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    • 8. 发明专利
    • Cell mass formable of primitive organ-like form comprising somatic-derived plurality of cell species
    • 具有包含细胞种类的SOMATIC衍生多样性的初级有机体形式的细胞质量
    • JP2013078344A
    • 2013-05-02
    • JP2013019652
    • 2013-02-04
    • Shiseido Co Ltd株式会社 資生堂
    • FUJIWARA SHIGEYOSHIKISHIMOTO JIROSOMA TSUTOMU
    • C12N5/071C12N5/077C12N15/09C12Q1/02C12Q1/68
    • C12N5/0697C12N5/0625C12N5/0627C12N5/0629C12N5/0666C12N2501/40C12N2501/415
    • PROBLEM TO BE SOLVED: To provide a cell mass formable of a primitive organ-like form by combining a plurality of differentiated somatic cells and culturing the cells.SOLUTION: There is provided a method for producing a cell mass formable of a primitive organ-like form comprising a plurality of somatic-derived somatic cell species by the steps of: preparing a culture liquid including the plurality of kinds of somatic cells; mixing the culture liquid including the plurality of kinds of somatic cells; adding a Wnt signal activator to the mixed cell culture liquid; consigning the culture liquid containing the Wnt signal activator to a nonplanar contact culture over a predetermined period of time; exchanging the medium of culture product cultured by the nonplanar contact culture with a medium containing no Wnt signal activator; and further culturing for a predetermined period of time. In this case, at least one kind of the plurality of somatic cells holds the undifferentiated status.
    • 待解决的问题:通过组合多个分化的体细胞并培养细胞来提供可形成原始器官样形式的细胞团。 解决方案:提供了一种通过以下步骤制备包含多种体细胞衍生的体细胞种类的原始器官状形式的细胞块形成方法:制备包括多种体细胞的培养液 ; 混合包含多种体细胞的培养液; 向混合细胞培养液中加入Wnt信号激活剂; 将含有Wnt信号激活剂的培养液在预定时间段内进行非平面接触培养; 用不含Wnt信号激活剂的培养基更换由非平面接触培养物培养的培养产物的培养基; 并进一步培养预定的时间。 在这种情况下,多个体细胞中的至少一种保持未分化状态。 版权所有(C)2013,JPO&INPIT