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    • 11. 发明授权
    • 어병방제용 황토슬러리 조성물, 이를 이용한 어병 방제 및 예방방법
    • 使用红色粘土浆液控制和预防鱼类疾病的组合物及其制造方法
    • KR101584918B1
    • 2016-01-15
    • KR1020140140609
    • 2014-10-17
    • 동국대학교 산학협력단
    • 박정극윤문영성정석배승철김민
    • A61K33/00A61P31/04A61P33/00
    • 본발명은 1) 황토 60 내지 90 중량%에대하여가성소다(sodium hydroxide, NaOH) 10 내지 40 중량%을넣고혼합하는단계; 2) 상기혼합물을흑연도가니가장착된용해로에서 1100℃내지 1200℃로용융시키는단계; 3) 상기용융물을냉각시키는단계; 4) 상기냉각물을분쇄기를이용하여분쇄시키는단계; 5) 상기분쇄물에물을첨가한후 2차분쇄하여황토슬러리를제조하는단계; 및 6) 상기황토슬러리에황산을첨가하여중화시키는단계; 7) 상기중화된황토슬러리를양식장사육수에첨가하는단계;를포함하는황토슬러리를이용한어병방제및 예방방법에관한것이다.
    • 本发明涉及一种利用红泥浆控制和预防鱼病的方法。 该方法包括以下步骤:1)相对于60至90重量%的红土,将10至40重量%的氢氧化钠(NaOH)相互混合并混合; 2)在安装有石墨坩埚的熔炉中在1100〜1200℃的温度下熔融混合物; 3)冷却熔融材料; 4)使用破碎机破碎冷却材料; 5)通过在粉碎物料中加入水后二次破碎制造红泥浆; 6)通过向红泥浆中加入硫酸中和; 和7)将中和的红粘土浆添加到养殖场的养殖水中。
    • 14. 发明公开
    • 극저주파 전자기장을 이용한 유전자 과발현 방법
    • 使用极低频电磁场的超表达基因方法
    • KR1020130061978A
    • 2013-06-12
    • KR1020110128330
    • 2011-12-02
    • 동국대학교 산학협력단
    • 성정석박정극심진한고건
    • C12N15/85C12N13/00C12P21/00C07K14/195
    • PURPOSE: A method for gene overexpression is provided to promote expression of a target gene thereby producing a large amount of protein, and to regulate gene expression by a non-invasive method and specific mechanism. CONSTITUTION: A method for gene overexpression comprises: a step of transforming a mammalian cell by a vector containing a wave-sensitive promoter and a gene and preparing a transformed cell; and a step of culturing the transformed cells by 50 Hz of extremely low frequencies and 0.1mT of electromagnetic fields. The wave-sensitive promoter is human telomerase transcriptase. The gene is a heterogeneous gene. The mammalian cells are human kidney cell line, HEK293T. Proteins are prepared by an overexpression method. The protein is Staphylococcus SEB.
    • 目的:提供一种基因过表达方法,以促进靶基因的表达,从而产生大量蛋白质,并通过非侵入性方法和特异性机制调控基因表达。 构成:用于基因过表达的方法包括:通过含有波动敏感启动子和基因的载体转化哺乳动物细胞并制备转化细胞的步骤; 以及通过50Hz的极低频和0.1mT的电磁场培养转化的细胞的步骤。 波敏感启动子是人端粒酶转录酶。 该基因是异源基因。 哺乳动物细胞是人肾细胞系,HEK293T。 蛋白质通过过表达方法制备。 蛋白质是葡萄球菌SEB。
    • 15. 发明公开
    • 천연 칼슘 킬레이팅제를 이용한 골형성 단백질 고정화 방법
    • 生物材料自然螯合材料的生长因子固定方法
    • KR1020120083667A
    • 2012-07-26
    • KR1020110004935
    • 2011-01-18
    • 동국대학교 산학협력단
    • 서영권박정극김성현한미정홍국선윤희훈이종호정현석
    • A61L27/34A61L27/22A61L27/06A61L27/54A61L27/36A61F2/02
    • A61L27/34A61F2/02A61L27/06A61L27/22A61L27/365A61L27/54
    • PURPOSE: An immobilization method of bone morphogenetic proteins is provided to minimize immune reactions after bone graft by using a natural calcium chelating agent, to have excellent engraftment rate, and to improve cell suitability, biological suitability, and hydrophilicity, thereby inducing a fast bone growth. CONSTITUTION: An immobilization method of bone morphogenetic proteins comprises: a step of forming a coating layer on the surface of an implant; a step of combining chitosan or alginate as a natural calcium chelating agent on the coating layer; a step of immobilization of bone morphogenetic proteins by using diphenylphosphoryl azide, hexamethylene isocyanate, succinimide, carbodiimide glutaraldehyde or ethyl dimethylaminopropyl carbodiimide-crosslinked reagent; a step of forming hydroxyapatite or beta-tricalcium phosphate coated on the surface of titanium. The bone morphogenetic protein is selected from a group consisting of BMP-2, BMP-4, and BMP-12.
