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1. 发明专利

JP2003535566A Detection of the cells with the luciferase / luciferin reaction 审中-公开
公开(公告)号：JP2003535566A
公开(公告)日：2003-12-02
申请号：JP2000599895
申请日：2000-02-18
申请人：プロメガ・コーポレーション
发明人：ウッド，キース・ヴイ , シュルツ，ジョン・ダブリュー , プラット，タマラ・スー , ホール，メアリー・ピー
IPC分类号： G01N33/48 , C12Q1/04 , C12Q1/66 , C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6827 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107
摘要： (57)【要約】ＡＤＰ以外の高エネルギーリン酸供与体の存在下、触媒する内在性酵素によりＡＭＰおよび／またはＡＤＰをＡＴＰに変換し、次いで、そのＡＴＰをルシフェラーゼで検出する細胞の検出方法、およびその材料を開示する。

2. 发明专利

JP2008253251A Nucleic acid analog, diagnostic agent and method for using the same in analysis method 审中-公开
标题翻译：核酸类似物，诊断试剂及其在分析方法中的使用方法
公开(公告)号：JP2008253251A
公开(公告)日：2008-10-23
申请号：JP2008060342
申请日：2008-03-10
申请人： Rolf Henrik Berg , Buchardt Dorte , Michael Egholm , Peter Eigil Nielsen , エグホルム、ミカエル , ニールセン、ピーター・アイギル , ブカルト、ドルテ , ベルク、ロルフ・ヘンリク
发明人： BUCHARDT OLE , EGHOLM MICHAEL , NIELSEN PETER EIGIL , BERG ROLF HENRIK
IPC分类号： A61K31/70 , C12N15/09 , A61K38/00 , A61K48/00 , A61P31/12 , A61P43/00 , C07D519/00 , C07H21/00 , C07H21/04 , C07K1/22 , C07K5/06 , C07K5/078 , C07K5/08 , C07K5/10 , C07K7/06 , C07K7/08 , C07K9/00 , C07K14/00 , C07K16/00 , C08G69/00 , C12Q1/68 , G01N33/53
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： PROBLEM TO BE SOLVED: To provide a nucleic acid analog for performing capture, recognition, detection, identification or quantification about chemically or microbiologically individual units. SOLUTION: The nucleic acid analogues provided are: (a) a PNA (peptide nucleic acid) including a polyamide backbone bearing a plurality of ligands at respective spaced locations along the backbone, where each ligand being bound directly or indirectly to a nitrogen atom in the backbone and the ligand bearing nitrogen atoms mainly being separated from one another in the backbone by 4-8 intervening atoms; (b) a nucleic acid analog capable of hybridizing to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide and the nucleic acid; or (c) a nucleic acid analogue capable of hybridizing to double-stranded nucleic acid in which one strand has a sequence complementary to the analogue, so as to displace the other strand from one strand. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供用于进行关于化学或微生物学单个单元的捕获，识别，检测，鉴定或定量的核酸类似物。提供的核酸类似物是：（a）包含聚酰胺主链的PNA（肽核酸），其中所述聚酰胺主链在主链的相应间隔位置处具有多个配体，其中每个配体直接或间接地结合于氮骨架中的原子和带有氮原子的配体主要通过4-8个中间原子在骨架中彼此分离; （b）能够与互补序列的核酸杂交的核酸类似物，以形成与常规脱氧核糖核苷酸和核酸之间的杂交体相比对抗变性更稳定的杂交体; 或（c）能够与双链核酸杂交的核酸类似物，其中一条链具有与该类似物互补的序列，以便从一条链取代另一条链。版权所有（C）2009，JPO＆INPIT

