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    • 22. 发明授权
    • 신규 레반슈크라제를 이용한 레반의 생산방법
    • 신규레반슈크라제를이용한레반의생산방
    • KR100420313B1
    • 2004-03-03
    • KR1020010041532
    • 2001-07-11
    • 주식회사 리얼바이오텍한국생명공학연구원
    • 김철호장은경송기방이상기강순아장기효고이삭
    • C12N1/20
    • PURPOSE: A process for producing levan using novel levansucrase is provided, thereby enzymatically and cheaply mass producing the levan from sugar. CONSTITUTION: A microorganism, Pseudomonas aurantiaca S-4380(KCTC 0943BP), is capable of producing the levan having 6% of a β-(2,1) bond branch. The levansucrase isolated from Pseudomonas aurantiaca S-4380(KCTC 0943BP) has the amino acid sequence of SEQ ID NO: 1. The levansucrase isolated from Pseudomonas aurantiaca S-4380(KCTC 0943BP) may also have the nucleotide sequence of SEQ ID NO: 2. A recombinant plasmid pUPLK1 is constructed by adding a recombinant secretion signal sequence represented by SEQ ID NO: 3 to the N-terminal of the nucleotide sequence of SEQ ID NO: 2. A transformant E. coli JM109/pUPLK1(KCTC 0942BP) is produced by transforming E. coli JM109 with the recombinant plasmid pUPLK1. The levansucrase is expressed from the transformant E. coli JM109/pUPLK1(KCTC 0942BP), wherein the optimal temperature of the levansucrase expression is 30 to 35 deg. C.
    • 目的:提供一种使用新型果聚糖蔗糖酶生产果聚糖的方法,由此通过酶从糖中大量生产果聚糖。 构成:微生物Pseudomonas aurantiaca S-4380(KCTC 0943BP)能够产生具有6%β( - ) - (2,1)键分支的果聚糖。 从Pseudomonas aurantiaca S-4380(KCTC 0943BP)分离的左聚蔗糖酶具有SEQ ID NO:1的氨基酸序列。从Pseudomonas aurantiaca S-4380(KCTC 0943BP)分离的左聚蔗糖酶也可具有SEQ ID NO:2的核苷酸序列 通过将SEQ ID NO:3代表的重组分泌信号序列加到SEQ ID NO:2的核苷酸序列的N端构建重组质粒pUPLK1。转化体大肠杆菌JM109 / pUPLK1(KCTC 0942BP)为 通过用重组质粒pUPLK1转化大肠杆菌JM109产生。 左聚蔗糖酶由转化体大肠杆菌JM109 / pUPLK1(KCTC 0942BP)表达,其中果聚糖蔗糖酶表达的最适温度为30至35℃。 C。
    • 25. 发明公开
    • 신규 레반슈크라제를 이용한 레반의 생산방법
    • 使用新型LEVANUCRASE生产LEVAN的工艺
    • KR1020030005996A
    • 2003-01-23
    • KR1020010041532
    • 2001-07-11
    • 주식회사 리얼바이오텍한국생명공학연구원
    • 김철호장은경송기방이상기강순아장기효고이삭
    • C12N1/20
    • PURPOSE: A process for producing levan using novel levansucrase is provided, thereby enzymatically and cheaply mass producing the levan from sugar. CONSTITUTION: A microorganism, Pseudomonas aurantiaca S-4380(KCTC 0943BP), is capable of producing the levan having 6% of a β-(2,1) bond branch. The levansucrase isolated from Pseudomonas aurantiaca S-4380(KCTC 0943BP) has the amino acid sequence of SEQ ID NO: 1. The levansucrase isolated from Pseudomonas aurantiaca S-4380(KCTC 0943BP) may also have the nucleotide sequence of SEQ ID NO: 2. A recombinant plasmid pUPLK1 is constructed by adding a recombinant secretion signal sequence represented by SEQ ID NO: 3 to the N-terminal of the nucleotide sequence of SEQ ID NO: 2. A transformant E. coli JM109/pUPLK1(KCTC 0942BP) is produced by transforming E. coli JM109 with the recombinant plasmid pUPLK1. The levansucrase is expressed from the transformant E. coli JM109/pUPLK1(KCTC 0942BP), wherein the optimal temperature of the levansucrase expression is 30 to 35 deg. C.
    • 目的:提供一种使用新型左倍半胱氨酸蔗糖酶生产levan的方法,从而从糖中高效廉价地大量生产levan。 构成:一种微生物,Pseudomonas aurantiaca S-4380(KCTC 0943BP)能够产生具有6%β-(2,1)键分支的果糖。 从黄花假单胞菌S-4380(KCTC 0943BP)分离的左旋糖蔗糖酶具有SEQ ID NO:1的氨基酸序列。从假单胞菌S-4380(KCTC 0943BP)分离的左旋糖蔗糖酶也可以具有SEQ ID NO:2的核苷酸序列 通过将SEQ ID NO:3所示的重组分泌信号序列添加到SEQ ID NO:2的核苷酸序列的N末端构建重组质粒pUPLK1。转化体大肠杆菌JM109 / pUPLK1(KCTC 0942BP)是 通过用重组质粒pUPLK1转化大肠杆菌JM109产生。 左旋糖蔗糖酶由转化体大肠杆菌JM109 / pUPLK1(KCTC 0942BP)表达,其中左半乳糖蔗糖酶表达的最适温度为30-35℃。 C。
    • 26. 发明公开
    • 고정화된 레반슈크라제 또는 유기용매 처리된 균체를이용한 저분자량 레반의 제조방법
    • 使用固定化液晶显微镜或有机溶剂处理细胞生产低分子量LEVAN的方法
    • KR1020010068479A
    • 2001-07-23
    • KR1020000000417
    • 2000-01-06
    • 한국생명공학연구원
    • 이상기송기방장기효김철호정봉현
    • C12P19/04
    • PURPOSE: The methods for producing low molecular weight of levan using immobilized levansucrase or organic solvent treated cells are provided, thereby the low molecular weight of levan can be efficiently and cheaply produced. CONSTITUTION: The low molecular weight of levan is produced by the steps of: adding magnetic iron oxide as a resin into the mixture of TiCl4 and water and agitating it; adding ammonia solution into the mixture and agitating it; washing the mixture with acetic acid buffer solution; mixing the resins with levansucrase and agitating it at 4 deg. C for 30 to 16 hours; adding the immobilized levansucrase into medium containing sucrose as a substrate; incubating it at 0 to 37 deg. C for 20 to 100 hours. Alternatively, the low molecular weight of levan is produced by incubating the organic solvent such as toluene treated cells in medium containing sucrose as a substrate.
    • 目的:提供使用固定化的左旋糖蔗糖酶或有机溶剂处理的细胞生产低分子量levan的方法,从而可以有效且廉价地生产levan的低分子量。 构成:通过以下步骤产生levan的低分子量:将磁性氧化铁作为树脂添加到TiCl 4和水的混合物中并搅拌; 将氨溶液加入到混合物中并搅拌; 用乙酸缓冲溶液洗涤混合物; 将树脂与左半乳蔗糖酶混合并在4度搅拌。 C为30〜16小时; 将固定化的左旋蔗糖酶加入含有蔗糖作为底物的培养基中; 在0至37度孵育。 C为20至100小时。 或者,通过将有机溶剂如甲苯处理的细胞在含有蔗糖作为底物的培养基中孵育来制备levan的低分子量。