    • 目的:提供骨形态发生蛋白的固定化方法,通过使用天然钙螯合剂使骨移植后的免疫反应最小化,植入率优异,提高细胞适应性,生物适应性和亲水性,从而诱导快速骨生长 。 构成:骨形态发生蛋白的固定化方法包括:在植入物的表面上形成被覆层的步骤; 将壳聚糖或藻酸盐作为天然钙螯合剂结合在涂层上的步骤; 使用二苯基磷酰基叠氮化物,六亚甲基异氰酸酯,琥珀酰亚胺,碳二亚胺戊二醛或乙基二甲基氨基丙基碳二亚胺交联试剂来固定骨形态发生蛋白的步骤; 在钛表面上形成羟磷灰石或磷酸三钙的步骤。 骨形态发生蛋白选自BMP-2,BMP-4和BMP-12。
    • 16. 发明公开
    • 간세포의 제조방법 및 이로부터 제조된 간세포
    • 制备肝细胞的方法和制备的肝细胞
    • KR1020120007849A
    • 2012-01-25
    • KR1020100068606
    • 2010-07-15
    • 동국대학교 산학협력단라이프리버 주식회사
    • 박정극윤희훈김효은장인근이두훈
    • C12N5/077C12N5/0775C12N5/02C07K14/475
    • PURPOSE: A method for preparing hepatocytes with liver-specific function using MSC(mesenchymal stem cells) is provided to be used as hepatocyte source for evaluating toxicity. CONSTITUTION: A method for preparing hepatocytes comprises: a step of treating MSC(mesenchymal stem cells) with cell growth factor in a serum-free medium containing a serum substitute to induce hepatocytes; a step of treating cell growth factors, cytokines, and biochemicals in the serum-free medium to mature the hepatocytes. The mesenchymal stem cells are derived from bone marrow, fat, and umbilical cord blood. The serum substitute is knockout TMserum replacement. The cell growth factor includes FGF-1(fibroblast growth factor), FGF-2, FGF-4, and mixture thereof. The biochemical includes dexamethasone and DMSO.
    • 目的:使用MSC(间充质干细胞)制备具有肝脏特异功能的肝细胞的方法用作肝细胞来评估毒性。 构成:制备肝细胞的方法包括:在含有血清替代物的无血清培养基中用细胞生长因子处理MSC(间充质干细胞)以诱导肝细胞的步骤; 在无血清培养基中处理细胞生长因子,细胞因子和生物化学物质以使肝细胞成熟的步骤。 间充质干细胞衍生自骨髓,脂肪和脐带血。 血清替代品是淘汰TMserum替代品。 细胞生长因子包括FGF-1(成纤维细胞生长因子),FGF-2,FGF-4及其混合物。 生物化学包括地塞米松和DMSO。
    • 18. 发明公开
    • 토양 친화 미생물의 자체 순환 시스템을 이용한 식물성장촉진방법
    • 利用土壤微生物自循环系统改善植物生长的方法
    • KR1020080059543A
    • 2008-06-30
    • KR1020080057981
    • 2008-06-19
    • 동국대학교 산학협력단
    • 박정극윤문영김보미
    • A01N63/00A01N63/02C12N1/00
    • A01N63/02A01N25/02A01N25/04A01N25/08A01N25/14C12N1/20C12R1/05
    • A method for isolating soil-friendly microorganisms from a cultivated land is provided to isolate the microorganisms with excellent soil-affinity from the cultivated land to increase productivity of an environment-friendly agricultural product by spraying a complex culture of the microorganisms onto the cultivated land, thereby promoting the plant growth. A method for isolating soil-friendly microorganisms from a cultivated land comprises the steps of: (a) mixing a soil sample of a land for cultivating a plant with saline solution; (b) adding a soil sample of the same land to a minimum culture medium to prepare a minimum culture medium for isolating soil-friendly microorganisms; (c) inoculating the mixture obtained from the step(b) into the minimum culture medium prepared from the step(b); (d) culturing an inoculation solution obtained from the step(c) at a temperature of 25-30 deg.C for 15-20 days; (e) after diluting a culture solution obtained from the step(d) with saline solution, smearing a culture medium for isolation with the diluted solution and culturing it at a temperature of 25-30 deg.C for 5-7 days; and (f) isolating colonies formed on the culture medium as the soil friendly microorganisms of the corresponding land, wherein the minimum culture medium for isolation includes 0.1-0.2 weight/volume% of NH4Cl, 0.44-0.54 weight/volume% of K2HPO4, 0.4-0.5 weight/volume% of NaH2PO4.2H2O, 0.05-0.06 weight/volume% of MgSO4.7H2O, 0.0003-0.0004 weight/volume% of CaCl2.2H2O, 0.0001-0.0002 weight/volume% of FeSO4.7H2O, 0.0001-0.0002 weight/volume% of MnCl2.4H2O, 0.00001-0.00002 weight/volume% of CoCl2.6H2O, 0.00001-0.00002 weight/volume% of Na2MoO4.2H2O and 0.00004-0.00005 weight/volume% of monohydrated citric acid and the culture medium for isolation includes 0.4-0.5 wt.% of peptone, 0.2-0.3 wt.% of a beef extract and 1.0-1.5 wt.% of agar.