3. 发明专利

JP2007306926A Nucleic acid analog, diagnostic agent and use of the same in analysis method 审中-公开
标题翻译：核酸类似物，诊断试剂及其在分析方法中的应用
公开(公告)号：JP2007306926A
公开(公告)日：2007-11-29
申请号：JP2007147536
申请日：2007-06-01
申请人： Rolf Henrik Berg , Buchardt Dorte , Michael Egholm , Peter Eigil Nielsen , エグホルム、ミカエル , ニールセン、ピーター・アイギル , ブカルト、ドルテ , ベルク、ロルフ・ヘンリク
发明人： BUCHARDT OLE , EGHOLM MICHAEL , NIELSEN PETER EIGIL , BERG ROLF HENRIK
IPC分类号： A61K31/70 , C12N15/09 , A61K38/00 , A61K48/00 , A61P31/12 , A61P43/00 , C07H21/00 , C07H21/04 , C07K1/22 , C07K5/06 , C07K5/078 , C07K5/08 , C07K5/10 , C07K7/06 , C07K7/08 , C07K9/00 , C07K14/00 , C07K16/00 , C08G69/00 , C12Q1/68 , G01N30/88 , G01N33/53 , G01N33/58
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： PROBLEM TO BE SOLVED: To obtain some nucleic acid analogs, e.g., for performing capture, recognition, detection, identification or quantification about one or more chemically or microbiologically individual units, in order to solve problems which can not be solved by conventional techniques in a field of diagnostic agents. SOLUTION: The nucleic acid homologs having a new structure and characteristics that have not been known such as a characteristic to form a hybrid with a conventionally known nucleic acid having a complementary sequence and enhancing stability in the Tm value of the hybrid higher than the hybrid formed by the conventionally known nucleic acid and/or a characteristic to enhance selectivity to a complementary sequence comparing with a sequence relating to a degree of mismatching of the known conventional nucleic acid, and applications of the nucleic acid homologs to diagnostic agents or analyses are provided. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：为了获得一些核酸类似物，例如用于对一个或多个化学或微生物学单个单元进行捕获，识别，检测，鉴定或定量，以解决常规的不能解决的问题技术在诊断剂领域。解决方案：具有新的结构和特征的核酸同系物，例如与常规已知的具有互补序列的核酸形成杂交体的特征，并提高混合物的Tm值的稳定性高于通过常规已知的核酸形成的杂合物和/或特征，以增强对互补序列的选择性与与已知的常规核酸的错配程度相关的序列以及核酸同系物与诊断试剂或分析的应用被提供。版权所有（C）2008，JPO＆INPIT

4. 发明专利

JPS59126246A Method for determining base arrangement of dna or dna partial, decomposition product 失效
标题翻译：用于确定DNA或DNA部分碱基配置的方法，分解产物
公开(公告)号：JPS59126246A
公开(公告)日：1984-07-20
申请号：JP132583
申请日：1983-01-08
申请人： Fuji Photo Film Co Ltd
发明人： SHIRAISHI HISASHI
IPC分类号： G03B42/02 , C12Q1/68 , G01N21/64 , G01N27/26 , G01N27/447 , G01N33/50 , G21H5/02
CPC分类号： C12Q1/6869 , C12Q2563/101 , C12Q2535/107
摘要： PURPOSE:To easily determine DNA constituting base arrangement, by transferring a separated and developed lines by superposing an accumulative fluorescent sheet to a gel body which is obtained by subjecting a substance wherein a radioactive label is applied to DNA or a DNA partial decomposition product to separation development while scanning the transferred sheet by an electromagnetic wave. CONSTITUTION:A developed line obtained by subjecting a substance wherein a radioactive label such as P is applied to DNA or a DNA partial decomposition product to separation development by using a crosslinked polyacrylamide type gel electropholetic medium is subjected to the absorption of radioactive energy and, thereafter, irradiated with an electromagnetic wave (exciting light) such as infrared rays. Subsequently, an accumulative fluorescent sheet capable of emitting exhaustive fluorescent is superposed to transfer the developed line to said sheet. The transferred sheet is scanned by irradiating the same with exciting light while the fluorescent thereof is detected and converted to an electric signal which is, in turn, subjected to A/D conversion and signal treatment so as to obtain a visible image which is then recorded by a recording apparatus through a storing means such as a magnetic tape. Further, image display is performed. By this method. The arrangement position of the specific split decomposition products of four constitutional bases such as DNA is easily determined.
摘要翻译：目的：为了容易地确定构成碱基排列的DNA，通过将累积的荧光片叠加在通过使放射性标记物的物质或DNA部分分解产物进行分离而获得的凝胶体上转印分离显影的线在通过电磁波扫描转印片材的同时进行显影。构成：通过使用交联的聚丙烯酰胺型凝胶电泳介质对其中对DNA或DNA部分分解产物施加放射性标记如32 P的物质进行分离显影获得的开发线经受吸收放射性能然后用诸如红外线的电磁波（激发光）照射。随后，将能够发出穷尽荧光的累积荧光片叠加以将显影线转移到所述片。通过在检测到荧光的同时照射转印的片材，并将其转换成电信号，该电信号又进行A / D转换和信号处理，以获得随后记录的可见图像通过记录装置通过诸如磁带的存储装置。此外，执行图像显示。通过这种方法。容易确定诸如DNA之类的四种宪法碱基的具体裂解分解产物的排列位置。

