通过使用磁力将其固定到毛细管的内侧来分离靶细胞; 从毛细管中除去未结合的样品并进行洗涤; 将含有荧光标记的引物的PCR溶液引入毛细管中并加热至94℃5分钟; 在94℃,55摄氏度和72摄氏度下通过PCR扩增溶液; 将扩增的核酸样品在94℃加热5分钟以使PCR产物变性; 将变性PCR产物与探针结合; 清除未结合的产品并清洗; 并确定荧光水平。
    • 40. 发明公开
    • 여러 종류의 디엔에이 프로브가 부착된 마이크로 웰 플레이트를 사용하여 핵산 시료내 특정 핵산을 검출하는 방법
    • 使用具有各种DNA探针的微孔板从核酸样品中检测特异性核酸的方法
    • KR1020010016720A
    • 2001-03-05
    • KR1019990031754
    • 1999-08-02
    • 김희태
    • 김희태
    • C12Q1/68
    • C12Q1/708C12Q1/6837C12Q2565/625
    • PURPOSE: A method for detecting specific nucleic acids from a nucleic acid sample is provided which uses a micro well plate having various DNA probes in order to treat several samples at the same time. CONSTITUTION: A method for detecting specific nucleic acids from a nucleic acid sample is characterized by the next steps of: i) attaching various, specific DNA probes into a tube of a micro well plate; ii) adding nucleic acids to be measured into the tube and heating the nucleic acids for degeneration; iii) reacting the nucleic acids degenerated during step (ii) with DNA probes already attached on the tube; iv) washing non-specific nucleic acids not having reacted in step (ii) to remove the nucleic acids; and v) measuring the results of a reaction between the nucleic acids of step (ii) and DNA probes in the tube.
    • 目的:提供一种用于从核酸样品中检测特异性核酸的方法,其使用具有各种DNA探针的微孔板,以同时处理多个样品。 构成:用于从核酸样品检测特异性核酸的方法的特征在于以下步骤:i)将各种特异性DNA探针连接到微孔板的管中; ii)将待测量的核酸加入到管中并加热核酸进行退化; iii)使步骤(ii)中退化的核酸与已经附着在管上的DNA探针反应; iv)洗涤在步骤(ii)中没有反应以除去核酸的非特异性核酸; 和v)测量步骤(ii)的核酸和管中的DNA探针之间的反应结果。