    • 提供一种从耕地分离土壤微生物的方法,通过将微生物的复杂培养物喷洒到耕地上,从耕地中分离具有优良土壤亲和力的微生物,从而提高环境友好型农产品的生产力, 从而促进植物生长。 从耕地分离土壤微生物的方法包括以下步骤:(a)将用于培养植物的土地土壤样品与盐溶液混合; (b)将相同土地的土壤样品添加到最小培养基中,以制备用于分离土壤友好微生物的最小培养基; (c)将从步骤(b)获得的混合物接种到由步骤(b)制备的最小培养基中; (d)在25-30℃的温度下培养由步骤(c)获得的接种溶液15-20天; (e)在用盐水溶液稀释步骤(d)得到的培养液后,用稀释液涂抹培养基进行分离,并在25-30℃的温度下培养5-7天; 和(f)分离培养基上形成的菌落作为相应土地的土壤友好的微生物,其中用于分离的最小培养基包括0.1-0.2重量/体积%的NH 4 Cl,0.44-0.54重量/体积%的K 2 HPO 4,0.4 -0.5重量/体积%的NaH 2 PO 4·2H 2 O,0.05-0.06重量/体积%的MgSO 4·7H 2 O,0.0003-0.0004重量/体积%的CaCl 2·2H 2 O,0.0001-0.0002重量/体积%的FeSO 4·7H 2 O,0.0001-0.0002 重量/体积%的MnCl 2·4H 2 O,0.00001-0.00002重量/体积%的CoCl 2·6H 2 O,0.00001-0.00002重量/体积%的Na 2 MoO 4·2H 2 O和0.0000重量/体积%的一水合柠檬酸和用于分离的培养基 包括0.4-0.5重量%的胨,0.2-0.3重量%的牛肉提取物和1.0-1.5重量%的琼脂。
    • 19. 发明授权
    • 천연 광물 유래의 식물 탄저병 방제용 조성물 및 이를이용한 방제 방법
    • 用于控制含有天然矿物成分的烟草醇的组合物和使用其控制蒽醌的方法
    • KR100768478B1
    • 2007-10-19
    • KR1020060052186
    • 2006-06-09
    • 동국대학교 산학협력단
    • 박정극윤문영김보미
    • A01N61/00
    • A01N59/00A01N25/12A01N59/06A01N59/16A01N59/26
    • A plant anthracnose controlling composition comprising a natural mineral as an effective ingredient is provided to control the anthracnose in effective and environmental-friendly method, promote the growth of crops and restore contaminated soil. A composition for controlling anthracnose comprises a mixture consisting of 40.0-60.0 wt.% of SiO2, 10.0-25.0 wt.% of Al2O3, 10-25 wt.% of Fe2O3, 0.1-8.0 wt.5 of CaO, 0.1-8.0 wt.% of MgO, 0.5-7.0 wt.% of K2O, 0.1-5.0 wt.5 of Na2O, 0.1-5.0 wt.% of TiO2, and 0.01-2.0 wt.% of P2O5 or a natural mineral including the above ingredients, wherein a particle size of the natural mineral powder is 10-100,000 mesh. The anthracnose is controlled by treating the composition with a plant in need thereof.
    • 提供包含天然矿物作为有效成分的植物炭疽病控制组合物,以有效和环保的方法控制炭疽病,促进作物生长并恢复受污染的土壤。 用于控制炭疽病的组合物包括由40.0-60.0重量%的SiO 2,10.0-25.0重量%的Al 2 O 3,10-25重量%的Fe 2 O 3,0.1-8.0重量%的CaO,0.1-8.0重量% 的MgO,0.5-7.0重量%的K 2 O,0.1-5.0重量%的Na 2 O,0.1-5.0重量%的TiO 2和0.01-2.0重量%的P 2 O 5或包含上述成分的天然矿物, 其中所述天然矿物粉末的粒度为10-100,000目。 通过用需要的植物处理组合物来控制炭疽病。