5. 发明专利

JP2011525111A Nucleic acid sequencing systems and methods according to phase synthesis 审中-公开
公开(公告)号：JP2011525111A
公开(公告)日：2011-09-15
申请号：JP2011514703
申请日：2009-06-10
申请人：ピーエルシーダイアグノスティクス, インコーポレイテッド
发明人：ルーヴェンデュワー， , ジェームズヘロン，
IPC分类号： C12M1/00 , C12Q1/68 , G01N21/64 , G01N33/53
CPC分类号： G02B27/56 , B01J2219/00529 , B01J2219/00596 , B01J2219/00659 , B01J2219/00704 , B01J2219/00711 , B01J2219/00722 , B01L3/502715 , B01L2300/0654 , B01L2300/0819 , B82Y30/00 , C12N15/1068 , C12Q1/6869 , C12Q1/6874 , G01N21/6452 , G01N21/648 , G01N2021/6441 , G02B6/24 , C12Q2523/319 , C12Q2535/107 , C12Q2565/607
摘要： A system and methods of sequencing a nucleic acid by detecting the identity of a fluorescent nucleotide analogue incorporated at the 3' end of a growing nucleic acid strand are provided. One method includes the steps of (a) immobilizing a plurality of complexes comprising a template nucleic acid, a primer configured to hybridize to the template and a polymerase, at a plurality of optical sensing sites of a substrate, wherein the substrate is part of a waveguide-based optical scanning system; (b) extending the primer by a single nucleotide with the polymerase and one or more fluorescent nucleotide analogues using a polymerase extension reaction, wherein each type of fluorescent nucleotide analogue comprises a unique fluorescent tag optionally configured to inhibit further primer extension and/or a blocking agent at the 3' end and wherein incorporation of the fluorescent nucleotide analogue reversibly terminates the polymerase extension reaction; (c) detecting the unique tag of the fluorescent nucleotide analogue by optically scanning the substrate using the optical scanning system to identify the fluorescent nucleotide analogue incorporated by the polymerase reaction; (d) recording the results of the optical scanning of the substrate; (e) reversing the termination of the polymerase extension reaction by providing a photo-cleaving pulse of light to one or more of the optical sensing sites of the substrate to cleave the fluorescent tag or the blocking agent; and (f) repeating steps (b) through (e).

6. 发明专利

JP2009519041A Probe and using methods for nucleic acid sequencing 审中-公开
公开(公告)号：JP2009519041A
公开(公告)日：2009-05-14
申请号：JP2008545768
申请日：2006-12-12
申请人：アメリカ合衆国
发明人：トーマスディー．シュナイダー , ダニエルニードル , イリヤジー．リャーホフ
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： G01N33/542 , C12Q1/6818 , C12Q1/6869 , Y10T436/143333 , C12Q2563/107 , C12Q2525/197 , C12Q2521/30 , C12Q2565/101 , C12Q2535/107
摘要：核酸分子の配列決定のためのナノプローブおよびナノプローブを使用するための方法を提供する。特定の例において、該プローブは、重合剤と、標的核酸分子内の相補的ヌクレオチドと特異的に結合することによって、リンカーから分離することなく、核酸分子の鋳型鎖に可逆的に結合することができる化学的部分を有する、１つまたは複数の分子リンカーとを含む。標的核酸分子内の相補的ヌクレオチドとリンカー上の化学的部分との可逆的な結合は、相補的ヌクレオチドとリンカー上の化学的部分との対合を示す特性シグナルの放出によって示される。かかる化学的部分の１つの例は、非加水分解性ヌクレオチド類似体である。特定の例において、重合剤および化学的部分は、化学的部分の特定の型を特徴とする供与体フルオロフォア、および受容体フルオロフォアなどのタグに関連する。

7. 发明专利

JPS59126252A Method for determining base arrangement of dna or dna partial decomposition product 失效
标题翻译：用于确定DNA或DNA部分分解产物的基因安排的方法
公开(公告)号：JPS59126252A
公开(公告)日：1984-07-20
申请号：JP133583
申请日：1983-01-08
申请人： Fuji Photo Film Co Ltd
发明人： SHIRAISHI HISASHI
IPC分类号： G01N27/26 , C12N15/09 , C12Q1/68 , G01N27/447 , G01N33/50 , G21H5/02
CPC分类号： C12Q1/6869 , C12Q2563/101 , C12Q2535/107
摘要： PURPOSE:To easily determine base arrangement, in performing the separative development of DNA split decomposition products having radioactive label applied thereto on a support medium for electrophoretic separation, by separating and developing a mixture comprising specific split decomposition products of four DNA constitutional bases on the same support medium as internal reference. CONSTITUTION:Respective specific split and decomposed products to which radioactive labels are applied of four bases, that is, guanine G, adenine A, thymine T and cytosine C are mixed to be used internal reference substances. One or more of specific split and decomposed products of bases to be measured to which radioactive labels are applied are developed on one support medium so as to simultaneously form separated and developed lines in parallel to the intenal reference substances. By this method, for example, the developed line 1 of G, the developed line 2 of G+A, the developed line 4 of T and C and the developed line 5 of C are obtained on one support medium along with the developed line 3 of the internal reference substances. These developed lines are transferred to an X-ray film to be made visible. By this method, the determination of base arrangement is performed easily and accurately.
摘要翻译：目的：为了容易地确定碱基排列，通过分离和显影含有四个DNA结构碱基的特异性裂解分解产物的混合物，在其上施加放射性标记的DNA裂解分解产物在电泳分离的支持介质上进行分离开发支持媒介作为内部参考。构成：将四种碱基（即鸟嘌呤G，腺嘌呤A，胸腺嘧啶T和胞嘧啶C）四种基因的放射性标记物分别分解和分解的产物混合使用内参照物质。在一个支撑介质上开发一种或多种待测量的待测量的碱的分解和分解的产物，以同时形成与内标参考物质平行的分离和显影的线。通过该方法，例如，在显影线3上，在一个支撑介质上获得G的开发线1，G + A的开发线2，T和C的显影线4和C的显影线5 的内部参考物质。将这些显影的线转移到X射线胶片以使其可见。通过这种方法，可以容易且准确地进行基本布置的确定。

8. 发明专利

JP2015521030A ナノ細孔の調製方法およびその使用审中-公开
标题翻译：制备和使用纳米孔
公开(公告)号：JP2015521030A
公开(公告)日：2015-07-27
申请号：JP2015505831
申请日：2013-04-08
申请人：ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨークThe Trustees Of Columbia University In The City Of New York , ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨークThe Trustees Of Columbia University In The City Of New York , ガバメント・オブ・ザ・ユナイテッド・ステイツ・オブ・アメリカ・アズ・リプリゼンテッド・バイ・ザ・セクレタリー・オブ・コマースGovernment Of The United States Of America, As Represented By The Secretary Of Commerce , ガバメント・オブ・ザ・ユナイテッド・ステイツ・オブ・アメリカ・アズ・リプリゼンテッド・バイ・ザ・セクレタリー・オブ・コマースGovernment Of The United States Of America, As Represented By The Secretary Of Commerce
发明人：ジュ、ジンイェ , クマー、シフ , タオ、チュアンジュアン , チェン、ミンチェン , ルッソ、ジェームズ・ジェイ． , カシアノビクズ、ジョン・ジェイ． , ロバートソン、ジョセフ・ダブリュ．エフ．
IPC分类号： C12M1/00 , C07H19/06 , C07H19/16 , C08J9/36 , C12N15/09 , C12Q1/68 , G01N27/00
CPC分类号： C12Q1/6883 , B82Y5/00 , C07H19/06 , C07H19/16 , C12Q1/42 , C12Q1/48 , C12Q1/6869 , C12Q2600/158 , G01N33/48721 , G01N2333/9125 , C12Q2565/631 , C12Q2521/525 , C12Q2521/543 , C12Q2535/107
摘要：本開示は、ヌクレオチド類似体および組み込まれたヌクレオチド類似体からのナノ細孔を通じたタグの移行を用いて核酸分子を配列決定するシステムおよび方法を提供する。態様では、本開示は、タグ分子および分子の組み込みから遊離したタグのナノ細孔を通じた移行の検出を用いて核酸分子を配列決定する組成物、方法、システムに関する。
摘要翻译：本公开提供了一种用于通过由核苷酸类似物和掺入的核苷酸类似物的纳米孔测序与过渡标签的核酸分子的系统和方法。在一些方面，本公开涉及组合物用于测序使用迁移的检测通过的标签从所述标签分子和分子，方法和系统的掺入释放的纳米孔的核酸分子。

9. 发明专利

JP2009069158A Nucleic acid analogue, diagnostic agent, and use of them in analyzing method 审中-公开
标题翻译：核酸类似物，诊断试剂及其在分析方法中的应用
公开(公告)号：JP2009069158A
公开(公告)日：2009-04-02
申请号：JP2008258890
申请日：2008-10-03
申请人： Rolf Henrik Berg , Buchardt Dorte , Michael Egholm , Peter Eigil Nielsen , エグホルム、ミカエル , ニールセン、ピーター・アイギル , ブカルト、ドルテ , ベルク、ロルフ・ヘンリク
发明人： BUCHARDT OLE , EGHOLM MICHAEL , NIELSEN PETER EIGIL , BERG ROLF HENRIK
IPC分类号： A61K31/70 , G01N33/531 , A61K38/00 , A61K48/00 , A61P31/12 , A61P43/00 , C07D239/47 , C07D239/54 , C07D473/18 , C07D473/34 , C07H21/00 , C07H21/04 , C07K1/22 , C07K5/06 , C07K5/078 , C07K5/08 , C07K5/10 , C07K7/06 , C07K7/08 , C07K9/00 , C07K14/00 , C07K16/00 , C08G69/00 , C12N15/09 , C12Q1/68 , G01N33/53 , G01N33/566
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： PROBLEM TO BE SOLVED: To provide some nucleic acid analogues in order to perform capture, recognition, detection, identification or quantification about one or more chemically or microbiologically individual units for example. SOLUTION: The nucleic acid analogue with a new structure having characteristics that have not been conventionally known such as a characteristic to form a hybrid with a conventionally known nucleic acid having a complementary arrangement and to make stability in the Tm value of the hybrid higher than the hybrid formed by the conventionally known nucleic acid having an equivalent arrangement and/or a characteristic to make selectivity to the complementary arrangement compared with an arrangement relating to a degree of mismatching higher than the known conventional nucleic acid having the equivalent arrangement, and the method of using the nucleic acid analogues for diagnostic agents or analysis are provided. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一些核酸类似物以便例如关于一个或多个化学或微生物学个体单元的捕获，识别，检测，鉴定或定量。解决方案：具有新的结构的核酸类似物具有以往未知的特征，例如与具有互补排列的常规已知核酸形成杂交体的特征，并使混合物的Tm值稳定高于由具有等同排列和/或特征的常规已知核酸形成的杂交，以使得与相对于具有等同排列的已知常规核酸的错配程度相关的排列相比，对互补排列具有选择性;以及提供了使用核酸类似物进行诊断或分析的方法。版权所有（C）2009，JPO＆INPIT

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